FastQCFastQC Report
Thu 26 May 2016
SRR1546659_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546659_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2004162
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107950.5386291128162295No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC32750.16340994390673008No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTAAG207.034545E-445.01
CGTTTTT70750.040.929331
GTTTACG501.0822987E-940.51
TCGATAG950.040.2631571
CACGACC7650.039.1176527
TGATTCG3650.038.83561715
CGGTCTA1400.038.57142631
GACGGGT4500.038.54
TAGGGTC8750.038.3142855
AACACGT7050.037.97872541
GCGATCG1550.037.741949
TGACGGG8750.037.542863
ACGGGTA2400.037.5000045
ATAGGGC10050.037.3880584
GACGGGA9100.037.3351634
TAGGGCG8800.037.3295445
CGACGGT1450.037.24138328
ACGGTCT1450.037.24138330
AGACACG8050.037.17391624
GACACGA8150.036.99386625