##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546664_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2405230
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.365723860088224	31.0	31.0	33.0	30.0	34.0
2	31.777767198978893	31.0	31.0	34.0	30.0	34.0
3	31.88022891781659	31.0	31.0	34.0	30.0	34.0
4	35.62572186443708	37.0	35.0	37.0	33.0	37.0
5	35.530268622959134	37.0	35.0	37.0	33.0	37.0
6	35.58724986799599	37.0	35.0	37.0	33.0	37.0
7	35.96112721028758	37.0	35.0	37.0	35.0	37.0
8	35.9466932476312	37.0	35.0	37.0	35.0	37.0
9	37.670942903589264	39.0	37.0	39.0	35.0	39.0
10	37.151294886559704	39.0	37.0	39.0	33.0	39.0
11	36.84369644483064	39.0	37.0	39.0	32.0	39.0
12	36.03654744036953	37.0	35.0	39.0	32.0	39.0
13	35.734574656062	37.0	35.0	39.0	31.0	39.0
14	36.71970622352124	38.0	35.0	41.0	31.0	41.0
15	37.01020359799271	38.0	35.0	41.0	32.0	41.0
16	37.13997621849054	38.0	35.0	41.0	32.0	41.0
17	37.083016593007734	38.0	35.0	41.0	32.0	41.0
18	37.01370471846767	38.0	35.0	41.0	32.0	41.0
19	36.92583744589915	38.0	35.0	41.0	32.0	41.0
20	36.77373515214761	38.0	35.0	41.0	32.0	41.0
21	36.6541752763769	38.0	35.0	41.0	32.0	41.0
22	36.53364626251959	38.0	35.0	40.0	31.0	41.0
23	36.47958823064738	38.0	35.0	40.0	31.0	41.0
24	36.36109561247781	38.0	35.0	40.0	31.0	41.0
25	36.26404751312764	37.0	35.0	40.0	31.0	41.0
26	36.11127376591843	37.0	35.0	40.0	31.0	41.0
27	36.0322235295585	37.0	35.0	40.0	31.0	41.0
28	36.00861705533359	37.0	35.0	40.0	31.0	41.0
29	36.047098614269736	37.0	35.0	40.0	31.0	41.0
30	36.031989040549135	37.0	35.0	40.0	31.0	41.0
31	35.920228834664464	37.0	35.0	40.0	30.0	41.0
32	35.756488984421445	37.0	35.0	40.0	30.0	41.0
33	35.6453370363749	37.0	35.0	40.0	30.0	41.0
34	35.52669391284825	37.0	35.0	40.0	29.0	41.0
35	35.43205680953589	37.0	35.0	40.0	29.0	41.0
36	35.26972929823759	37.0	34.0	40.0	28.0	41.0
37	35.1880132045584	37.0	34.0	40.0	27.0	41.0
38	35.15134436207764	37.0	34.0	40.0	27.0	41.0
39	35.102108738041686	37.0	34.0	40.0	27.0	41.0
40	34.9174440697979	36.0	34.0	40.0	26.0	41.0
41	34.88905551652025	36.0	34.0	40.0	26.0	41.0
42	34.828736544945805	36.0	34.0	40.0	26.0	41.0
43	34.7101603588846	36.0	34.0	40.0	26.0	41.0
44	34.541594358959436	36.0	34.0	40.0	25.0	41.0
45	34.42923005284318	35.0	34.0	40.0	24.0	41.0
46	34.432666730416635	35.0	34.0	40.0	24.0	41.0
47	34.42154845898313	35.0	34.0	40.0	25.0	41.0
48	34.33573837013508	35.0	34.0	40.0	24.0	41.0
49	34.305743317686876	36.0	34.0	40.0	24.0	41.0
50	34.176739854400616	35.0	34.0	40.0	24.0	41.0
51	33.78380196488485	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	2.0
10	4.0
11	1.0
12	5.0
13	5.0
14	22.0
15	47.0
16	167.0
17	466.0
18	1134.0
19	2158.0
20	3662.0
21	5936.0
22	9079.0
23	13415.0
24	19625.0
25	27615.0
26	36005.0
27	42284.0
28	45986.0
29	52340.0
30	61932.0
31	74215.0
32	92470.0
33	119246.0
34	211556.0
35	381697.0
36	163336.0
37	193824.0
38	287579.0
39	558913.0
40	503.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.96442751836623	24.655105748722576	29.382928035988243	13.997538696922957
2	30.806409366256034	26.465951281166454	29.195586284887515	13.532053067689992
3	27.96505947456168	26.177330234530586	31.589120375182418	14.268489915725317
4	24.607334849473855	29.409245685443803	30.688998557310526	15.294420907771814
5	23.136747837005192	31.963138660336014	30.24400992836444	14.656103574294349
6	21.277299884002776	40.086311911958525	27.90643722221991	10.729950981818787
7	85.84796464371391	5.345060555539387	6.950146139870199	1.8568286608765066
8	86.49721648241541	4.276430944234024	6.310623100493508	2.9157294728570657
9	81.16188472620081	6.317649455561422	9.321146002669185	3.1993198155685736
10	49.21038736420218	25.689518258129162	13.795021681918154	11.30507269575051
11	43.139616585524045	20.875217754643007	23.278272763935256	12.70689289589769
12	38.673058293801425	21.54313724674979	25.039850658772757	14.743953800676026
13	21.260835762068492	38.5300366285137	25.343023328330343	14.866104281087464
14	15.340113003746003	41.67085891993697	27.027061861027846	15.961966215289184
15	13.740016547274065	24.604424524889513	47.133288708356375	14.522270219480049
16	15.633432145782317	20.112671137479577	45.63160279890073	18.62229391783738
17	16.08079061046137	20.92386175126703	29.33973050394349	33.65561713432811
18	20.820004739671464	23.572340275150403	35.07535661870175	20.532298366476386
19	27.988591527629374	24.724205169567984	26.868698627574076	20.41850467522856
20	29.84820578489375	23.465074026184606	25.707063357766202	20.97965683115544
21	21.388806891648613	28.81578892663072	28.356830739679783	21.438573442040887
22	22.360855302819274	25.49880884572369	26.042249597751564	26.098086253705468
23	18.774046556878137	30.642724396419467	25.332504583761224	25.250724462941175
24	19.61999476141575	24.852841516195955	37.76507859955181	17.762085122836485
25	17.21265741737796	25.638047089051774	35.362273046652504	21.78702244691776
26	16.091434083226968	34.717137238434574	28.33766417348861	20.85376450484985
27	17.594658307105764	33.944612365553404	29.034021694390972	19.426707632949864
28	15.746519043916798	29.48025760530178	36.91002523667175	17.863198114109668
29	15.792003259563533	25.615180253031934	36.24800954586463	22.344806941539893
30	18.611193108351383	31.284575695463634	30.967433467901202	19.13679772828378
31	27.35285191021233	27.65061969125614	25.58067211867472	19.41585627985681
32	27.12971316672418	27.86864457868894	27.75738702743605	17.244255227150834
33	26.330538035863515	27.38453287211618	26.15309138834955	20.13183770367075
34	19.178789554429308	28.22175010290076	29.104409973266588	23.49505036940334
35	20.23382379231924	25.97057246084574	31.448510121693147	22.347093625141877
36	28.039480631789893	24.669906828037234	28.412334787109756	18.878277753063117
37	20.548180423493804	30.731738752634886	30.577325245402726	18.142755578468588
38	20.298973486943037	32.0735646902791	25.555352294790936	22.072109527986928
39	21.668530660269496	29.79511314926223	28.12824553161236	20.40811065885591
40	24.306532015649232	25.945834701878823	27.046394731480984	22.701238550990965
41	17.68176016430861	24.77442905668065	28.627407773892728	28.916403005118013
42	22.139878514736637	25.293256777938076	26.099624568128622	26.46724013919667
43	21.974156317691033	25.22419893315816	28.499769252836526	24.30187549631428
44	20.10073880668377	29.82122291839034	28.687318884264705	21.390719390661182
45	16.85285814662215	35.57298054655896	25.389921130203764	22.184240176615123
46	22.379938716879465	31.780162396111805	26.341223084694604	19.498675802314125
47	21.884310440165805	27.582351791720544	27.196733784295056	23.336603983818595
48	22.645983959953934	24.43404580850896	30.920868274551705	21.999101956985402
49	21.262332500426155	23.639153012393827	32.51676554840909	22.58174893877093
50	19.980916585939806	31.14226082328925	28.096730873970472	20.780091716800474
51	18.697130835720493	32.21899776736528	25.626987855631274	23.456883541282956
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3053.0
1	3704.0
2	4355.0
3	11811.0
4	19267.0
5	14321.5
6	9376.0
7	10326.5
8	11277.0
9	12260.5
10	13244.0
11	13458.5
12	13673.0
13	13670.5
14	13668.0
15	13336.5
16	13005.0
17	12317.5
18	11630.0
19	11529.0
20	11428.0
21	13039.0
22	14650.0
23	14208.5
24	13767.0
25	14286.0
26	18481.0
27	22157.0
28	24162.5
29	26168.0
30	30952.5
31	35737.0
32	39772.5
33	43808.0
34	52256.0
35	60704.0
36	62263.0
37	63822.0
38	79954.5
39	96087.0
40	133835.0
41	171583.0
42	202851.5
43	234120.0
44	242210.5
45	250301.0
46	247115.0
47	243929.0
48	232982.0
49	222035.0
50	199628.0
51	177221.0
52	161812.5
53	146404.0
54	126068.0
55	105732.0
56	92734.5
57	79737.0
58	73952.5
59	68168.0
60	61313.0
61	54458.0
62	48352.5
63	42247.0
64	36259.0
65	30271.0
66	26266.5
67	22262.0
68	17718.5
69	13175.0
70	11217.0
71	9259.0
72	8564.0
73	7869.0
74	6377.0
75	3774.0
76	2663.0
77	2313.0
78	1963.0
79	1278.5
80	594.0
81	501.5
82	409.0
83	281.0
84	153.0
85	108.0
86	63.0
87	40.5
88	18.0
89	22.5
90	27.0
91	14.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2405230.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.3538189073513
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.14521336818022	15.70469764726142
2	7.652111432241108	2.96195157836934
3	2.5466045781181683	1.4785957150059237
4	1.2304455481870198	0.9525528125987271
5	0.7827268617652606	0.7574376968262125
6	0.5062186679522821	0.587835865624144
7	0.39941251726067784	0.5411110269874738
8	0.31750505295112313	0.49159482375880187
9	0.25722160167888514	0.4480398268156792
>10	2.7293023156986465	12.374058683197198
>50	1.0892062418751232	15.411814943205258
>100	1.330160174821711	45.42091902051972
>500	0.011704195633908735	1.4164686385155705
>1k	0.00173395490872722	0.5370222853755541
>5k	0.0	0.0
>10k+	4.33488727181805E-4	0.915899435938904
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11100	0.46149432694586384	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	10734	0.44627748697629754	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03155623370737934	0.0
2	0.0	0.0	0.0	0.17075290096996962	0.0
3	0.0	0.0	0.0	0.2968115315375245	0.0
4	0.0	0.0	0.0	0.5828132860474882	0.0
5	0.0	0.0	0.0	0.9697201515031827	0.0
6	0.0	0.0	0.0	1.7333893224348607	0.0
7	0.0	0.0	0.0	2.1053703803794233	0.0
8	0.0	0.0	0.0	3.0893095462803974	0.0
9	0.0	0.0	0.0	3.6247261176685805	0.0
10	0.0	0.0	0.0	4.39525533940621	0.0
11	0.0	0.0	0.0	4.928593107519863	0.0
12	0.0	0.0	0.0	5.397529550188548	0.0
13	0.0	0.0	0.0	5.649522083127185	0.0
14	0.0	0.0	0.0	5.749969857352519	0.0
15	0.0	0.0	0.0	5.843308124378957	0.0
16	0.0	0.0	0.0	6.029568897776928	0.0
17	0.0	0.0	0.0	6.217409561663541	0.0
18	0.0	0.0	0.0	6.527068097437667	0.0
19	0.0	0.0	0.0	6.645559884085929	0.0
20	0.0	0.0	0.0	6.7902445919932815	0.0
21	0.0	0.0	0.0	6.953222768716505	0.0
22	0.0	0.0	0.0	7.116117793308748	0.0
23	0.0	0.0	0.0	7.300341339497678	0.0
24	0.0	0.0	0.0	7.440868440855968	0.0
25	0.0	0.0	0.0	7.551751807519447	0.0
26	0.0	0.0	0.0	7.672197669245769	0.0
27	0.0	0.0	0.0	7.790232119173634	0.0
28	0.0	0.0	0.0	7.902736952391248	0.0
29	0.0	0.0	0.0	8.027007812142706	0.0
30	0.0	0.0	0.0	8.223413145520388	0.0
31	0.0	0.0	0.0	8.372463340304254	0.0
32	0.0	0.0	0.0	8.50396843545108	0.0
33	0.0	0.0	0.0	8.633436303388866	0.0
34	0.0	0.0	0.0	8.769972102460056	0.0
35	0.0	0.0	0.0	8.940267666709628	0.0
36	0.0	0.0	0.0	9.08491079855149	0.0
37	0.0	0.0	0.0	9.229096593672955	0.0
38	0.0	0.0	0.0	9.374404942562666	0.0
39	0.0	0.0	0.0	9.514308402938596	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCGAA	20	7.034825E-4	45.000004	23
GTAATCG	20	7.034825E-4	45.000004	9
TACGCTA	30	2.1664946E-6	45.000004	10
CGTAGTC	40	6.8212103E-9	45.000004	11
CTACCGT	20	7.034825E-4	45.000004	29
CACGTAG	30	2.1664946E-6	45.000004	1
TAGTCGA	40	6.8212103E-9	45.000004	16
ACGACCG	20	7.034825E-4	45.000004	38
CGTGCAA	25	3.892082E-5	45.0	25
CAAATCG	25	3.892082E-5	45.0	19
GAACGTA	25	3.892082E-5	45.0	36
CAATCGA	35	1.2126657E-7	45.0	18
TTCGATC	35	1.2126657E-7	45.0	15
TTTAGCG	230	0.0	43.043476	1
CCAACGG	80	0.0	42.187504	2
CGTAAGG	385	0.0	42.077923	2
TAGTCCG	75	0.0	42.0	1
GTTCCGA	140	0.0	41.785713	9
TCTAGCG	65	0.0	41.53846	1
CGTTTTT	7725	0.0	41.213593	1
>>END_MODULE