##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546663_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2721480
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32467885121331	31.0	31.0	33.0	30.0	34.0
2	31.72499595808163	31.0	31.0	34.0	30.0	34.0
3	31.844762041242266	31.0	31.0	34.0	30.0	34.0
4	35.6011313696959	37.0	35.0	37.0	33.0	37.0
5	35.50810698590473	37.0	35.0	37.0	33.0	37.0
6	35.574013037023974	37.0	35.0	37.0	33.0	37.0
7	35.92284014580302	37.0	35.0	37.0	35.0	37.0
8	35.889815835501274	37.0	35.0	37.0	35.0	37.0
9	37.60729162073578	39.0	37.0	39.0	35.0	39.0
10	37.080640680806034	39.0	37.0	39.0	33.0	39.0
11	36.82048260505313	39.0	37.0	39.0	32.0	39.0
12	36.18466569660626	38.0	35.0	39.0	32.0	39.0
13	35.948501183179744	38.0	35.0	39.0	31.0	39.0
14	36.94882894601467	39.0	35.0	41.0	31.0	41.0
15	37.162923482810825	39.0	35.0	41.0	32.0	41.0
16	37.26926635507151	39.0	35.0	41.0	32.0	41.0
17	37.19400583506034	39.0	35.0	41.0	32.0	41.0
18	37.11073570263239	39.0	35.0	41.0	32.0	41.0
19	37.03924408777577	38.0	35.0	41.0	32.0	41.0
20	36.883072078427915	38.0	35.0	41.0	32.0	41.0
21	36.77468656760292	38.0	35.0	41.0	31.0	41.0
22	36.66222496582742	38.0	35.0	41.0	31.0	41.0
23	36.5737539868013	38.0	35.0	40.0	31.0	41.0
24	36.46480077016917	38.0	35.0	40.0	31.0	41.0
25	36.35863610976381	38.0	35.0	40.0	31.0	41.0
26	36.1959466907712	38.0	35.0	40.0	31.0	41.0
27	36.09314527389509	38.0	35.0	40.0	30.0	41.0
28	36.006119831856196	38.0	35.0	40.0	30.0	41.0
29	36.04488366624043	38.0	35.0	40.0	30.0	41.0
30	36.01052515543013	38.0	35.0	40.0	30.0	41.0
31	35.929768728779926	38.0	35.0	40.0	30.0	41.0
32	35.81351948204653	38.0	35.0	40.0	30.0	41.0
33	35.66584542234372	38.0	35.0	40.0	29.0	41.0
34	35.51747431544601	38.0	34.0	40.0	29.0	41.0
35	35.38905962931934	38.0	34.0	40.0	28.0	41.0
36	35.26333539103723	37.0	34.0	40.0	27.0	41.0
37	35.19668930140953	37.0	34.0	40.0	27.0	41.0
38	35.122077693019975	37.0	34.0	40.0	27.0	41.0
39	35.02692689272014	37.0	34.0	40.0	26.0	41.0
40	34.8813612446169	37.0	34.0	40.0	26.0	41.0
41	34.83502652968238	37.0	34.0	40.0	25.0	41.0
42	34.76848626482649	37.0	34.0	40.0	25.0	41.0
43	34.64756823493099	36.0	34.0	40.0	25.0	41.0
44	34.478790584534885	36.0	34.0	40.0	24.0	41.0
45	34.381492055793174	36.0	34.0	40.0	24.0	41.0
46	34.358215750253535	36.0	34.0	40.0	24.0	41.0
47	34.307072622249656	35.0	33.0	40.0	24.0	41.0
48	34.21021796963417	35.0	33.0	40.0	23.0	41.0
49	34.15235533606714	36.0	33.0	40.0	24.0	41.0
50	34.022466819524674	35.0	33.0	40.0	24.0	41.0
51	33.64881535047107	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	3.0
11	3.0
12	8.0
13	25.0
14	45.0
15	112.0
16	235.0
17	624.0
18	1371.0
19	2588.0
20	4479.0
21	7323.0
22	11280.0
23	16146.0
24	23331.0
25	31833.0
26	40447.0
27	47414.0
28	53198.0
29	60602.0
30	70626.0
31	85823.0
32	105678.0
33	136601.0
34	230582.0
35	364779.0
36	204348.0
37	251473.0
38	357915.0
39	612156.0
40	431.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.427120537354675	23.849302585357968	27.381682025956465	13.341894851330894
2	31.93806311271808	26.220990049531874	28.027874538853858	13.81307229889619
3	29.29402383996943	26.49892705439687	30.354733453855992	13.852315651777708
4	25.88591501682908	28.59139144877052	30.059085497596893	15.463608036803503
5	23.727163161221103	32.18201125857989	28.930765612828313	15.160059967370696
6	21.753972103414316	41.005372076958125	26.612725428810798	10.627930390816761
7	85.03773681967165	4.741500948013581	8.159494098799183	2.061268133515587
8	85.07198289166189	4.628106765436454	7.252634595881653	3.047275747020004
9	79.29446477651865	5.879007010891132	10.44152446462954	4.385003747960668
10	46.59615356350221	23.192049914017375	16.236386084042508	13.975410438437908
11	39.44831488748769	23.94755794641151	21.954451254464484	14.649675911636315
12	34.59367696988404	21.728030336434585	26.365837705954114	17.312454987727264
13	23.207262224965827	32.55629289945177	27.693313932125168	16.54313094345724
14	18.318231256522186	35.37663330246777	29.263819686347137	17.041315754662904
15	17.399870658612226	23.9339256580978	42.44863089201464	16.217572791275337
16	19.37519290974029	21.03994885136029	40.483339947381566	19.10151829151785
17	19.74918059291268	21.754192571688936	29.391287093787206	29.105339741611182
18	23.099967664653057	23.034892778929112	32.9645266546144	20.90061290180343
19	28.05807869247615	25.100754001499187	26.58127195496568	20.259895351058983
20	30.101856342872264	24.254560018813294	25.434910416391084	20.208673221923366
21	24.405360318650146	28.16438114555315	27.63646986198686	19.79378867380984
22	23.588856063612447	24.932904155092082	26.65108690859385	24.827152872701618
23	21.413495597983452	29.1965033731646	25.66629187059982	23.723709158252127
24	21.731190381704074	24.686861560621427	34.43872451754192	19.143223540132574
25	21.073460029101813	25.081756985169832	32.3119773064656	21.532805679262754
26	19.076348163499272	30.896277025735998	28.56012169848759	21.46725311227714
27	20.335075032702793	30.301086173699606	27.99300380675221	21.370834986845395
28	19.251069271131886	28.40865999382689	33.45102664726546	18.88924408777577
29	19.591949968399547	25.65559915928098	32.61251230947867	22.139938562840808
30	21.45630318797125	28.9367917456678	29.6792554051472	19.92764966121375
31	27.26042447491806	26.48448638240957	25.302408983347295	20.952680159325073
32	29.206020253685494	26.62205858576951	25.565721592662815	18.606199567882182
33	26.95698663962256	27.319252759527906	24.42450431382924	21.2992562870203
34	22.62316827608507	27.43874656436939	27.57091729500125	22.367167864544292
35	21.701537398768316	26.804312359451476	29.838506988844305	21.655643252935903
36	28.52609609477196	26.25508914267237	25.80706086394168	19.41175389861399
37	22.226325381777563	30.088995693519706	28.550090391992594	19.134588532710143
38	23.052971177447564	30.437996972235698	24.91669973690786	21.59233211340888
39	22.061929538339434	28.678219204256507	27.48820494730808	21.771646310095978
40	24.689176477504887	25.30490762379294	27.050134485647515	22.955781413054662
41	19.011126298925586	24.413480900098474	28.672964710378178	27.902428090597763
42	22.659141349559796	24.870144186251597	26.8301438922939	25.640570571894706
43	22.885084586328027	24.357812660758114	27.825117215632673	24.931985537281186
44	21.201515351940856	28.049590663903462	28.268809618295926	22.480084365859753
45	19.828365448212	31.181746696650354	26.28272116642415	22.707166688713496
46	22.83430339374164	29.599629613298646	26.07595866954745	21.49010832341226
47	22.427980363625675	26.67923335831974	27.647272807443006	23.245513470611577
48	22.995612681335157	24.675286976204124	30.0918617810897	22.23723856137102
49	22.992930317327335	23.937600129341387	30.227045578141304	22.84242397518997
50	20.674926878022255	28.95064450225612	28.432176609785852	21.94225200993577
51	20.24119229242912	29.709349324632186	26.34007966253656	23.709378720402132
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3234.0
1	3912.5
2	4591.0
3	14336.5
4	24082.0
5	17490.5
6	10899.0
7	11428.0
8	11957.0
9	13075.0
10	14193.0
11	14679.0
12	15165.0
13	15101.0
14	15037.0
15	15182.0
16	15327.0
17	14599.5
18	13872.0
19	14051.0
20	14230.0
21	14248.5
22	14267.0
23	15439.0
24	16611.0
25	16664.0
26	19439.5
27	22162.0
28	24025.5
29	25889.0
30	31718.0
31	37547.0
32	44536.0
33	51525.0
34	57540.0
35	63555.0
36	68779.5
37	74004.0
38	87315.5
39	100627.0
40	128531.0
41	156435.0
42	178302.5
43	200170.0
44	212349.0
45	224528.0
46	232173.0
47	239818.0
48	236752.0
49	233686.0
50	217824.5
51	201963.0
52	183933.0
53	165903.0
54	154894.5
55	143886.0
56	131055.5
57	118225.0
58	108948.5
59	99672.0
60	95710.0
61	91748.0
62	84217.0
63	76686.0
64	67677.0
65	58668.0
66	50647.5
67	42627.0
68	38612.5
69	34598.0
70	28773.5
71	22949.0
72	20320.5
73	17692.0
74	14480.0
75	8741.0
76	6214.0
77	5465.0
78	4716.0
79	3389.5
80	2063.0
81	1688.0
82	1313.0
83	980.5
84	648.0
85	460.5
86	273.0
87	186.5
88	100.0
89	98.0
90	96.0
91	59.5
92	23.0
93	16.5
94	10.0
95	9.0
96	8.0
97	5.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2721480.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.40297746366272
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.52622851108279	16.429748255453767
2	7.252643165159778	2.959510301014848
3	2.428172347698721	1.48625837063958
4	1.2198535201014262	0.9955457551839615
5	0.8235992048849755	0.8401938008179346
6	0.5855431535300395	0.7168094259285238
7	0.4305537690475118	0.6149205192739998
8	0.3545614506226123	0.5787287429229376
9	0.30425574181180387	0.5586950739038595
>10	3.586490405371635	17.484975664741032
>50	1.4115190324271394	20.85182515354697
>100	1.0703609310936573	34.56437999495998
>500	0.003841003250801161	0.4742377658687112
>1k	0.002011954083752989	0.7290454039983587
>5k	1.829049167048172E-4	0.2146534174753562
>10k+	1.829049167048172E-4	0.5004723542701672
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13411	0.4927833384775931	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5752	0.21135558593118453	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.674471243588048E-5	0.0	0.0	0.0526919176330526	0.0
2	3.674471243588048E-5	0.0	0.0	0.1848626482649147	0.0
3	3.674471243588048E-5	0.0	0.0	0.28899716330819997	0.0
4	3.674471243588048E-5	0.0	0.0	0.5679997648338404	0.0
5	3.674471243588048E-5	0.0	0.0	1.0104428472742772	0.0
6	3.674471243588048E-5	0.0	0.0	1.7931419668709672	0.0
7	3.674471243588048E-5	0.0	0.0	2.188919264517836	0.0
8	3.674471243588048E-5	0.0	0.0	3.226479709569793	0.0
9	3.674471243588048E-5	0.0	0.0	3.757587783118009	0.0
10	3.674471243588048E-5	0.0	0.0	4.629980745770683	0.0
11	3.674471243588048E-5	0.0	0.0	5.150543086849802	0.0
12	3.674471243588048E-5	0.0	0.0	5.66728397783559	0.0
13	3.674471243588048E-5	0.0	0.0	5.911158634272528	0.0
14	3.674471243588048E-5	0.0	0.0	6.024405837999912	0.0
15	3.674471243588048E-5	0.0	0.0	6.1329864632479385	0.0
16	3.674471243588048E-5	0.0	0.0	6.316636536002469	0.0
17	3.674471243588048E-5	0.0	0.0	6.492459985008157	0.0
18	3.674471243588048E-5	0.0	0.0	6.77866455017123	0.0
19	3.674471243588048E-5	0.0	0.0	6.90907153460617	0.0
20	7.348942487176096E-5	0.0	0.0	7.041536222937519	0.0
21	7.348942487176096E-5	0.0	0.0	7.203029234093214	0.0
22	7.348942487176096E-5	0.0	0.0	7.37084233578788	0.0
23	7.348942487176096E-5	0.0	0.0	7.558130135073563	0.0
24	7.348942487176096E-5	0.0	0.0	7.704925261254905	0.0
25	7.348942487176096E-5	0.0	0.0	7.829416346987668	0.0
26	7.348942487176096E-5	0.0	0.0	7.947440363331717	0.0
27	7.348942487176096E-5	0.0	0.0	8.07531196260858	0.0
28	7.348942487176096E-5	0.0	0.0	8.200795155577113	0.0
29	7.348942487176096E-5	0.0	0.0	8.332929141496539	0.0
30	7.348942487176096E-5	0.0	0.0	8.541969810544263	0.0
31	7.348942487176096E-5	0.0	0.0	8.692035216132398	0.0
32	7.348942487176096E-5	0.0	0.0	8.849596543057453	0.0
33	7.348942487176096E-5	0.0	0.0	9.005798315622382	0.0
34	7.348942487176096E-5	0.0	0.0	9.156708849596543	0.0
35	7.348942487176096E-5	0.0	0.0	9.336574216970178	0.0
36	7.348942487176096E-5	0.0	0.0	9.499794229610359	0.0
37	7.348942487176096E-5	0.0	0.0	9.660589091229772	0.0
38	7.348942487176096E-5	0.0	0.0	9.83578788012405	0.0
39	7.348942487176096E-5	0.0	0.0	10.072240104648941	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCTCG	25	3.8922175E-5	45.0	43
CGTAGTC	25	3.8922175E-5	45.0	21
ATATACG	35	1.2127566E-7	45.0	1
TATAGCG	200	0.0	42.75	1
TAATGCG	50	1.0822987E-9	40.5	1
CGTTTTT	8945	0.0	40.095028	1
ATAGCGG	580	0.0	39.56897	2
GGCGTAA	80	0.0	39.375	8
AGGGCGA	2895	0.0	38.782383	6
TACGGGA	495	0.0	38.636364	4
TATACGA	35	6.251441E-6	38.571426	37
TACCGTT	35	6.251441E-6	38.571426	10
TAGGGCG	1385	0.0	38.501804	5
CACAACG	450	0.0	38.5	12
ACGGGTA	375	0.0	38.4	5
CGTTCGA	95	0.0	37.894737	14
TAGGGAC	1585	0.0	37.618298	5
CGTATTA	30	1.140332E-4	37.500004	41
CGGTACG	30	1.140332E-4	37.500004	37
ACGCGTA	30	1.140332E-4	37.500004	36
>>END_MODULE