##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546665_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1800814
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.404116693895094	31.0	31.0	33.0	30.0	34.0
2	31.81505807929081	31.0	31.0	34.0	30.0	34.0
3	31.88017307728616	31.0	31.0	34.0	30.0	34.0
4	35.69970468910171	37.0	35.0	37.0	33.0	37.0
5	35.6382596981143	37.0	35.0	37.0	33.0	37.0
6	35.7006881332553	37.0	35.0	37.0	33.0	37.0
7	35.997541667268244	37.0	35.0	37.0	35.0	37.0
8	35.96370807867997	37.0	35.0	37.0	35.0	37.0
9	37.69094254042894	39.0	37.0	39.0	35.0	39.0
10	37.21597455372959	39.0	37.0	39.0	34.0	39.0
11	36.988994976716086	39.0	37.0	39.0	33.0	39.0
12	36.592036156982346	39.0	35.0	39.0	32.0	39.0
13	36.48859293630547	39.0	35.0	39.0	32.0	39.0
14	37.55566760365035	40.0	36.0	41.0	32.0	41.0
15	37.6877450974948	40.0	36.0	41.0	33.0	41.0
16	37.71570023333892	40.0	36.0	41.0	33.0	41.0
17	37.62710474263305	40.0	36.0	41.0	33.0	41.0
18	37.476210758023875	39.0	36.0	41.0	32.0	41.0
19	37.374282407844454	39.0	36.0	41.0	32.0	41.0
20	37.20084695032357	39.0	35.0	41.0	32.0	41.0
21	37.123829557078075	39.0	35.0	41.0	32.0	41.0
22	37.01868877074479	39.0	35.0	41.0	32.0	41.0
23	36.927967574663455	38.0	35.0	41.0	32.0	41.0
24	36.78477677317036	38.0	35.0	41.0	31.0	41.0
25	36.661365360331494	38.0	35.0	41.0	31.0	41.0
26	36.51491381119871	38.0	35.0	40.0	31.0	41.0
27	36.409390420110014	38.0	35.0	40.0	31.0	41.0
28	36.26665496825324	38.0	35.0	40.0	30.0	41.0
29	36.282990914108844	38.0	35.0	40.0	30.0	41.0
30	36.230460225209264	38.0	35.0	40.0	30.0	41.0
31	36.128500222677076	38.0	35.0	40.0	30.0	41.0
32	35.99507556027441	38.0	35.0	40.0	30.0	41.0
33	35.78520380228053	38.0	35.0	40.0	29.0	41.0
34	35.53345820279051	38.0	35.0	40.0	27.0	41.0
35	35.33608801353166	38.0	34.0	40.0	26.0	41.0
36	35.20260171233675	38.0	34.0	40.0	26.0	41.0
37	35.14828794089784	38.0	34.0	40.0	26.0	41.0
38	35.056785431477095	38.0	34.0	40.0	25.0	41.0
39	34.934675652232826	38.0	34.0	40.0	25.0	41.0
40	34.78078302367707	37.0	34.0	40.0	24.0	41.0
41	34.69900278429643	37.0	34.0	40.0	24.0	41.0
42	34.6008238496591	37.0	34.0	40.0	23.0	41.0
43	34.48418715092175	37.0	34.0	40.0	23.0	41.0
44	34.31472933906556	36.0	33.0	40.0	23.0	41.0
45	34.18961702874367	36.0	33.0	40.0	23.0	41.0
46	34.14468901285752	36.0	33.0	40.0	23.0	41.0
47	34.094427297877516	36.0	33.0	40.0	23.0	41.0
48	33.971765546025296	36.0	33.0	40.0	23.0	41.0
49	33.87582726478137	35.0	33.0	40.0	23.0	41.0
50	33.775904118915115	35.0	33.0	40.0	23.0	41.0
51	33.419132681109765	35.0	33.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	9.0
12	6.0
13	15.0
14	27.0
15	55.0
16	149.0
17	358.0
18	770.0
19	1552.0
20	2575.0
21	4268.0
22	6759.0
23	9907.0
24	14623.0
25	22145.0
26	30570.0
27	35743.0
28	37526.0
29	39976.0
30	44639.0
31	53225.0
32	64579.0
33	84144.0
34	136258.0
35	188501.0
36	152385.0
37	191637.0
38	266499.0
39	411686.0
40	223.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.60802392695747	22.685685473347053	26.919104360583603	16.78718623911187
2	34.98012565428745	24.64291148336252	27.277886555746456	13.09907630660357
3	29.02942780320455	24.65285143274097	32.33410002365597	13.983620740398509
4	25.45093496607645	27.308483830090168	31.75391795043797	15.486663253395408
5	22.47616911019128	31.83388178901319	30.890697206929758	14.799251893865776
6	21.582128970565535	39.199328747999516	28.460740531781738	10.757801749653211
7	84.30004431329388	4.252521359785074	9.420184427708802	2.027249899212245
8	84.23351884203477	3.6894981935946745	9.620205085033769	2.456777879336789
9	78.14282874300179	5.73790519176328	12.161889012413276	3.9573770528216685
10	43.240168057334074	24.14119392674646	18.645512529333956	13.973125486585511
11	34.01228555530999	24.67650740165281	24.960101376377573	16.35110566665963
12	29.652923622317463	21.41492680532248	30.262037056575526	18.670112515784528
13	24.196391187540744	25.286120609901968	31.41290549718072	19.10458270537657
14	21.416092944635036	27.64694188294849	32.20471409040579	18.73225108201069
15	20.232905785939025	22.417528961902782	38.15113609734265	19.198429154815543
16	23.64652873644918	21.041206920870227	35.316029306746834	19.996235035933747
17	23.811842866614764	20.99728234009731	30.774360927891497	24.41651386539643
18	25.573546185225126	21.545256756111403	32.33237857990886	20.548818478754608
19	28.456187035418427	22.92302258867379	28.20124676951645	20.419543606391333
20	28.638715603055065	23.508146871359283	27.711079545139032	20.14205798044662
21	26.66577447754182	24.30784078755496	29.205625900287313	19.8207588346159
22	25.661672998988234	22.110612200926912	29.241387505872346	22.986327294212504
23	24.21305032057725	24.48531608483719	28.036321352455058	23.265312242130502
24	23.907521820687755	23.253706379448406	31.931004534616015	20.907767265247827
25	23.78568802774745	23.53552337998261	30.125931939667282	22.552856652602657
26	21.812191597799664	25.881351433296274	29.237722496604313	23.06873447229975
27	22.182857307861887	24.977537935622447	30.114437137872095	22.725167618643567
28	21.90253962930097	25.075771290094366	32.03934442979674	20.982344650807914
29	23.189901899918592	23.66196619972968	31.624920730291965	21.52321117005976
30	23.844272645592493	24.187117603483756	31.084165271927027	20.88444447899672
31	27.189815272426802	23.979045031857815	27.83119189433223	20.999947801383154
32	27.713522884651056	23.75842258001104	27.835134555817536	20.692919979520372
33	27.41132621136886	23.9030238547679	27.090526839529232	21.595123094334006
34	24.561004079266375	25.277235738949166	28.766602214331964	21.395157967452498
35	24.498032556388388	25.17983534112907	29.317797396066446	21.0043347064161
36	27.193147099034103	25.64701296191611	26.854244802628145	20.30559513642164
37	24.721598121738282	26.951367548230966	27.854348089252973	20.472686240777783
38	25.445437452174406	26.87634591912324	26.16305737294357	21.515159255758785
39	24.19300383048999	26.43276873680458	26.478803474428787	22.895423958276645
40	25.17311615747101	24.697220257061527	27.42148828252113	22.708175302946334
41	22.632987082508244	24.28546201884259	28.138275246638468	24.9432756520107
42	23.96027574197002	25.00058306965628	27.191092472626266	23.84804871574743
43	23.899914149934418	24.094992597791887	28.474512081758586	23.53058117051511
44	23.38020472963893	25.31971652819225	28.351123436401537	22.948955305767278
45	22.192408544136153	26.98229800523541	27.562813261114137	23.2624801895143
46	23.932066276694872	26.48246848369682	27.271111841644945	22.314353397963366
47	23.21666757366391	26.47574930003876	28.259387143813854	22.048195982483477
48	23.753480370543542	25.20443532757964	28.889435555254455	22.15264874662236
49	23.814952571448245	23.958332176449098	29.055582642071865	23.1711326100308
50	22.94351332230869	26.075263741841187	28.240951036586786	22.740271899263334
51	22.993268599644384	27.127898827974462	26.91344025535119	22.965392317029966
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1779.0
1	2276.0
2	2773.0
3	16144.0
4	29515.0
5	20544.0
6	11573.0
7	11621.0
8	11669.0
9	12177.0
10	12685.0
11	12701.5
12	12718.0
13	12495.0
14	12272.0
15	11932.5
16	11593.0
17	10976.5
18	10360.0
19	10016.5
20	9673.0
21	9910.0
22	10147.0
23	10122.5
24	10098.0
25	10779.5
26	13305.5
27	15150.0
28	16814.5
29	18479.0
30	20429.0
31	22379.0
32	26493.0
33	30607.0
34	34929.0
35	39251.0
36	41570.5
37	43890.0
38	49598.5
39	55307.0
40	65444.0
41	75581.0
42	85408.5
43	95236.0
44	100301.5
45	105367.0
46	107509.0
47	109651.0
48	112426.5
49	115202.0
50	121111.5
51	127021.0
52	123703.0
53	120385.0
54	113237.0
55	106089.0
56	101194.5
57	96300.0
58	93574.0
59	90848.0
60	87383.5
61	83919.0
62	79224.0
63	74529.0
64	68870.0
65	63211.0
66	55681.0
67	48151.0
68	41494.0
69	34837.0
70	30554.0
71	26271.0
72	22027.5
73	17784.0
74	15171.5
75	9808.0
76	7057.0
77	5336.0
78	3615.0
79	2739.5
80	1864.0
81	1484.5
82	1105.0
83	785.5
84	466.0
85	352.0
86	238.0
87	162.0
88	86.0
89	64.5
90	43.0
91	27.5
92	12.0
93	8.5
94	5.0
95	4.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1800814.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.712596953831568
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.06081091309508	19.55720974990007
2	6.243629105341535	3.2107983742971773
3	2.6934850896906317	2.0776948953711023
4	1.627777693961933	1.674175671011223
5	1.2022878403944792	1.5456971331277911
6	0.9386611564378857	1.4481249595022878
7	0.7888367464223871	1.4198128926191471
8	0.675077966896791	1.3886406140183387
9	0.6136939535679189	1.420169875298579
>10	8.250142501601749	49.208691703582225
>50	0.8576899741108476	13.89867593249312
>100	0.04638274495720462	1.874205622331341
>500	8.710362980654719E-4	0.14860147592872985
>1k	4.3551814903273594E-4	0.14781759474447884
>5k	0.0	0.0
>10k+	2.1775907451636797E-4	0.9796835057742977
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17497	0.9716161691324036	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.553044345501534E-5	0.0	0.0	0.02493316911130189	0.0
2	5.553044345501534E-5	0.0	0.0	0.08568347425108867	0.0
3	5.553044345501534E-5	0.0	0.0	0.15032091043272652	0.0
4	5.553044345501534E-5	0.0	0.0	0.23250596674614923	0.0
5	5.553044345501534E-5	0.0	0.0	0.38388195560452104	0.0
6	5.553044345501534E-5	0.0	0.0	0.7566022920745841	0.0
7	5.553044345501534E-5	0.0	0.0	0.9629534199534211	0.0
8	5.553044345501534E-5	0.0	0.0	1.4607283150841786	0.0
9	5.553044345501534E-5	0.0	0.0	1.8195105102470328	0.0
10	5.553044345501534E-5	0.0	0.0	2.3186736664641656	0.0
11	5.553044345501534E-5	0.0	0.0	2.6520229185246227	0.0
12	5.553044345501534E-5	0.0	0.0	2.934950527927926	0.0
13	5.553044345501534E-5	0.0	0.0	3.1064285373170133	0.0
14	5.553044345501534E-5	0.0	0.0	3.2018853696161846	0.0
15	5.553044345501534E-5	0.0	0.0	3.2786284424710157	0.0
16	5.553044345501534E-5	0.0	0.0	3.388301068294671	0.0
17	5.553044345501534E-5	0.0	0.0	3.499806198752342	0.0
18	5.553044345501534E-5	0.0	0.0	3.6705623123765143	0.0
19	5.553044345501534E-5	0.0	0.0	3.7587446565830787	0.0
20	5.553044345501534E-5	0.0	0.0	3.85442361065607	0.0
21	5.553044345501534E-5	0.0	0.0	3.951379764928527	0.0
22	5.553044345501534E-5	0.0	0.0	4.059275416561621	0.0
23	5.553044345501534E-5	0.0	0.0	4.181664513936475	0.0
24	5.553044345501534E-5	0.0	0.0	4.2824522688073285	0.0
25	5.553044345501534E-5	0.0	0.0	4.372966891639003	0.0
26	5.553044345501534E-5	0.0	0.0	4.464425532009414	0.0
27	5.553044345501534E-5	0.0	0.0	4.5733762620681535	0.0
28	5.553044345501534E-5	0.0	0.0	4.678384330641587	0.0
29	5.553044345501534E-5	0.0	0.0	4.7803382248249955	0.0
30	5.553044345501534E-5	0.0	0.0	4.931991865900643	0.0
31	5.553044345501534E-5	0.0	0.0	5.041386839507023	0.0
32	5.553044345501534E-5	0.0	0.0	5.168218372358278	0.0
33	5.553044345501534E-5	0.0	0.0	5.282444494545245	0.0
34	5.553044345501534E-5	0.0	0.0	5.4026679046253525	0.0
35	5.553044345501534E-5	0.0	0.0	5.5343305860571945	0.0
36	5.553044345501534E-5	0.0	0.0	5.654776117911123	0.0
37	1.1106088691003068E-4	0.0	0.0	5.786549860229874	0.0
38	1.1106088691003068E-4	0.0	0.0	5.939147518844256	0.0
39	1.1106088691003068E-4	0.0	0.0	6.171653485590405	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTAG	25	3.8916893E-5	45.0	1
CGTTTTT	10485	0.0	40.79399	1
AGGGCGA	1905	0.0	39.09449	6
GTACGAG	185	0.0	38.91892	1
TAGACGG	145	0.0	38.793102	2
GTTGATC	1330	0.0	37.894733	16
ATAGGGC	725	0.0	37.24138	4
GGGCGAT	3975	0.0	36.905663	7
CATATGC	1345	0.0	36.802975	33
GCCAGTA	1385	0.0	36.7148	25
GTCTCAA	1345	0.0	36.63569	42
CGTTCGG	500	0.0	36.45	45
TTGATCC	1385	0.0	36.389893	17
GTAGCAT	1385	0.0	36.064983	29
TACGGGA	450	0.0	36.0	4
TAGCATA	1375	0.0	36.0	30
CAGTAGC	1410	0.0	35.904255	27
ATATGCT	1375	0.0	35.836365	34
GGCGATT	1020	0.0	35.735294	8
TTACGGG	500	0.0	35.550003	3
>>END_MODULE