##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546661_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	352414
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28635922522942	31.0	31.0	33.0	30.0	34.0
2	31.671133950410596	31.0	31.0	34.0	30.0	34.0
3	31.650147269972248	31.0	31.0	34.0	30.0	34.0
4	35.510328761059434	37.0	35.0	37.0	33.0	37.0
5	35.45623896893994	37.0	35.0	37.0	33.0	37.0
6	35.53074225201042	37.0	35.0	37.0	33.0	37.0
7	35.95593818633766	37.0	35.0	37.0	35.0	37.0
8	35.94993104700721	37.0	35.0	37.0	35.0	37.0
9	37.67772846708701	39.0	37.0	39.0	35.0	39.0
10	37.05322149517329	39.0	37.0	39.0	33.0	39.0
11	36.80789923215309	39.0	37.0	39.0	32.0	39.0
12	36.28081177251755	38.0	35.0	39.0	32.0	39.0
13	36.136853246465805	38.0	35.0	39.0	32.0	39.0
14	37.21629957947187	39.0	35.0	41.0	32.0	41.0
15	37.41243253673237	39.0	35.0	41.0	32.0	41.0
16	37.44821999126028	39.0	35.0	41.0	32.0	41.0
17	37.390515132769984	39.0	35.0	41.0	32.0	41.0
18	37.2053720907796	39.0	35.0	41.0	32.0	41.0
19	37.074401130488575	38.0	35.0	41.0	32.0	41.0
20	36.83887700261624	38.0	35.0	41.0	32.0	41.0
21	36.74106874301248	38.0	35.0	41.0	32.0	41.0
22	36.64220490672902	38.0	35.0	40.0	31.0	41.0
23	36.56520172297355	38.0	35.0	40.0	31.0	41.0
24	36.403891445856296	38.0	35.0	40.0	31.0	41.0
25	36.29450589363646	38.0	35.0	40.0	31.0	41.0
26	36.12081245353476	38.0	35.0	40.0	31.0	41.0
27	36.03545829620844	37.0	35.0	40.0	30.0	41.0
28	35.97100852974059	37.0	35.0	40.0	30.0	41.0
29	35.98347965744834	37.0	35.0	40.0	30.0	41.0
30	35.904362482761755	37.0	35.0	40.0	30.0	41.0
31	35.75646824473488	37.0	35.0	40.0	30.0	41.0
32	35.48675705278451	37.0	34.0	40.0	29.0	41.0
33	35.1775837509293	38.0	34.0	40.0	26.0	41.0
34	34.87287678696079	37.0	34.0	40.0	24.0	41.0
35	34.60590952686329	37.0	34.0	40.0	23.0	41.0
36	34.41619231926087	37.0	34.0	40.0	22.0	41.0
37	34.34464294835052	37.0	34.0	40.0	22.0	41.0
38	34.270051700556735	37.0	33.0	40.0	22.0	41.0
39	34.17732836947454	37.0	33.0	40.0	21.0	41.0
40	34.06099644168506	37.0	33.0	40.0	20.0	41.0
41	34.023906541737844	37.0	33.0	40.0	20.0	41.0
42	33.936520683060266	37.0	33.0	40.0	19.0	41.0
43	33.82334129745129	37.0	33.0	40.0	18.0	41.0
44	33.698936478119485	36.0	33.0	40.0	18.0	41.0
45	33.59034544598115	36.0	33.0	40.0	18.0	41.0
46	33.56014800774089	36.0	33.0	40.0	18.0	41.0
47	33.54089792119496	36.0	33.0	40.0	18.0	41.0
48	33.43141021639322	36.0	33.0	40.0	18.0	41.0
49	33.35234979314102	36.0	33.0	40.0	18.0	41.0
50	33.23289369888824	35.0	33.0	40.0	18.0	41.0
51	32.87938333891389	35.0	32.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	1.0
14	4.0
15	6.0
16	32.0
17	78.0
18	188.0
19	390.0
20	698.0
21	1102.0
22	1733.0
23	2694.0
24	3975.0
25	6232.0
26	8230.0
27	8932.0
28	8292.0
29	8166.0
30	9158.0
31	10787.0
32	13062.0
33	16461.0
34	26839.0
35	43060.0
36	26229.0
37	31054.0
38	44997.0
39	79958.0
40	54.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.579051910537036	23.459624192001453	26.58520944116863	18.376114456292882
2	34.72279761870981	24.348067897416108	28.228163466831624	12.700971017042455
3	26.68594323721532	23.776297195911628	36.085115801301875	13.452643765571176
4	22.843587371670818	27.137684654979655	36.14810989347756	13.870618079871969
5	21.092805620662062	31.259541334907237	34.654128383094886	12.993524661335815
6	18.758902881270323	39.61108242010817	31.929492017910754	9.700522680710755
7	83.21150692083742	3.7262991822118305	11.66497358220729	1.3972203147434552
8	83.77022479243162	2.947953259518634	11.165844716725214	2.1159772313245218
9	78.42055082942221	5.126640825846874	13.675109388389792	2.7776989563411214
10	41.14592496325344	25.7064134796007	21.115222437247102	12.032439119898756
11	35.456593665404895	23.354350281203356	27.150169970546006	14.038886082845744
12	30.196303211563674	22.190662118985056	31.226909260131553	16.38612540931972
13	20.50854960359123	33.5940115886429	31.410216393219336	14.487222414546528
14	16.994217028835404	35.701192347636585	32.37754459243957	14.927046031088437
15	16.228072664536597	24.829320061064543	44.73204810251579	14.21055917188307
16	17.775400523248226	21.959400023835602	43.11803730839297	17.147162144523204
17	17.91330650882201	22.940064810138075	32.23623352080224	26.910395160237677
18	20.61297224287344	24.645729170804792	35.84789480554121	18.89340378078056
19	25.026815052750457	26.653594919611596	30.040236766984286	18.279353260653664
20	26.3862956636229	24.703331876713182	30.0666261839768	18.843746275687117
21	21.330594130766656	28.15495411646529	31.83840596571078	18.67604578705727
22	21.95429239474028	25.176922596718633	30.236596729982352	22.63218827855874
23	19.380047330696286	29.063544581089285	29.616871066416206	21.939537021798223
24	19.756025583546624	26.05685358697441	37.49255137423598	16.69456945524298
25	18.72116317739931	27.333193346461833	34.749754550046255	19.195888926092607
26	18.08412832634345	31.909912773045342	30.930666772602677	19.075292128008535
27	18.200468766848083	30.837026905855048	32.270568138609704	18.69193618868717
28	17.46071381954179	28.209151736310133	37.48318738756122	16.846947056586856
29	17.790439653362238	25.673213890481083	36.33964598455226	20.19670047160442
30	19.525614759913058	28.669405869233344	33.740714046547524	18.06426532430607
31	25.01461349435607	26.686226994387287	29.441225376971403	18.857934134285244
32	25.82161889141748	26.563643896099475	29.851254490457247	17.7634827220258
33	23.776864710255552	27.887371103304638	28.692673957334268	19.64309022910554
34	20.36014460265483	27.62092311883183	30.36826005777296	21.65067222074038
35	19.898187926699848	27.65242016491967	31.690000964774384	20.7593909436061
36	25.536159176423183	27.594817459011278	27.905247805138274	18.963775559427265
37	20.40696453602865	30.799854716327957	30.795598358748517	17.997582388894877
38	19.93082000147554	32.22545074826766	27.63482722025799	20.208902029998807
39	20.345389229712783	31.645451088776266	28.499435323227797	19.509724358283158
40	21.967345224650554	28.576049759657675	27.933907279506485	21.522697736185282
41	18.01999920547992	27.22167677788056	28.97926870101642	25.7790553156231
42	21.708558683820733	28.51674451071751	27.04007218782455	22.734624617637213
43	21.635633090626367	28.165453131827906	28.252566583620403	21.946347193925327
44	20.130585050537153	31.445402282542688	28.21936699450079	20.204645672419367
45	19.225115914804746	33.80881576781854	25.693928164034347	21.272140153342374
46	22.168812816743944	30.644639543264457	26.907273831346085	20.279273808645513
47	21.020447541811617	28.759924407089386	28.17481711850267	22.04481093259632
48	21.86235507102442	26.85108991129751	30.14664570647023	21.13990931120784
49	21.377981578484395	26.26030747927154	30.144943163438455	22.21676777880561
50	19.809088174703614	31.058925014329734	28.63081489384644	20.501171917120207
51	19.049186468187983	31.28536323755583	27.029856929633894	22.63559336462229
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	401.0
1	407.5
2	414.0
3	3163.5
4	5913.0
5	4225.0
6	2537.0
7	2707.0
8	2877.0
9	3167.0
10	3457.0
11	3749.5
12	4042.0
13	4072.5
14	4103.0
15	4023.5
16	3944.0
17	3689.5
18	3435.0
19	3221.5
20	3008.0
21	2917.5
22	2827.0
23	2729.0
24	2631.0
25	2702.0
26	3106.0
27	3439.0
28	3816.0
29	4193.0
30	4876.0
31	5559.0
32	6364.0
33	7169.0
34	8180.0
35	9191.0
36	10326.0
37	11461.0
38	12977.5
39	14494.0
40	18049.0
41	21604.0
42	25255.5
43	28907.0
44	29898.5
45	30890.0
46	31875.5
47	32861.0
48	31407.5
49	29954.0
50	28040.5
51	26127.0
52	23773.0
53	21419.0
54	18611.5
55	15804.0
56	13986.5
57	12169.0
58	10840.0
59	9511.0
60	8470.0
61	7429.0
62	6388.5
63	5348.0
64	4703.0
65	4058.0
66	3490.0
67	2922.0
68	2461.5
69	2001.0
70	1575.0
71	1149.0
72	1053.0
73	957.0
74	783.0
75	462.0
76	315.0
77	285.0
78	255.0
79	195.0
80	135.0
81	107.0
82	79.0
83	48.5
84	18.0
85	13.5
86	9.0
87	8.5
88	8.0
89	5.0
90	2.0
91	3.0
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	352414.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.308548782531226
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.21570722123859	31.399588693998275
2	8.315472319167332	7.036311325305585
3	4.001461077780974	5.07888033632089
4	2.584832310344289	4.374420155874569
5	1.8450426672739082	3.9030538847104843
6	1.394922747797586	3.5410294273834007
7	1.1328480425566776	3.3550409670192938
8	0.9048831001526807	3.062743262823822
9	0.7783915854951375	2.9639356530149565
>10	4.787365148923365	32.609165575128
>50	0.02829480567788885	0.7499052641845944
>100	0.009431601892629617	0.6387448487410399
>500	6.736858494735441E-4	0.16731067657786275
>1k	6.736858494735441E-4	1.119869928917245
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3929	1.1148819286407465	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	587	0.16656545994200003	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05930524894016696	0.0
2	0.0	0.0	0.0	0.1847259189476014	0.0
3	0.0	0.0	0.0	0.2894323154017718	0.0
4	0.0	0.0	0.0	0.4613891616110597	0.0
5	0.0	0.0	0.0	0.7471326337773188	0.0
6	0.0	0.0	0.0	1.414529502233169	0.0
7	0.0	0.0	0.0	1.7510655081807194	0.0
8	0.0	0.0	0.0	2.696828162331803	0.0
9	0.0	0.0	0.0	3.09266941721952	0.0
10	0.0	0.0	0.0	3.6091074701913093	0.0
11	0.0	0.0	0.0	3.9467785048267094	0.0
12	0.0	0.0	0.0	4.257208850953708	0.0
13	0.0	0.0	0.0	4.433705811914396	0.0
14	0.0	0.0	0.0	4.530183250381653	0.0
15	0.0	0.0	0.0	4.605095143779759	0.0
16	0.0	0.0	0.0	4.7398798004619564	0.0
17	0.0	0.0	0.0	4.895662487869381	0.0
18	0.0	0.0	0.0	5.159272900622563	0.0
19	0.0	0.0	0.0	5.273059526579535	0.0
20	0.0	0.0	0.0	5.387981181224355	0.0
21	0.0	0.0	0.0	5.526738438314029	0.0
22	0.0	0.0	0.0	5.685074940269115	0.0
23	0.0	0.0	0.0	5.871787159420454	0.0
24	0.0	0.0	0.0	6.007139330446577	0.0
25	0.0	0.0	0.0	6.134830057829712	0.0
26	0.0	0.0	0.0	6.257696913289483	0.0
27	0.0	0.0	0.0	6.389360241080094	0.0
28	0.0	0.0	0.0	6.524996169278179	0.0
29	0.0	0.0	0.0	6.694115443767841	0.0
30	0.0	0.0	0.0	6.9364440686238344	0.0
31	0.0	0.0	0.0	7.095348084922846	0.0
32	0.0	0.0	0.0	7.2585084588012965	0.0
33	0.0	0.0	0.0	7.419966289647971	0.0
34	0.0	0.0	0.0	7.604975965767535	0.0
35	0.0	0.0	0.0	7.800484657249712	0.0
36	0.0	0.0	0.0	7.9781166468982505	0.0
37	0.0	0.0	0.0	8.167950194941177	0.0
38	0.0	0.0	0.0	8.352108599544854	0.0
39	0.0	0.0	0.0	8.520092845346666	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTCGG	30	2.1612013E-6	45.000004	45
CGGGATA	30	2.1612013E-6	45.000004	6
TCGTTTG	25	3.8852828E-5	45.0	1
CGACCCC	55	1.8189894E-12	45.0	20
ATTCGGC	25	3.8852828E-5	45.0	17
TACGGGT	20	7.026623E-4	45.0	4
TGTTGCG	20	7.026623E-4	45.0	1
ACTACGG	20	7.026623E-4	45.0	2
CATACGA	35	1.2087185E-7	45.0	18
GCTACGA	25	3.8852828E-5	45.0	10
AGCTACG	25	3.8852828E-5	45.0	9
CGTTTGG	40	6.7921064E-9	45.0	2
TGATGCG	25	3.8852828E-5	45.0	1
CGTTTTT	3200	0.0	43.453125	1
TGGGCGA	150	0.0	42.000004	6
ACGGGAT	70	0.0	41.785713	5
ATGGGCG	65	0.0	41.53846	5
TTACGGG	115	0.0	41.086956	3
CTCGTCC	55	6.002665E-11	40.909092	37
TACGGGA	95	0.0	40.263157	4
>>END_MODULE