##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546658_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1781474
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.36928184189048	31.0	31.0	33.0	30.0	34.0
2	31.781576941341832	31.0	31.0	34.0	30.0	34.0
3	31.881407755600137	31.0	31.0	34.0	30.0	34.0
4	35.63130475100956	37.0	35.0	37.0	33.0	37.0
5	35.54563243695951	37.0	35.0	37.0	33.0	37.0
6	35.61465617797397	37.0	35.0	37.0	33.0	37.0
7	35.97940357254723	37.0	35.0	37.0	35.0	37.0
8	35.95587979392346	37.0	35.0	37.0	35.0	37.0
9	37.67277658837569	39.0	37.0	39.0	35.0	39.0
10	37.13758494370392	39.0	37.0	39.0	33.0	39.0
11	36.868772151600304	39.0	37.0	39.0	33.0	39.0
12	36.289577619431995	38.0	35.0	39.0	32.0	39.0
13	36.07877128714761	38.0	35.0	39.0	32.0	39.0
14	37.1115042936355	39.0	35.0	41.0	32.0	41.0
15	37.323040920047106	39.0	35.0	41.0	32.0	41.0
16	37.40721110720673	39.0	35.0	41.0	32.0	41.0
17	37.331272305966856	39.0	35.0	41.0	32.0	41.0
18	37.221637250950614	39.0	35.0	41.0	32.0	41.0
19	37.15628743388901	39.0	35.0	41.0	32.0	41.0
20	37.02377244910675	38.0	35.0	41.0	32.0	41.0
21	36.90875757939774	38.0	35.0	41.0	32.0	41.0
22	36.80096594168649	38.0	35.0	41.0	31.0	41.0
23	36.721185939283984	38.0	35.0	41.0	31.0	41.0
24	36.61829024728961	38.0	35.0	41.0	31.0	41.0
25	36.4974807378609	38.0	35.0	40.0	31.0	41.0
26	36.35970830896213	38.0	35.0	40.0	31.0	41.0
27	36.2537696312155	38.0	35.0	40.0	31.0	41.0
28	36.16441721855048	38.0	35.0	40.0	30.0	41.0
29	36.20372736284672	38.0	35.0	40.0	30.0	41.0
30	36.17714095181855	38.0	35.0	40.0	30.0	41.0
31	36.072847540856614	38.0	35.0	40.0	30.0	41.0
32	35.91826262971001	38.0	35.0	40.0	30.0	41.0
33	35.790555461376364	38.0	35.0	40.0	30.0	41.0
34	35.62640094663183	38.0	35.0	40.0	29.0	41.0
35	35.517028034088625	38.0	35.0	40.0	29.0	41.0
36	35.36831803326908	38.0	34.0	40.0	27.0	41.0
37	35.31691733923706	38.0	34.0	40.0	27.0	41.0
38	35.264354686063335	38.0	34.0	40.0	27.0	41.0
39	35.19932426743247	37.0	34.0	40.0	27.0	41.0
40	35.068054319063876	37.0	34.0	40.0	26.0	41.0
41	35.031187656962715	37.0	34.0	40.0	26.0	41.0
42	34.95598813117677	37.0	34.0	40.0	26.0	41.0
43	34.825409744964	37.0	34.0	40.0	25.0	41.0
44	34.6791842036426	37.0	34.0	40.0	24.0	41.0
45	34.564148003282675	36.0	34.0	40.0	24.0	41.0
46	34.53663763827033	36.0	34.0	40.0	24.0	41.0
47	34.50143701227186	36.0	34.0	40.0	24.0	41.0
48	34.410077273089584	36.0	34.0	40.0	24.0	41.0
49	34.335002363211586	36.0	34.0	40.0	24.0	41.0
50	34.21595768447926	36.0	33.0	40.0	24.0	41.0
51	33.83929319204209	35.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	4.0
12	6.0
13	7.0
14	20.0
15	54.0
16	130.0
17	372.0
18	867.0
19	1662.0
20	2908.0
21	4408.0
22	6943.0
23	9902.0
24	14476.0
25	20248.0
26	26560.0
27	31140.0
28	33742.0
29	38201.0
30	44460.0
31	53429.0
32	65706.0
33	85537.0
34	144210.0
35	231462.0
36	131004.0
37	162076.0
38	238172.0
39	433494.0
40	271.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.10124088255007	23.92137073008082	28.367351979316002	14.610036408053107
2	32.79705457390902	25.18801846111703	28.13226575296636	13.882661212007585
3	29.613118125776744	25.11437158218419	31.615841713098252	13.656668578940808
4	25.56719884769578	28.412819945730334	30.710467848534417	15.309513358039467
5	23.281900269103	32.58762126194376	29.58830721077041	14.542171258182831
6	21.40805871991396	40.77460574782455	26.75402503769351	11.06331049456798
7	86.4234897618489	4.2366040705617936	7.49048260036352	1.8494235672257917
8	86.3416474223031	3.700924066250756	7.177539498190824	2.779889013255316
9	80.5962365995799	5.64319209822877	9.931101997559324	3.829469304632007
10	45.69536237969232	26.155756412947927	15.633514718710462	12.515366488649288
11	37.254037948350636	24.165494416421456	24.507346163906966	14.07312147132094
12	33.117856336943454	22.958628641226312	27.182546587825584	16.74096843400465
13	22.59219051190194	32.80586750073254	28.79278619839526	15.809155788970259
14	18.176521240276312	35.68326004196525	30.06330712657047	16.07691159118797
15	16.968139866200683	24.055024098022198	42.89380591577536	16.08303012000175
16	19.319170529572702	21.58560832209732	40.67962821798129	18.41559293034869
17	19.892179172976984	21.75687099559129	30.17102691366812	28.179922917763605
18	22.57894305502073	22.906705346246984	34.40757485093805	20.106776747794243
19	28.168527859514086	25.061325621367477	27.27090038922825	19.499246129890192
20	28.98341485758423	25.13682489893201	26.208970773640257	19.670789469843513
21	24.127323777950167	27.666976896659733	29.065032663962537	19.14066666142756
22	23.747357525285242	24.782848360402678	27.2062909702864	24.263503144025677
23	20.650034746507668	29.424734798262563	26.669712833305454	23.255517621924316
24	20.93294653753016	24.752480249501254	35.16840548893781	19.146167724030775
25	19.97222524718295	25.65751731431388	33.04376039167566	21.326497046827512
26	18.99623570144723	31.06640905227918	28.510155073832117	21.42720017244147
27	19.857881731644696	30.76177367730318	28.969718334368054	20.410626256684072
28	18.67105554164697	28.298869363235163	34.70648463014335	18.323590464974508
29	19.430202180890653	25.470874118847654	33.73582774713524	21.363095953126457
30	21.87710850677585	28.173804388949826	30.6034216609392	19.345665443335125
31	27.14257968401447	26.30265723777052	26.719783729653084	19.83497934856192
32	28.454134048546315	25.977420944678396	27.307836095278404	18.260608911496885
33	27.094305053006668	27.144825015689257	25.94149563788189	19.819374293422186
34	21.654989070848075	27.427905206587354	29.310391282724307	21.606714439840268
35	21.32969664446408	26.963682882826244	30.70844705002711	20.998173422682566
36	26.979456337841583	26.35194226803198	27.735571779324314	18.933029614802123
37	21.408114853205827	30.619082849370805	29.644833435683037	18.327968861740334
38	21.440335362738946	30.901601707350206	26.49480149583996	21.163261434070886
39	20.79149064201891	30.22643047274336	28.024153032825627	20.957925852412103
40	23.488077850139828	26.116070175596164	27.97234200443004	22.42350996983397
41	19.046418864378598	25.66593730809431	28.97825059473223	26.309393232794864
42	23.016782731603154	25.777530292330958	26.565810110054933	24.639876866010955
43	22.1156749972214	25.941214971422543	28.43993232570332	23.503177705652732
44	20.92166374586438	28.91695304001069	29.033878686974944	21.12750452714999
45	19.19921368484749	32.409510326841705	26.725004125796954	21.66627186251385
46	22.39987785395689	29.18493337539588	27.73310191448205	20.682086856165174
47	21.769725519429418	27.318725953901097	28.30549309167577	22.606055434993717
48	22.616103294238364	25.28602718872125	30.315401740356577	21.782467776683802
49	22.05224437740882	24.93452051503418	30.527080383996623	22.48615472356038
50	20.613884906543685	30.13577520637405	28.149779340029664	21.10056054705261
51	20.17307016549217	30.70260918767268	26.549363055537157	22.57495759129799
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2185.0
1	2671.5
2	3158.0
3	10381.5
4	17605.0
5	12635.5
6	7666.0
7	8072.5
8	8479.0
9	9034.0
10	9589.0
11	9878.0
12	10167.0
13	10162.5
14	10158.0
15	9999.0
16	9840.0
17	9543.0
18	9246.0
19	9201.0
20	9156.0
21	9453.0
22	9750.0
23	10446.0
24	11142.0
25	11884.5
26	15002.5
27	17378.0
28	19131.5
29	20885.0
30	24570.0
31	28255.0
32	31638.0
33	35021.0
34	42255.0
35	49489.0
36	53336.5
37	57184.0
38	65361.5
39	73539.0
40	89407.0
41	105275.0
42	126575.5
43	147876.0
44	159680.5
45	171485.0
46	169806.5
47	168128.0
48	155421.0
49	142714.0
50	131575.5
51	120437.0
52	108297.0
53	96157.0
54	88630.0
55	81103.0
56	74700.5
57	68298.0
58	63818.5
59	59339.0
60	55499.0
61	51659.0
62	46853.5
63	42048.0
64	38176.5
65	34305.0
66	29368.5
67	24432.0
68	21169.5
69	17907.0
70	15589.5
71	13272.0
72	11517.5
73	9763.0
74	8233.0
75	5113.5
76	3524.0
77	2801.0
78	2078.0
79	1635.5
80	1193.0
81	874.5
82	556.0
83	443.5
84	331.0
85	248.5
86	166.0
87	125.5
88	85.0
89	68.0
90	51.0
91	49.0
92	47.0
93	30.5
94	14.0
95	10.5
96	7.0
97	4.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1781474.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.509871393994736
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.34190394208512	18.08485925285015
2	6.257567545575059	2.817140813802598
3	2.184759917722741	1.4753599432408027
4	1.2357438421777156	1.1126573985336523
5	0.8240780296039236	0.9274945232500456
6	0.605042392881854	0.8171655871011221
7	0.48429209199415574	0.7630946895541979
8	0.40559034957387724	0.7303829286042677
9	0.3561712556084983	0.7215632242186432
>10	5.012514676966146	27.931646769552103
>50	1.7460142683366944	27.585483130448818
>100	0.5435586812493807	15.840818681629242
>500	0.0017582766885532388	0.2795944123636716
>1k	7.535471522371023E-4	0.3372660606166433
>5k	0.0	0.0
>10k+	2.511823840790341E-4	0.5754725842340647
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10178	0.5713246446481958	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2867	0.16093414778997617	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1226658373908348E-4	0.0	0.0	0.04485050020376385	0.0
2	1.1226658373908348E-4	0.0	0.0	0.15498401885180474	0.0
3	1.1226658373908348E-4	0.0	0.0	0.24642515130728823	0.0
4	1.1226658373908348E-4	0.0	0.0	0.41841755759556415	0.0
5	1.1226658373908348E-4	0.0	0.0	0.6769674999466734	0.0
6	1.1226658373908348E-4	0.0	0.0	1.3464131387828282	0.0
7	1.1226658373908348E-4	0.0	0.0	1.6674394349847375	0.0
8	1.1226658373908348E-4	0.0	0.0	2.5691646355770557	0.0
9	1.1226658373908348E-4	0.0	0.0	3.0766657273695825	0.0
10	1.1226658373908348E-4	0.0	0.0	3.7269699136782237	0.0
11	1.1226658373908348E-4	0.0	0.0	4.172275318079298	0.0
12	1.1226658373908348E-4	0.0	0.0	4.5654328943335685	0.0
13	1.1226658373908348E-4	0.0	0.0	4.774136473504525	0.0
14	1.1226658373908348E-4	0.0	0.0	4.873548533405483	0.0
15	1.1226658373908348E-4	0.0	0.0	4.957916871085405	0.0
16	1.1226658373908348E-4	0.0	0.0	5.101730364855171	0.0
17	1.1226658373908348E-4	0.0	0.0	5.242905593907068	0.0
18	1.1226658373908348E-4	0.0	0.0	5.4716487582754505	0.0
19	1.1226658373908348E-4	0.0	0.0	5.577179346990189	0.0
20	1.1226658373908348E-4	0.0	0.0	5.690287930107315	0.0
21	1.1226658373908348E-4	0.0	0.0	5.819450634699131	0.0
22	1.1226658373908348E-4	0.0	0.0	5.9563036002770735	0.0
23	1.1226658373908348E-4	0.0	0.0	6.111848952047574	0.0
24	1.6839987560862522E-4	0.0	0.0	6.242976321854823	0.0
25	1.6839987560862522E-4	0.0	0.0	6.349012110196388	0.0
26	1.6839987560862522E-4	0.0	0.0	6.451848300901388	0.0
27	1.6839987560862522E-4	0.0	0.0	6.559568087999039	0.0
28	1.6839987560862522E-4	0.0	0.0	6.6648741435462995	0.0
29	1.6839987560862522E-4	0.0	0.0	6.781968190386164	0.0
30	1.6839987560862522E-4	0.0	0.0	6.965973121134521	0.0
31	1.6839987560862522E-4	0.0	0.0	7.099289689324682	0.0
32	1.6839987560862522E-4	0.0	0.0	7.235132255648974	0.0
33	1.6839987560862522E-4	0.0	0.0	7.364631759992006	0.0
34	1.6839987560862522E-4	0.0	0.0	7.4926156654545615	0.0
35	1.6839987560862522E-4	0.0	0.0	7.644287820086063	0.0
36	1.6839987560862522E-4	0.0	0.0	7.776369455855095	0.0
37	1.6839987560862522E-4	0.0	0.0	7.916365885777733	0.0
38	1.6839987560862522E-4	0.0	0.0	8.05995484638002	0.0
39	1.6839987560862522E-4	0.0	0.0	8.250078305942157	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTCGAC	25	3.8916725E-5	45.0	9
CAATACG	25	3.8916725E-5	45.0	1
CGTTACC	30	2.1661763E-6	44.999996	27
ATACGTC	30	2.1661763E-6	44.999996	15
GTATGCG	115	0.0	43.043476	1
TCGTAAG	105	0.0	40.714287	1
CGTTTTT	6720	0.0	40.680805	1
CGAGGGT	165	0.0	39.545456	4
TGTCACG	40	3.459645E-7	39.375004	1
AGGGTAC	880	0.0	38.863636	6
AGGGCGA	2015	0.0	38.858562	6
CTATCGA	35	6.250226E-6	38.571426	2
TTAATCG	100	0.0	38.25	20
GGTACCT	1175	0.0	38.106384	8
ACGGGAT	630	0.0	37.857143	5
TACGGGA	345	0.0	37.826088	4
GTCGCAT	30	1.140174E-4	37.499996	9
TATGCGA	30	1.140174E-4	37.499996	37
ACTTACG	30	1.140174E-4	37.499996	1
TACGTAC	30	1.140174E-4	37.499996	11
>>END_MODULE