##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546659_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2004162
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.429836510222227	31.0	31.0	33.0	30.0	34.0
2	31.851899696731103	31.0	31.0	34.0	30.0	34.0
3	31.97760759858734	33.0	31.0	34.0	30.0	34.0
4	35.72632551659996	37.0	35.0	37.0	33.0	37.0
5	35.65426697043453	37.0	35.0	37.0	33.0	37.0
6	35.71632283218622	37.0	35.0	37.0	33.0	37.0
7	36.03713472264218	37.0	35.0	37.0	35.0	37.0
8	36.01897650988293	37.0	35.0	37.0	35.0	37.0
9	37.75763735666079	39.0	38.0	39.0	35.0	39.0
10	37.175330137982854	39.0	37.0	39.0	33.0	39.0
11	37.00104981533429	39.0	37.0	39.0	33.0	39.0
12	36.56581453994238	39.0	35.0	39.0	33.0	39.0
13	36.43117322851147	39.0	35.0	39.0	32.0	39.0
14	37.522695770102416	40.0	35.0	41.0	33.0	41.0
15	37.68366229875629	40.0	35.0	41.0	33.0	41.0
16	37.71780624520373	40.0	35.0	41.0	33.0	41.0
17	37.641543947046195	39.0	35.0	41.0	33.0	41.0
18	37.5533529724643	39.0	36.0	41.0	33.0	41.0
19	37.5037661626156	39.0	36.0	41.0	33.0	41.0
20	37.382862263629384	39.0	35.0	41.0	32.0	41.0
21	37.28934936397357	39.0	35.0	41.0	32.0	41.0
22	37.183933234938095	39.0	35.0	41.0	32.0	41.0
23	37.09607456882228	39.0	35.0	41.0	32.0	41.0
24	36.98528013204521	39.0	35.0	41.0	32.0	41.0
25	36.89050236457931	38.0	35.0	41.0	32.0	41.0
26	36.75980584403855	38.0	35.0	41.0	31.0	41.0
27	36.67993904684352	38.0	35.0	41.0	31.0	41.0
28	36.59849902353203	38.0	35.0	40.0	31.0	41.0
29	36.64675759743973	38.0	35.0	41.0	31.0	41.0
30	36.63125685448581	38.0	35.0	41.0	31.0	41.0
31	36.57046536158255	38.0	35.0	41.0	31.0	41.0
32	36.48943298994792	38.0	35.0	41.0	31.0	41.0
33	36.37217300797041	38.0	35.0	41.0	30.0	41.0
34	36.245943192217	38.0	35.0	41.0	30.0	41.0
35	36.137460444814344	38.0	35.0	41.0	30.0	41.0
36	36.019819256127995	38.0	35.0	41.0	30.0	41.0
37	35.96323151521683	38.0	35.0	41.0	30.0	41.0
38	35.88865421058777	38.0	35.0	41.0	29.0	41.0
39	35.80848953328124	38.0	35.0	41.0	29.0	41.0
40	35.67943359868114	38.0	35.0	40.0	29.0	41.0
41	35.63653686678023	38.0	35.0	40.0	29.0	41.0
42	35.55933402589212	38.0	35.0	40.0	28.0	41.0
43	35.44236094686956	38.0	35.0	40.0	28.0	41.0
44	35.31133710747933	38.0	34.0	40.0	27.0	41.0
45	35.193441947307655	37.0	34.0	40.0	27.0	41.0
46	35.15259045925429	37.0	34.0	40.0	27.0	41.0
47	35.11588933429533	37.0	34.0	40.0	27.0	41.0
48	35.026393574970484	37.0	34.0	40.0	27.0	41.0
49	34.94561567378286	37.0	34.0	40.0	27.0	41.0
50	34.859838176754174	37.0	34.0	40.0	26.0	41.0
51	34.487124294343474	36.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	1.0
11	5.0
12	8.0
13	9.0
14	20.0
15	45.0
16	111.0
17	240.0
18	617.0
19	1280.0
20	2289.0
21	3591.0
22	5741.0
23	8557.0
24	12798.0
25	18905.0
26	25645.0
27	29923.0
28	32468.0
29	36618.0
30	43381.0
31	53486.0
32	68056.0
33	87906.0
34	147646.0
35	233768.0
36	151598.0
37	194572.0
38	292985.0
39	551572.0
40	316.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.16170948256678	23.449701171861356	28.299708307013105	14.088881038558759
2	32.06307673730966	25.14113130575273	29.074645662376597	13.721146294561018
3	29.489332698654103	25.319260618652585	31.54650172990008	13.644904952793238
4	25.140332967095475	28.235242460439824	31.32900434196437	15.295420230500328
5	22.626763704730458	32.36105664112981	29.99263532588683	15.019544328252907
6	20.69787771647202	41.239131367623976	27.25208840403121	10.810902511872793
7	88.11213863949122	3.778087799289678	6.54767428980292	1.562099271416183
8	88.48456362310033	3.1070841578674777	6.257727668721391	2.1506245503108032
9	82.64726104975546	5.119646016639373	8.981409686442513	3.2516832471626547
10	40.26520810193986	27.27878285288315	18.854713341536264	13.601295703640723
11	34.89024340347736	25.05740553907319	23.892779126637468	16.159571930811982
12	31.21783568394172	23.821577297643604	27.024212613551203	17.93637440486348
13	22.748660038459963	31.851467096971202	28.26837351471588	17.131499349852955
14	19.32209072919255	34.320079913699594	29.15163544663555	17.206193910472305
15	19.23522150405007	24.70354192924524	39.24078991618442	16.820446650520267
16	20.556921047300566	22.502222874198793	37.88561004549532	19.055246033005314
17	20.81927508854075	23.04065240235071	29.098795406758537	27.04127710235001
18	23.052128520548738	23.86603478161945	32.68533182447327	20.39650487335854
19	27.19760179067361	25.914172606805234	27.20738143922497	19.68084416329618
20	28.562760894578382	24.944839788400337	26.173882151243262	20.318517165778015
21	24.567624772847704	26.84094399554527	28.516806525620186	20.07462470598684
22	24.041419805384994	24.321736466413395	27.580006007498397	24.056837720703218
23	21.528698777843307	28.08146247658622	26.94143487402715	23.44840387154332
24	21.1916002798177	25.97584426807813	33.615096983178006	19.217458468926164
25	20.833595288205245	26.51512203105338	31.600389589264736	21.050893091476638
26	19.99708606390102	30.645726243686887	28.168680974891252	21.188506717520838
27	20.319465192933507	29.515378497347022	29.2207915328202	20.94436477689927
28	19.060634818941782	28.15685558353067	33.54484318133964	19.237666416187913
29	19.880827996938372	25.468549947559126	32.89160257504134	21.759019480461163
30	21.055234057925457	28.169279728884195	31.50019808777933	19.27528812541102
31	25.868517614843512	26.96817921904517	26.822931479590974	20.34037168652035
32	25.810837646856893	26.453150992784018	28.223067795916695	19.512943564442395
33	24.742460938786383	27.07465763745645	27.39963136712501	20.78325005663215
34	20.792780224353123	28.38258583886931	29.090113473860896	21.73452046291667
35	20.7722729000949	27.574517429229772	30.42059474234119	21.232614928334137
36	25.379984252769987	28.145678842329115	26.95884863598851	19.515488268912392
37	21.687218897474356	29.7273873070141	28.98298640529059	19.60240739022095
38	21.81769737176935	30.86062903098652	26.695995633087545	20.62567796415659
39	22.25887927223448	29.391785693970846	27.15823371563776	21.191101318156917
40	23.20865279353665	27.431864290411657	27.53734478550137	21.822138130550325
41	19.966549610261048	26.32561639228765	28.650178977547725	25.057655019903578
42	22.99839034968231	27.05983847613117	26.56866061725549	23.373110556931024
43	22.601965310189495	26.565417366460398	27.852538866618566	22.980078456731544
44	21.568116749045238	29.110071940292254	28.383783346855196	20.93802796380732
45	20.29351918657274	31.00882064423934	26.99163041710201	21.70602975208591
46	22.553865406089926	29.572010645845992	27.502217884582187	20.371906063481894
47	21.88455823431439	28.50503103042568	28.267525279892542	21.34288545536738
48	23.453393488151157	26.17837280619032	29.154479528102016	21.213754177556503
49	22.169615031120237	26.354107103118412	29.621906811924386	21.854371053836967
50	21.090360958844645	29.833117282934214	28.369014081695994	20.70750767652515
51	20.514160032971386	30.601418448209277	26.98853685480515	21.895884664014186
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1979.0
1	2398.5
2	2818.0
3	10721.5
4	18625.0
5	13208.0
6	7791.0
7	7909.5
8	8028.0
9	8670.0
10	9312.0
11	9413.5
12	9515.0
13	9700.0
14	9885.0
15	9484.0
16	9083.0
17	8862.5
18	8642.0
19	8784.5
20	8927.0
21	8877.0
22	8827.0
23	10461.5
24	12096.0
25	13496.5
26	16757.5
27	18618.0
28	22457.0
29	26296.0
30	31472.0
31	36648.0
32	41579.0
33	46510.0
34	52862.5
35	59215.0
36	63591.5
37	67968.0
38	79123.5
39	90279.0
40	106343.0
41	122407.0
42	139214.0
43	156021.0
44	167970.0
45	179919.0
46	178997.5
47	178076.0
48	166942.5
49	155809.0
50	147853.0
51	139897.0
52	129739.0
53	119581.0
54	109938.0
55	100295.0
56	91813.0
57	83331.0
58	76000.0
59	68669.0
60	63074.0
61	57479.0
62	52351.0
63	47223.0
64	41496.5
65	35770.0
66	32474.5
67	29179.0
68	24219.0
69	19259.0
70	15940.0
71	12621.0
72	10921.0
73	9221.0
74	7539.5
75	4854.5
76	3851.0
77	2790.5
78	1730.0
79	1510.0
80	1290.0
81	863.5
82	437.0
83	292.5
84	148.0
85	109.0
86	70.0
87	55.5
88	41.0
89	27.0
90	13.0
91	10.0
92	7.0
93	4.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2004162.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.425245256518313
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.6777321635179	16.642644625735116
2	6.121715286619063	2.623185028127814
3	2.3147413183666488	1.4878170135420594
4	1.3874096064257777	1.1890236435568733
5	0.9838640703972137	1.0539764503668352
6	0.7342838276058548	0.943932665661027
7	0.6280148427219727	0.941876042103643
8	0.526683369541504	0.9027456291964951
9	0.45725522061467155	0.8817124721843452
>10	7.015531934114737	36.918770953715686
>50	1.8607296239919726	27.394242704904993
>100	0.28991910051529757	8.050338643096797
>500	0.0014130903782709643	0.19326057391538784
>1k	4.7103012609032145E-4	0.231761309658845
>5k	0.0	0.0
>10k+	2.3551506304516072E-4	0.5447122442341024
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10795	0.5386291128162295	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3275	0.16340994390673008	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.989616607839087E-5	0.0	0.0	0.028341022332526014	0.0
2	4.989616607839087E-5	0.0	0.0	0.09475281938286426	0.0
3	4.989616607839087E-5	0.0	0.0	0.16051596627418344	0.0
4	4.989616607839087E-5	0.0	0.0	0.2752771482544824	0.0
5	4.989616607839087E-5	0.0	0.0	0.45585137329217895	0.0
6	4.989616607839087E-5	0.0	0.0	0.9242765804361125	0.0
7	4.989616607839087E-5	0.0	0.0	1.170214783036501	0.0
8	4.989616607839087E-5	0.0	0.0	1.8394720586459579	0.0
9	4.989616607839087E-5	0.0	0.0	2.1916391988272403	0.0
10	4.989616607839087E-5	0.0	0.0	2.688155947473308	0.0
11	4.989616607839087E-5	0.0	0.0	2.984040212318166	0.0
12	4.989616607839087E-5	0.0	0.0	3.2545772247952014	0.0
13	4.989616607839087E-5	0.0	0.0	3.4097044051329184	0.0
14	4.989616607839087E-5	0.0	0.0	3.4836505232610935	0.0
15	4.989616607839087E-5	0.0	0.0	3.5444739497106523	0.0
16	4.989616607839087E-5	0.0	0.0	3.645463789853315	0.0
17	4.989616607839087E-5	0.0	0.0	3.75787985202793	0.0
18	4.989616607839087E-5	0.0	0.0	3.93461207227759	0.0
19	4.989616607839087E-5	0.0	0.0	4.0134480146814475	0.0
20	4.989616607839087E-5	0.0	0.0	4.099019939505888	0.0
21	4.989616607839087E-5	0.0	0.0	4.199560714153846	0.0
22	4.989616607839087E-5	0.0	0.0	4.314521480798458	0.0
23	4.989616607839087E-5	0.0	0.0	4.444351304934432	0.0
24	4.989616607839087E-5	0.0	0.0	4.546887926225525	0.0
25	4.989616607839087E-5	0.0	0.0	4.645083581067798	0.0
26	4.989616607839087E-5	0.0	0.0	4.734896680008902	0.0
27	4.989616607839087E-5	0.0	0.0	4.829449914727452	0.0
28	4.989616607839087E-5	0.0	0.0	4.926298373085609	0.0
29	4.989616607839087E-5	0.0	0.0	5.032028349005719	0.0
30	9.979233215678174E-5	0.0	0.0	5.200178428689896	0.0
31	9.979233215678174E-5	0.0	0.0	5.315837741659607	0.0
32	9.979233215678174E-5	0.0	0.0	5.450058428410478	0.0
33	9.979233215678174E-5	0.0	0.0	5.567913172687637	0.0
34	9.979233215678174E-5	0.0	0.0	5.681027781187349	0.0018461581449004622
35	9.979233215678174E-5	0.0	0.0	5.811955320977047	0.0018461581449004622
36	9.979233215678174E-5	0.0	0.0	5.931157261738322	0.0018461581449004622
37	9.979233215678174E-5	0.0	0.0	6.059839474054493	0.0018461581449004622
38	9.979233215678174E-5	0.0	0.0	6.192413587324777	0.0018461581449004622
39	9.979233215678174E-5	0.0	0.0	6.332422229340742	0.0018461581449004622
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTAAG	20	7.034545E-4	45.0	1
CGTTTTT	7075	0.0	40.92933	1
GTTTACG	50	1.0822987E-9	40.5	1
TCGATAG	95	0.0	40.263157	1
CACGACC	765	0.0	39.11765	27
TGATTCG	365	0.0	38.835617	15
CGGTCTA	140	0.0	38.571426	31
GACGGGT	450	0.0	38.5	4
TAGGGTC	875	0.0	38.314285	5
AACACGT	705	0.0	37.978725	41
GCGATCG	155	0.0	37.74194	9
TGACGGG	875	0.0	37.54286	3
ACGGGTA	240	0.0	37.500004	5
ATAGGGC	1005	0.0	37.388058	4
GACGGGA	910	0.0	37.335163	4
TAGGGCG	880	0.0	37.329544	5
CGACGGT	145	0.0	37.241383	28
ACGGTCT	145	0.0	37.241383	30
AGACACG	805	0.0	37.173916	24
GACACGA	815	0.0	36.993866	25
>>END_MODULE