FastQCFastQC Report
Thu 26 May 2016
SRR1546665_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546665_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1800814
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174970.9716161691324036No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAG253.8916893E-545.01
CGTTTTT104850.040.793991
AGGGCGA19050.039.094496
GTACGAG1850.038.918921
TAGACGG1450.038.7931022
GTTGATC13300.037.89473316
ATAGGGC7250.037.241384
GGGCGAT39750.036.9056637
CATATGC13450.036.80297533
GCCAGTA13850.036.714825
GTCTCAA13450.036.6356942
CGTTCGG5000.036.4545
TTGATCC13850.036.38989317
GTAGCAT13850.036.06498329
TACGGGA4500.036.04
TAGCATA13750.036.030
CAGTAGC14100.035.90425527
ATATGCT13750.035.83636534
GGCGATT10200.035.7352948
TTACGGG5000.035.5500033
Produced by FastQC (version 0.11.5)