FastQCFastQC Report
Thu 26 May 2016
SRR1546660_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546660_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2247109
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141230.6284964369774675No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC44040.1959851524781397No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG850.042.3529431
TAGTCGT650.041.53846418
CGTTTTT90350.040.3182071
CGACGGT1550.039.1935528
TCGTTAG1150.039.1304321
CGTTAGG2850.038.684212
AGGGTAC9700.038.2731976
CGGTCTA1650.038.18181631
GACCGAT15400.038.1331149
TAAGGGA23000.038.0543484
GGACCGA16400.037.1798828
TAGGGAT25450.036.954815
GCGATAT2750.036.8181849
TAGGGAC21350.036.779865
GGGCGAT52300.036.5248577
ACGGGAC6600.036.1363645
AACGGGA6600.036.1363644
GGCGATA11650.036.115888
TGGGCGA16950.036.1061946
CGCATTA250.002107536236.043
Produced by FastQC (version 0.11.5)