FastQCFastQC Report
Thu 26 May 2016
SRR1546664_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546664_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2405230
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111000.46149432694586384No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC107340.44627748697629754No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAA207.034825E-445.00000423
GTAATCG207.034825E-445.0000049
TACGCTA302.1664946E-645.00000410
CGTAGTC406.8212103E-945.00000411
CTACCGT207.034825E-445.00000429
CACGTAG302.1664946E-645.0000041
TAGTCGA406.8212103E-945.00000416
ACGACCG207.034825E-445.00000438
CGTGCAA253.892082E-545.025
CAAATCG253.892082E-545.019
GAACGTA253.892082E-545.036
CAATCGA351.2126657E-745.018
TTCGATC351.2126657E-745.015
TTTAGCG2300.043.0434761
CCAACGG800.042.1875042
CGTAAGG3850.042.0779232
TAGTCCG750.042.01
GTTCCGA1400.041.7857139
TCTAGCG650.041.538461
CGTTTTT77250.041.2135931
Produced by FastQC (version 0.11.5)