FastQCFastQC Report
Thu 26 May 2016
SRR1546663_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546663_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2721480
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134110.4927833384775931No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC57520.21135558593118453No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG253.8922175E-545.043
CGTAGTC253.8922175E-545.021
ATATACG351.2127566E-745.01
TATAGCG2000.042.751
TAATGCG501.0822987E-940.51
CGTTTTT89450.040.0950281
ATAGCGG5800.039.568972
GGCGTAA800.039.3758
AGGGCGA28950.038.7823836
TACGGGA4950.038.6363644
TATACGA356.251441E-638.57142637
TACCGTT356.251441E-638.57142610
TAGGGCG13850.038.5018045
CACAACG4500.038.512
ACGGGTA3750.038.45
CGTTCGA950.037.89473714
TAGGGAC15850.037.6182985
CGTATTA301.140332E-437.50000441
CGGTACG301.140332E-437.50000437
ACGCGTA301.140332E-437.50000436