##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546666_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1852516
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23986405515526	31.0	31.0	33.0	30.0	34.0
2	31.618457276482363	31.0	31.0	34.0	30.0	34.0
3	31.590058061576798	31.0	31.0	34.0	30.0	34.0
4	35.447716510950514	37.0	35.0	37.0	33.0	37.0
5	35.371660487682696	37.0	35.0	37.0	33.0	37.0
6	35.459509661455016	37.0	35.0	37.0	33.0	37.0
7	35.93002111722652	37.0	35.0	37.0	35.0	37.0
8	35.92830885131357	37.0	35.0	37.0	35.0	37.0
9	37.631946498707705	39.0	37.0	39.0	35.0	39.0
10	37.0278680454042	39.0	37.0	39.0	33.0	39.0
11	36.7520150973055	39.0	35.0	39.0	32.0	39.0
12	36.18877461787105	38.0	35.0	39.0	32.0	39.0
13	35.9619587631092	38.0	35.0	39.0	31.0	39.0
14	36.98717473965137	39.0	35.0	41.0	31.0	41.0
15	37.236646808988425	39.0	35.0	41.0	32.0	41.0
16	37.33677927747993	39.0	35.0	41.0	32.0	41.0
17	37.2745574127295	39.0	35.0	41.0	32.0	41.0
18	37.10968218358168	39.0	35.0	40.0	32.0	41.0
19	36.978239864055155	38.0	35.0	40.0	32.0	41.0
20	36.74887018519678	38.0	35.0	40.0	32.0	41.0
21	36.624186241846225	38.0	35.0	40.0	31.0	41.0
22	36.51846137901103	38.0	35.0	40.0	31.0	41.0
23	36.46164621520138	38.0	35.0	40.0	31.0	41.0
24	36.324982888137	38.0	35.0	40.0	31.0	41.0
25	36.22688656940075	37.0	35.0	40.0	31.0	41.0
26	36.07689704164498	37.0	35.0	40.0	31.0	41.0
27	35.96151558205165	37.0	35.0	40.0	30.0	41.0
28	35.88021695898983	37.0	35.0	40.0	30.0	41.0
29	35.83961002226162	37.0	35.0	40.0	30.0	41.0
30	35.75948385870891	36.0	35.0	40.0	30.0	41.0
31	35.602136769668924	36.0	35.0	40.0	30.0	41.0
32	35.28004400501804	36.0	34.0	40.0	29.0	41.0
33	35.01648352834739	36.0	34.0	40.0	27.0	41.0
34	34.75668820134347	36.0	34.0	40.0	25.0	41.0
35	34.56365235172058	36.0	34.0	40.0	24.0	41.0
36	34.39349241787925	36.0	34.0	40.0	23.0	41.0
37	34.33051860280829	36.0	33.0	40.0	23.0	41.0
38	34.267709968496895	36.0	33.0	40.0	23.0	41.0
39	34.21024703700265	36.0	33.0	40.0	23.0	41.0
40	34.08447484394197	36.0	33.0	40.0	23.0	41.0
41	34.04961738522096	36.0	33.0	40.0	22.0	41.0
42	33.96269074059279	36.0	33.0	40.0	22.0	41.0
43	33.82433134180757	35.0	33.0	40.0	21.0	41.0
44	33.674014691371084	35.0	33.0	40.0	21.0	41.0
45	33.5695308434583	35.0	33.0	40.0	20.0	41.0
46	33.54302203057895	35.0	33.0	40.0	20.0	41.0
47	33.50022779830242	35.0	33.0	40.0	20.0	41.0
48	33.4010934318516	35.0	33.0	40.0	20.0	41.0
49	33.35757801821955	35.0	33.0	40.0	20.0	41.0
50	33.23815772711275	35.0	33.0	39.0	20.0	41.0
51	32.907427520194155	35.0	32.0	39.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	7.0
14	16.0
15	46.0
16	134.0
17	388.0
18	930.0
19	1874.0
20	3403.0
21	5332.0
22	8075.0
23	12492.0
24	18780.0
25	28953.0
26	38889.0
27	43123.0
28	42746.0
29	44784.0
30	50467.0
31	59956.0
32	72801.0
33	93651.0
34	152198.0
35	257783.0
36	160522.0
37	170994.0
38	215745.0
39	368149.0
40	277.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.015179356075738	23.463495052134505	28.03673490539353	18.48459068639623
2	34.188206741534216	25.273735827382865	27.71781728200998	12.820240149072937
3	25.562424292151864	24.63249980027163	36.75406852086568	13.05100738671083
4	23.278071552418442	27.149131235573677	35.50214950910006	14.070647702907829
5	20.575206907794588	31.137976676044904	34.491469979206656	13.795346436953851
6	18.754170004469596	39.1177728019623	31.389526460230304	10.738530733337797
7	82.02428481049556	3.7830712393307264	12.221109021460544	1.9715349287131663
8	82.31702182329329	3.008395069192385	11.596445050946928	3.078138056567393
9	76.52678843259653	5.220683654014325	14.801437612414684	3.4510903009744585
10	40.52305081305641	27.463568465805423	20.07728948090057	11.936091240237602
11	34.37184888011763	22.185719313625363	29.370380606699214	14.072051199557793
12	31.999615657840476	21.502594309576814	30.77053045695692	15.7272595756258
13	19.98298530215124	34.77098173511052	31.8091179779284	13.436914984809848
14	14.782544388280586	38.35934480457929	32.10601150003563	14.7520993071045
15	13.134137572900856	26.386384786960004	46.84585720177316	13.633620438365984
16	14.952205541004773	23.118882643928583	44.93963884792358	16.989272967143062
17	15.080679465116631	24.21037119247553	32.404956286477415	28.30399305593042
18	17.88583742326652	26.256615327478954	37.276277235932106	18.58127001332242
19	23.21275497755485	29.653617026789515	29.578206072174275	17.55542192348136
20	25.220241012763182	27.24224784023458	28.427608722407793	19.109902424594445
21	18.95999818625048	32.189087705585266	30.91924712121245	17.9316669869518
22	18.93149640812819	27.801757177805754	29.243040276035405	24.023706138030658
23	15.833061630776738	33.269834106695974	28.190363807923923	22.70674045460336
24	16.480613392812803	28.931679942305493	38.29435211355799	16.293354551323713
25	16.066365958512637	29.81723234779079	35.19424393635467	18.922157757341907
26	15.347343828609308	35.531407016187714	30.28384100326259	18.837408151940387
27	16.062641294326202	34.56482966948734	30.55055934739565	18.821969688790812
28	14.659792412049343	31.40064647214923	37.01279773022203	16.926763385579395
29	14.450995295047383	28.53362670012027	36.436608374772476	20.578769630059877
30	17.29572106259811	31.704449516225502	32.73499392177989	18.264835499396494
31	23.88233084086723	30.21717491238942	27.842836445137316	18.05765780160603
32	23.65798729943493	29.662307909891194	28.719104180476712	17.960600610197158
33	22.109120784921696	30.375554111273534	28.15700377216715	19.358321331637622
34	16.3372408119552	30.234232794750493	30.9043484644667	22.524177928827605
35	16.741393866503717	29.799796600947037	32.71734225237461	20.74146728017464
36	22.30841730921622	29.839526352269026	28.78139783947885	19.070658499035904
37	16.889354801793885	33.521383890881374	31.48836501277182	18.10089629455292
38	16.43424402272369	35.57383579952886	28.296975572680616	19.694944605066837
39	16.64358094612948	34.72655566807521	28.02345566785928	20.60640771793604
40	20.034968658840192	30.478387231203403	28.60509706798754	20.88154704196887
41	15.605803134763748	29.464306920965868	28.873488811972475	26.056401132297914
42	19.527928503721427	30.787696300598753	26.05926210623822	23.6251130894416
43	19.525013549140734	30.018094310656423	29.060801634101946	21.396090506100894
44	18.072016651947944	33.37358489751235	28.646230315959485	19.908168134580215
45	16.180696954844116	36.698846325753735	26.45197126502551	20.668485454376643
46	20.258124626184067	34.16758613690786	26.96019899423271	18.614090242675367
47	18.995247544420668	31.93068238007121	28.02259197761315	21.05147809789497
48	19.4857696235822	29.5000421048995	30.18143972845579	20.832748543062515
49	18.642268136955362	28.220916850380778	31.110284607528353	22.026530405135503
50	17.066843147373625	33.539575366690485	29.753427230857927	19.64015425507796
51	16.376646679434888	34.54545061958979	27.392314020499686	21.685588680475636
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3443.0
1	3347.5
2	3252.0
3	28161.0
4	53070.0
5	33379.5
6	13689.0
7	14807.0
8	15925.0
9	19381.5
10	22838.0
11	25876.5
12	28915.0
13	30684.0
14	32453.0
15	31761.5
16	31070.0
17	28970.0
18	26870.0
19	24323.5
20	21777.0
21	21174.0
22	20571.0
23	18952.5
24	17334.0
25	17548.5
26	19386.5
27	21010.0
28	21929.5
29	22849.0
30	26504.5
31	30160.0
32	32288.5
33	34417.0
34	38078.5
35	41740.0
36	47540.5
37	53341.0
38	59727.5
39	66114.0
40	86375.0
41	106636.0
42	126826.0
43	147016.0
44	155947.0
45	164878.0
46	175232.5
47	185587.0
48	183421.5
49	181256.0
50	161571.0
51	141886.0
52	121362.0
53	100838.0
54	84839.0
55	68840.0
56	60726.0
57	52612.0
58	44950.5
59	37289.0
60	32819.0
61	28349.0
62	24131.5
63	19914.0
64	17533.5
65	15153.0
66	11964.5
67	8776.0
68	7228.5
69	5681.0
70	4554.5
71	3428.0
72	2805.5
73	2183.0
74	1782.5
75	1073.5
76	765.0
77	692.0
78	619.0
79	488.0
80	357.0
81	278.5
82	200.0
83	198.5
84	197.0
85	117.5
86	38.0
87	27.0
88	16.0
89	12.5
90	9.0
91	6.0
92	3.0
93	4.5
94	6.0
95	3.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1852516.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.400023532145042
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.71885554958808	21.78817302967538
2	8.172147729945282	4.641783756772234
3	3.4776572816061826	2.9629664590305342
4	2.192171688194251	2.490309101248754
5	1.5154986669922725	2.152009890275749
6	1.0826564174862379	1.84484806403022
7	0.7693808156190025	1.5295303288132434
8	0.5975332115557941	1.3575965815538205
9	0.4699604380568096	1.2012198749991525
>10	3.793417740919443	22.818364730697656
>50	0.7871046636755598	15.921276880032655
>100	0.4180266935301053	17.046587915060048
>500	0.003276370625101658	0.63295215823007
>1k	0.0021200045221246027	1.2562162732219246
>5k	0.0	0.0
>10k+	1.927276838295093E-4	2.356164956358511
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43047	2.323704626572726	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4773	0.25764959654869374	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2972	0.16043046321867124	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTT	2477	0.13371004622902044	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTC	2233	0.12053876997553598	No Hit
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	2036	0.10990458382005878	TruSeq Adapter, Index 23 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT	1976	0.1066657453970708	TruSeq Adapter, Index 22 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0796128076626598E-4	0.0	0.0	0.11740789283331426	0.0
2	1.0796128076626598E-4	0.0	0.0	0.3852598304144202	0.0
3	1.0796128076626598E-4	0.0	0.0	0.6228826093809716	0.0
4	1.0796128076626598E-4	0.0	0.0	0.9656056951734829	0.0
5	1.0796128076626598E-4	0.0	0.0	1.6585551757717611	0.0
6	1.0796128076626598E-4	0.0	0.0	2.945129758663353	0.0
7	1.0796128076626598E-4	0.0	0.0	3.67975229363741	0.0
8	1.0796128076626598E-4	0.0	0.0	5.287241783606727	0.0
9	1.0796128076626598E-4	0.0	0.0	6.104184795165062	0.0
10	1.0796128076626598E-4	0.0	0.0	7.1795871128778375	0.0
11	1.0796128076626598E-4	0.0	0.0	7.92430402760354	0.0
12	1.0796128076626598E-4	0.0	0.0	8.570992099393473	0.0
13	1.0796128076626598E-4	0.0	0.0	8.931798699714335	0.0
14	1.0796128076626598E-4	0.0	0.0	9.092607027415688	0.0
15	1.0796128076626598E-4	0.0	0.0	9.253091471274741	0.0
16	1.0796128076626598E-4	0.0	0.0	9.54545061958979	0.0
17	1.0796128076626598E-4	0.0	0.0	9.858700275733112	0.0
18	1.0796128076626598E-4	0.0	0.0	10.368439462871036	0.0
19	1.0796128076626598E-4	0.0	0.0	10.558667239581197	0.0
20	1.0796128076626598E-4	0.0018353417730265218	0.0	10.76616882121396	0.0
21	1.0796128076626598E-4	0.0018353417730265218	0.0	11.004547329145876	0.0
22	1.0796128076626598E-4	0.0018353417730265218	0.0	11.261117312886906	0.0
23	1.0796128076626598E-4	0.0018353417730265218	0.0	11.548510242286707	0.0
24	1.0796128076626598E-4	0.0018353417730265218	0.0	11.777981944555405	0.0
25	1.0796128076626598E-4	0.0018353417730265218	0.0	11.964269134517597	0.0
26	1.0796128076626598E-4	0.0018353417730265218	0.0	12.150286421277873	0.0
27	1.0796128076626598E-4	0.0018353417730265218	0.0	12.351472267985809	0.0
28	1.0796128076626598E-4	0.0018353417730265218	0.0	12.547260050655433	0.0
29	1.0796128076626598E-4	0.0018353417730265218	0.0	12.754977554849729	0.0
30	1.0796128076626598E-4	0.0018353417730265218	0.0	13.077781784340864	0.0
31	1.6194192114939897E-4	0.0018353417730265218	0.0	13.306659699565348	0.0
32	1.6194192114939897E-4	0.0018353417730265218	0.0	13.505038552973362	0.0
33	1.6194192114939897E-4	0.0018353417730265218	0.0	13.708167702735091	0.0
34	1.6194192114939897E-4	0.0018353417730265218	0.0	13.9212832709677	0.0
35	1.6194192114939897E-4	0.0018353417730265218	0.0	14.163548385007202	0.0
36	1.6194192114939897E-4	0.0018353417730265218	0.0	14.37185967624571	0.0
37	1.6194192114939897E-4	0.0018353417730265218	0.0	14.58583893472445	0.0
38	1.6194192114939897E-4	0.0018353417730265218	0.0	14.806835676452996	0.0
39	1.6194192114939897E-4	0.0018353417730265218	0.0	15.054606815811578	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTCC	20	7.034407E-4	45.0	39
TACCCGC	20	7.034407E-4	45.0	25
TACGCCC	20	7.034407E-4	45.0	37
TCGTCGT	25	3.891733E-5	45.0	18
TCGTAAT	20	7.034407E-4	45.0	40
TCGCGTG	20	7.034407E-4	45.0	1
ATCGACG	25	3.891733E-5	45.0	1
CGCTTAA	20	7.034407E-4	45.0	20
CGTTTTT	15920	0.0	43.65735	1
ATTCGGC	235	0.0	41.17021	17
ACTACGG	110	0.0	40.90909	2
AACGCCG	210	0.0	40.714287	13
CGTAAGG	305	0.0	40.573772	2
GCGAGAC	145	0.0	40.34483	21
CGACGGT	95	0.0	40.263157	28
AAGGGCG	695	0.0	40.143887	5
AGTAACG	45	1.929402E-8	40.0	1
GTTGATC	1000	0.0	39.824997	16
AACACGT	130	0.0	39.807693	41
ACGTTAG	85	0.0	39.705883	1
>>END_MODULE