FastQCFastQC Report
Thu 26 May 2016
SRR1546661_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546661_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences352414
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39291.1148819286407465No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC5870.16656545994200003No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGG302.1612013E-645.00000445
CGGGATA302.1612013E-645.0000046
TCGTTTG253.8852828E-545.01
CGACCCC551.8189894E-1245.020
ATTCGGC253.8852828E-545.017
TACGGGT207.026623E-445.04
TGTTGCG207.026623E-445.01
ACTACGG207.026623E-445.02
CATACGA351.2087185E-745.018
GCTACGA253.8852828E-545.010
AGCTACG253.8852828E-545.09
CGTTTGG406.7921064E-945.02
TGATGCG253.8852828E-545.01
CGTTTTT32000.043.4531251
TGGGCGA1500.042.0000046
ACGGGAT700.041.7857135
ATGGGCG650.041.538465
TTACGGG1150.041.0869563
CTCGTCC556.002665E-1140.90909237
TACGGGA950.040.2631574
Produced by FastQC (version 0.11.5)