FastQCFastQC Report
Thu 26 May 2016
SRR1546657_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546657_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2326131
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203830.8762619130220954No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC39110.16813326506546708No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT139800.041.990341
CATACGA2200.040.90909218
AGGGCGA25600.039.199226
CGTTAGG3350.038.2835852
TTACGGG6350.038.2677153
GCGTAAG1650.038.181821
CGTAAGG3850.037.9870152
CCTCGAA301.1402811E-437.50000440
TACGCTA301.1402811E-437.5000041
CGGTCTA900.037.531
GGGCGAT49550.037.416757
CATATGC13450.037.30483233
ACGGGTA2600.037.2115365
TAGGGTA12100.037.0041355
TCGTAAG1100.036.818181
TATGGGC10700.036.7990654
ACACGAC8100.036.66666826
GGCGATT12900.036.453498
TACGGGA5450.036.3302764
TAGGGCG10800.036.255
Produced by FastQC (version 0.11.5)