##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546662_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3442705
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.340037557676304	31.0	31.0	33.0	30.0	34.0
2	31.74156571649328	31.0	31.0	34.0	30.0	34.0
3	31.762884417921374	31.0	31.0	34.0	30.0	34.0
4	35.59216517244434	37.0	35.0	37.0	33.0	37.0
5	35.52212518934965	37.0	35.0	37.0	33.0	37.0
6	35.59504139913237	37.0	35.0	37.0	33.0	37.0
7	35.95012003642485	37.0	35.0	37.0	35.0	37.0
8	35.91944938645629	37.0	35.0	37.0	35.0	37.0
9	37.63126233586671	39.0	37.0	39.0	35.0	39.0
10	37.10212667074292	39.0	37.0	39.0	33.0	39.0
11	36.872047416203245	39.0	37.0	39.0	32.0	39.0
12	36.48689097671743	39.0	35.0	39.0	32.0	39.0
13	36.369464708710154	39.0	35.0	39.0	32.0	39.0
14	37.45021313182512	40.0	36.0	41.0	32.0	41.0
15	37.58876842482873	40.0	35.0	41.0	32.0	41.0
16	37.62495886228997	40.0	35.0	41.0	33.0	41.0
17	37.536431672188	39.0	35.0	41.0	32.0	41.0
18	37.38400792400162	39.0	36.0	41.0	32.0	41.0
19	37.26383933563869	39.0	36.0	41.0	32.0	41.0
20	37.06642799775177	39.0	35.0	41.0	32.0	41.0
21	36.98450927395754	39.0	35.0	41.0	32.0	41.0
22	36.879500857610516	38.0	35.0	41.0	31.0	41.0
23	36.782996800481015	38.0	35.0	41.0	31.0	41.0
24	36.6609018199352	38.0	35.0	41.0	31.0	41.0
25	36.5544979892265	38.0	35.0	40.0	31.0	41.0
26	36.400487116961806	38.0	35.0	40.0	31.0	41.0
27	36.28636087030402	38.0	35.0	40.0	30.0	41.0
28	36.18826010361039	38.0	35.0	40.0	30.0	41.0
29	36.188527044867335	38.0	35.0	40.0	30.0	41.0
30	36.13730540374502	38.0	35.0	40.0	30.0	41.0
31	36.02081967522631	38.0	35.0	40.0	30.0	41.0
32	35.81228568814348	38.0	35.0	40.0	29.0	41.0
33	35.549641633541064	38.0	35.0	40.0	27.0	41.0
34	35.280266534599974	38.0	34.0	40.0	25.0	41.0
35	35.061356694808296	38.0	34.0	40.0	24.0	41.0
36	34.88716111313633	38.0	34.0	40.0	24.0	41.0
37	34.825579014176355	38.0	34.0	40.0	23.0	41.0
38	34.72729321855925	38.0	34.0	40.0	23.0	41.0
39	34.6145545435929	38.0	34.0	40.0	23.0	41.0
40	34.46566638733206	37.0	34.0	40.0	23.0	41.0
41	34.38342989015904	37.0	34.0	40.0	22.0	41.0
42	34.313085495271885	37.0	33.0	40.0	22.0	41.0
43	34.18991258327391	37.0	33.0	40.0	22.0	41.0
44	34.05230799618323	37.0	33.0	40.0	21.0	41.0
45	33.926885980646034	36.0	33.0	40.0	21.0	41.0
46	33.87212177633576	36.0	33.0	40.0	21.0	41.0
47	33.79805153215277	36.0	33.0	40.0	21.0	41.0
48	33.69471418550239	36.0	33.0	40.0	20.0	41.0
49	33.582897459991486	36.0	33.0	40.0	20.0	41.0
50	33.46466891586703	35.0	33.0	40.0	20.0	41.0
51	33.10705244858331	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	5.0
11	7.0
12	11.0
13	15.0
14	37.0
15	112.0
16	303.0
17	803.0
18	1784.0
19	3487.0
20	6327.0
21	10451.0
22	16033.0
23	23185.0
24	33917.0
25	50488.0
26	68224.0
27	76294.0
28	76685.0
29	79417.0
30	87920.0
31	104324.0
32	125174.0
33	158935.0
34	249310.0
35	371762.0
36	272002.0
37	334511.0
38	481212.0
39	809426.0
40	541.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.47289413411838	22.511891085643413	26.51174004162425	17.503474738613967
2	35.12305004349777	25.347393982348184	26.291390055203685	13.23816591895036
3	27.756894651153672	25.369789162882096	33.2351159916403	13.638200194323941
4	24.26304315937613	27.130003877764725	33.49552169006639	15.11143127279276
5	22.531526808134885	30.579035961547678	32.056740266737926	14.832696963579512
6	20.675631516496477	39.18421706187431	29.63181567982154	10.508335741807677
7	82.83422483192722	4.043826003099308	11.221612075388395	1.900337089585079
8	82.65671906248139	4.017683769013029	10.792298497838182	2.5332986706673966
9	77.13690252287083	5.595977581581925	12.981594414856923	4.285525480690329
10	39.897783864722655	26.36949724126813	19.983298017111544	13.749420876897672
11	32.3418939467657	25.44034414798828	26.678033697339735	15.539728207906284
12	28.715733703584828	22.514795778319666	30.80731575897441	17.9621547591211
13	22.582881774651035	29.041843550347764	31.638377380577193	16.736897294424008
14	19.161763787486873	31.551091365655786	32.338146893213334	16.94899795364401
15	18.047523676876175	23.995985714721417	40.80581984224614	17.150670766156264
16	20.306590311978518	22.167133111898927	38.75176641623375	18.77451015988881
17	20.504283695524304	22.744876485205673	31.925070547723372	24.825769271546648
18	22.732734869818934	23.098784240880356	34.26799566038914	19.90048522891157
19	26.043765004553105	25.080946523155482	29.11289233320892	19.762396139082494
20	27.51870985170092	24.351345816734224	28.619878845268477	19.510065486296384
21	24.017741862866554	26.474908538489355	30.64389774900841	18.86345184963568
22	23.364418386123702	23.57567668446759	30.048900501204724	23.011004428203986
23	21.564496522356695	27.382160248990257	29.058487439382695	21.994855789270357
24	21.04011235351272	24.94872491253244	34.87952060952071	19.13164212443413
25	21.016468155128017	26.06435927562774	32.5225948781554	20.396577691088837
26	20.031835431731736	28.48890625249622	30.469993798481138	21.00926451729091
27	20.424404646927343	27.823063550318714	30.792211357057898	20.96032044569604
28	19.12188235704192	27.376902755246242	34.377357339650075	19.12385754806177
29	20.54660506781731	25.356137107303706	33.562300574693445	20.53495725018554
30	21.51006258160371	27.12814487445192	31.944241519386647	19.417551024557724
31	25.226355438528714	26.440168414081366	28.384802066979308	19.94867408041061
32	26.512524308646835	25.589616304620932	28.65284710714395	19.245012279588288
33	24.797158048685557	26.809761510207814	27.561205505554497	20.83187493555213
34	22.08054422321982	27.121754550564166	29.711084742956483	21.08661648325953
35	22.539340431434006	27.22280880877101	29.739928341231675	20.49792241856331
36	25.657150409343814	27.96379591048318	26.98906818911292	19.389985491060084
37	22.84282272224893	29.547056747528472	28.504998249922664	19.105122280299938
38	22.37589337454124	29.46514441405813	27.1104843429803	21.048477868420328
39	22.720186597457523	28.99644320381793	27.29098194588267	20.992388252841877
40	24.074935261661977	26.302166465032585	28.564166839737936	21.0587314335675
41	19.995613914058858	26.614972819338284	29.032345205296416	24.357068061306446
42	23.033951500346383	26.958685103719315	27.544590663446332	22.46277273248797
43	22.40055421536263	26.233760952506824	28.642796870484112	22.72288796164644
44	22.292993445560978	27.675069458463618	28.857017955357776	21.174919140617625
45	20.819297616263952	30.026069616769373	27.397874636368787	21.756758130597888
46	22.61047635507544	28.72299543527546	27.381492169674722	21.28503603997438
47	22.060734219167777	28.13752557944988	28.827215808499425	20.974524392882923
48	22.75629192742335	26.31395951729817	29.541131174468916	21.388617380809567
49	22.23809475397979	25.93512949846124	29.76110355084156	22.065672196717408
50	21.135705789488206	28.87401040751386	28.923709699204547	21.066574103793386
51	21.269089277181752	28.91743556302384	27.725262547909274	22.08821261188513
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3758.0
1	4728.5
2	5699.0
3	34074.5
4	62450.0
5	43234.0
6	24018.0
7	24573.5
8	25129.0
9	26977.0
10	28825.0
11	29619.5
12	30414.0
13	29968.5
14	29523.0
15	28789.5
16	28056.0
17	27086.5
18	26117.0
19	24481.0
20	22845.0
21	23324.0
22	23803.0
23	24078.0
24	24353.0
25	25505.0
26	30223.0
27	33789.0
28	38495.5
29	43202.0
30	50081.0
31	56960.0
32	66449.5
33	75939.0
34	80058.5
35	84178.0
36	97215.5
37	110253.0
38	118410.5
39	126568.0
40	149727.0
41	172886.0
42	195639.0
43	218392.0
44	224024.0
45	229656.0
46	238397.0
47	247138.0
48	256377.0
49	265616.0
50	262702.5
51	259789.0
52	248600.0
53	237411.0
54	215667.5
55	193924.0
56	176106.0
57	158288.0
58	142913.0
59	127538.0
60	118589.0
61	109640.0
62	99033.0
63	88426.0
64	80086.0
65	71746.0
66	63127.0
67	54508.0
68	46281.0
69	38054.0
70	32639.5
71	27225.0
72	23107.0
73	18989.0
74	16325.0
75	11017.5
76	8374.0
77	6435.0
78	4496.0
79	3473.0
80	2450.0
81	1778.0
82	1106.0
83	867.0
84	628.0
85	374.0
86	120.0
87	95.5
88	71.0
89	41.5
90	12.0
91	11.5
92	11.0
93	9.0
94	7.0
95	6.0
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3442705.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.057812861881246
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.89235140415344	18.421428877726253
2	6.693037502921741	3.0865361243984495
3	2.4821790524013347	1.717008602398551
4	1.4347608436608623	1.323297881387481
5	0.9676328319263208	1.1155748378784647
6	0.7247823418530993	1.0027137362426881
7	0.525298183998093	0.8478559056319864
8	0.42487929688023485	0.7837429853081713
9	0.35495970880804717	0.7366135085283431
>10	4.434641362131098	24.254378899872084
>50	1.4126789502306838	23.110375852888552
>100	0.6475455872716682	20.98014934914115
>500	0.0030748880565972846	0.5141150272485661
>1k	0.0020499253710648557	0.9482605447136537
>5k	0.0	0.0
>10k+	1.2812033569155348E-4	1.1579478666357546
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39197	1.1385523883109359	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTC	4875	0.14160376796733964	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4751	0.13800194904878577	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.714078028759362E-5	0.0	0.0	0.02771076813145477	0.0
2	8.714078028759362E-5	0.0	0.0	0.13039165423700258	0.0
3	8.714078028759362E-5	0.0	0.0	0.2472183936759031	0.0
4	8.714078028759362E-5	0.0	0.0	0.4343096489533666	0.0
5	8.714078028759362E-5	0.0	0.0	0.8294349937040786	0.0
6	8.714078028759362E-5	0.0	0.0	1.4161538673804466	0.0
7	8.714078028759362E-5	0.0	0.0	1.7380809566895798	0.0
8	8.714078028759362E-5	0.0	0.0	2.411533953678866	0.0
9	8.714078028759362E-5	0.0	0.0	2.810987290517195	0.0
10	8.714078028759362E-5	0.0	0.0	3.48568930535727	0.0
11	8.714078028759362E-5	0.0	0.0	3.8716067743242597	0.0
12	8.714078028759362E-5	0.0	0.0	4.260428935967503	0.0
13	8.714078028759362E-5	0.0	0.0	4.464512643401046	0.0
14	8.714078028759362E-5	0.0	0.0	4.561703660348476	0.0
15	8.714078028759362E-5	0.0	0.0	4.656512829301378	0.0
16	8.714078028759362E-5	0.0	0.0	4.796286640882678	0.0
17	8.714078028759362E-5	0.0	0.0	4.938442300458505	0.0
18	8.714078028759362E-5	0.0	0.0	5.166344487837326	0.0
19	8.714078028759362E-5	0.0	0.0	5.276751856461707	0.0
20	8.714078028759362E-5	0.0	0.0	5.393752877461182	0.0
21	8.714078028759362E-5	0.0	0.0	5.522750279213583	0.0
22	8.714078028759362E-5	0.0	0.0	5.664005484059773	0.0
23	8.714078028759362E-5	0.0	0.0	5.815601394833423	0.0
24	8.714078028759362E-5	0.0	0.0	5.938324660405118	0.0
25	8.714078028759362E-5	0.0	0.0	6.04530449167152	0.0
26	8.714078028759362E-5	0.0	0.0	6.153271918447849	0.0
27	8.714078028759362E-5	0.0	0.0	6.2726547874418515	0.0
28	8.714078028759362E-5	0.0	0.0	6.385908754888961	0.0
29	8.714078028759362E-5	0.0	0.0	6.514557593520212	0.0
30	8.714078028759362E-5	0.0	0.0	6.706325404006442	0.0
31	8.714078028759362E-5	0.0	0.0	6.8403479240887615	0.0
32	8.714078028759362E-5	0.0	0.0	6.976287541337408	0.0
33	8.714078028759362E-5	0.0	0.0	7.1148994758482065	0.0
34	8.714078028759362E-5	0.0	0.0	7.256648478449359	0.0
35	8.714078028759362E-5	0.0	0.0	7.406937277518695	0.0
36	8.714078028759362E-5	0.0	0.0	7.541918346184178	0.0
37	8.714078028759362E-5	0.0	0.0	7.685991102926333	0.0
38	8.714078028759362E-5	0.0	0.0	7.837441779066171	0.0
39	8.714078028759362E-5	0.0	0.0	8.032927596178006	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGCG	190	0.0	43.81579	1
CGTTTTT	24530	0.0	42.45006	1
AGGGCGA	3965	0.0	37.906685	6
TAATGCG	95	0.0	37.894737	1
GGGCGAT	7735	0.0	37.524242	7
CGTAAGG	445	0.0	37.41573	2
CACGACC	1275	0.0	37.411766	27
TGGGCGA	2365	0.0	37.29387	6
CGGTCTA	175	0.0	37.285713	31
TTTAGCG	315	0.0	37.142857	1
TGATTCG	455	0.0	37.08791	15
TAGGGAC	2525	0.0	37.069305	5
AGGGTAC	1785	0.0	36.80672	6
AACACGT	1175	0.0	36.574467	41
ACACGAC	1300	0.0	36.51923	26
GACACGA	1310	0.0	36.412212	25
GACCGAT	2225	0.0	36.303368	9
GCGAGAC	1315	0.0	36.27376	21
AAGGGCG	1750	0.0	36.12857	5
TAACGCG	25	0.0021076917	36.0	1
>>END_MODULE