FastQCFastQC Report
Thu 26 May 2016
SRR1546662_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546662_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3442705
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT391971.1385523883109359No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTC48750.14160376796733964No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC47510.13800194904878577No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG1900.043.815791
CGTTTTT245300.042.450061
AGGGCGA39650.037.9066856
TAATGCG950.037.8947371
GGGCGAT77350.037.5242427
CGTAAGG4450.037.415732
CACGACC12750.037.41176627
TGGGCGA23650.037.293876
CGGTCTA1750.037.28571331
TTTAGCG3150.037.1428571
TGATTCG4550.037.0879115
TAGGGAC25250.037.0693055
AGGGTAC17850.036.806726
AACACGT11750.036.57446741
ACACGAC13000.036.5192326
GACACGA13100.036.41221225
GACCGAT22250.036.3033689
GCGAGAC13150.036.2737621
AAGGGCG17500.036.128575
TAACGCG250.002107691736.01
Produced by FastQC (version 0.11.5)