##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546657_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2326131
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.400841999010375	31.0	31.0	33.0	30.0	34.0
2	31.81394384065214	31.0	31.0	34.0	30.0	34.0
3	31.886656426486727	31.0	31.0	34.0	30.0	34.0
4	35.69242574902273	37.0	35.0	37.0	33.0	37.0
5	35.633809531793354	37.0	35.0	37.0	33.0	37.0
6	35.69545008428158	37.0	35.0	37.0	33.0	37.0
7	35.98697364851765	37.0	35.0	37.0	35.0	37.0
8	35.95978085499054	37.0	35.0	37.0	35.0	37.0
9	37.721829939930295	39.0	37.0	39.0	35.0	39.0
10	37.19332703102276	39.0	37.0	39.0	33.0	39.0
11	36.98398585462298	39.0	37.0	39.0	33.0	39.0
12	36.48597864866596	39.0	35.0	39.0	32.0	39.0
13	36.33258316062165	39.0	35.0	39.0	32.0	39.0
14	37.39337853285133	40.0	35.0	41.0	32.0	41.0
15	37.56401982519471	40.0	35.0	41.0	33.0	41.0
16	37.605906546105956	40.0	35.0	41.0	33.0	41.0
17	37.53526822006155	39.0	35.0	41.0	33.0	41.0
18	37.41427202509231	39.0	36.0	41.0	33.0	41.0
19	37.305734715714635	39.0	35.0	41.0	32.0	41.0
20	37.130868811773716	39.0	35.0	41.0	32.0	41.0
21	37.03168179264195	39.0	35.0	41.0	32.0	41.0
22	36.935468810655976	38.0	35.0	41.0	32.0	41.0
23	36.860936894783656	38.0	35.0	41.0	32.0	41.0
24	36.723971263871206	38.0	35.0	41.0	31.0	41.0
25	36.61329821923185	38.0	35.0	40.0	31.0	41.0
26	36.49575926721238	38.0	35.0	40.0	31.0	41.0
27	36.38880269425927	38.0	35.0	40.0	31.0	41.0
28	36.29977116508056	38.0	35.0	40.0	31.0	41.0
29	36.28350123015428	38.0	35.0	40.0	31.0	41.0
30	36.24426569268885	38.0	35.0	40.0	31.0	41.0
31	36.146410498806816	38.0	35.0	40.0	30.0	41.0
32	35.96827607731465	38.0	35.0	40.0	30.0	41.0
33	35.7507324394026	38.0	35.0	40.0	29.0	41.0
34	35.528980955930685	38.0	35.0	40.0	27.0	41.0
35	35.35212419248959	38.0	35.0	40.0	27.0	41.0
36	35.19004862580826	38.0	34.0	40.0	26.0	41.0
37	35.11523469658415	38.0	34.0	40.0	25.0	41.0
38	35.024361482650804	38.0	34.0	40.0	25.0	41.0
39	34.91771830563283	37.0	34.0	40.0	25.0	41.0
40	34.76015753197047	37.0	34.0	40.0	24.0	41.0
41	34.69961837918844	37.0	34.0	40.0	23.0	41.0
42	34.620707948090626	37.0	34.0	40.0	23.0	41.0
43	34.495773883758055	37.0	34.0	40.0	23.0	41.0
44	34.328515032042475	37.0	34.0	40.0	23.0	41.0
45	34.22450240334702	36.0	34.0	40.0	23.0	41.0
46	34.21132257813511	36.0	34.0	40.0	23.0	41.0
47	34.17473521482668	36.0	33.0	40.0	23.0	41.0
48	34.06844971327926	36.0	33.0	40.0	23.0	41.0
49	33.99164406475818	36.0	33.0	40.0	23.0	41.0
50	33.87436305177997	35.0	33.0	40.0	23.0	41.0
51	33.51363530256894	35.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	3.0
12	9.0
13	16.0
14	23.0
15	59.0
16	204.0
17	463.0
18	1014.0
19	1990.0
20	3469.0
21	5776.0
22	8942.0
23	13533.0
24	20434.0
25	30480.0
26	40940.0
27	45937.0
28	47003.0
29	49837.0
30	56300.0
31	66853.0
32	82126.0
33	106618.0
34	179059.0
35	280766.0
36	177658.0
37	222761.0
38	320622.0
39	562860.0
40	375.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.98662027203111	23.401261579850836	27.402111059093404	16.21000708902465
2	33.43233893533941	24.784201749600516	28.134228037887805	13.649231277172266
3	28.846010822262375	24.696416495889526	32.79037165146761	13.667201030380491
4	24.83149057383269	28.002421187800685	32.25458927291713	14.911498965449496
5	22.57362117610745	31.5709218440406	31.315304254145616	14.540152725706335
6	20.529325304550774	39.81645917620289	29.129872737176022	10.524342782070313
7	84.69080202275796	4.357063295231438	9.180566356752909	1.7715683252576917
8	85.33573560560433	3.4909469844991534	8.900229608736568	2.273087801159952
9	80.05125248749962	5.260838705988614	11.414232474439316	3.2736763320724416
10	42.7472915325921	23.372630346270267	19.249947659869544	14.630130461268088
11	35.856922933403155	24.83149057383269	24.079684248221618	15.231902244542548
12	31.864714412043	22.695497373105812	27.6045502166473	17.835237998203883
13	22.61557926015345	30.98011247001996	29.17724754108861	17.227060728737978
14	19.16770809554578	33.079263377685955	30.83936373316894	16.913664793599327
15	19.292722550879553	23.558088516940792	40.25383781051024	16.895351121669417
16	20.80050521660216	21.65015641853361	38.92037894684349	18.628959418020738
17	20.900499584933094	21.748689132297365	30.73571522842007	26.615096054349475
18	23.411106253259167	23.25402137712794	33.418324247430604	19.916548122182284
19	27.201477474828373	25.03547736563418	28.019359184843847	19.7436859746936
20	28.501318283450072	24.18363368185197	27.557777270497663	19.757270764200296
21	24.248677310091306	26.723860350083466	29.86908303960525	19.15837930021998
22	23.78619260910069	24.06278924101867	28.663905859128313	23.487112290752325
23	21.463365562816538	27.81756487489312	27.783860840167645	22.935208722122702
24	21.50988056992491	24.922371096038873	34.68721237110034	18.88053596293588
25	20.787049396616098	25.301111588298337	32.601388313899776	21.310450701185786
26	19.133574162418196	30.154879497328395	29.472587743338618	21.23895859691479
27	20.149123157724137	28.881477440436505	30.58245644806763	20.386942953771737
28	19.394135583937448	27.41406223467208	34.151472982390075	19.0403291990004
29	20.021443332297277	25.240109005038843	33.799644130102735	20.938803532561153
30	20.839282052472537	27.712497705417277	31.95452018824391	19.49370005386627
31	25.74532560719925	26.266835358799657	28.49104371163963	19.496795322361464
32	26.524903369586667	26.705030800071018	28.268485308866953	18.50158052147536
33	25.184867060367623	27.337411349575753	27.45099050741338	20.02673108264324
34	21.25314524418444	28.230138371398688	29.33596603114786	21.180750353269012
35	20.872685158316536	28.192479271373795	30.382209772364497	20.552625797945172
36	26.080345431964062	27.32838348313143	27.14546171303336	19.445809371871146
37	22.103011395316948	30.031455666082437	28.08225332107263	19.78327961752799
38	22.163885009055807	30.182607944264532	26.34069190428226	21.312815142397397
39	22.468296067590344	29.186533346574205	26.74797765044187	21.59719293539358
40	24.323823550780244	26.394515184226513	27.31140249624806	21.970258768745182
41	20.280027221166822	25.731697827852347	28.331078516214266	25.657196434766572
42	23.326158329002105	26.316918522645544	26.325645460208392	24.031277688143962
43	22.52474172778747	26.154760845369413	28.020090012127437	23.30040741471568
44	21.54298274688743	28.385460664081258	28.458156483878167	21.61340010515315
45	20.365963911748736	31.259331482190817	26.389012484679498	21.985692121380954
46	22.358972903933612	29.26722527665037	26.931716227503955	21.442085591912065
47	21.814764516701768	28.18766440927016	27.950747399866987	22.046823674161086
48	22.783712525218917	26.223630569387534	29.56213558049826	21.430521324895288
49	22.331201467157268	25.647652690239713	29.497091952258923	22.524053890344096
50	20.857681704082875	29.57537645128327	28.427203798926197	21.139738045707656
51	20.601720195466207	30.26983433005278	26.423834255250455	22.704611219230557
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2610.0
1	3208.0
2	3806.0
3	18800.5
4	33795.0
5	23838.0
6	13881.0
7	14586.5
8	15292.0
9	16107.0
10	16922.0
11	17474.0
12	18026.0
13	17789.5
14	17553.0
15	17109.0
16	16665.0
17	16400.0
18	16135.0
19	15350.5
20	14566.0
21	13617.0
22	12668.0
23	13644.0
24	14620.0
25	16136.0
26	19372.5
27	21093.0
28	22634.5
29	24176.0
30	28318.5
31	32461.0
32	37401.0
33	42341.0
34	47288.0
35	52235.0
36	60319.0
37	68403.0
38	77992.5
39	87582.0
40	109863.0
41	132144.0
42	152292.0
43	172440.0
44	184372.5
45	196305.0
46	194902.0
47	193499.0
48	186541.0
49	179583.0
50	168879.5
51	158176.0
52	146288.0
53	134400.0
54	127503.0
55	120606.0
56	109307.0
57	98008.0
58	92453.0
59	86898.0
60	81584.5
61	76271.0
62	68250.0
63	60229.0
64	56686.0
65	53143.0
66	44680.0
67	36217.0
68	32044.0
69	27871.0
70	23633.0
71	19395.0
72	17203.5
73	15012.0
74	12573.0
75	8361.0
76	6588.0
77	5024.5
78	3461.0
79	2626.5
80	1792.0
81	1303.5
82	815.0
83	557.0
84	299.0
85	260.5
86	222.0
87	158.0
88	94.0
89	62.5
90	31.0
91	18.0
92	5.0
93	4.0
94	3.0
95	3.0
96	3.0
97	3.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2326131.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.063760139602383
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.23327072376071	18.274171510489968
2	6.6274315603967295	3.0570698370124183
3	2.429631028527999	1.6810928180911562
4	1.408050145738318	1.2989972330336301
5	0.9580309284037043	1.1047897769511812
6	0.7509540064580444	1.0391893848493055
7	0.5652929543885528	0.9126446776037342
8	0.47019370808571687	0.8675547921951356
9	0.3908658045838654	0.8113351647325472
>10	5.079529275965965	27.81907105967384
>50	1.5331450632776042	24.75058303955507
>100	0.5509568869919993	16.882553946584505
>500	0.001702230056173085	0.26984586876837235
>1k	7.565466916324823E-4	0.3419530820037619
>5k	0.0	0.0
>10k+	1.8913667290812057E-4	0.8891478084555019
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20383	0.8762619130220954	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3911	0.16813326506546708	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.597968042212584E-5	0.0	0.0	0.04273190116979654	0.0
2	8.597968042212584E-5	0.0	0.0	0.12118835955498637	0.0
3	8.597968042212584E-5	0.0	0.0	0.21314362776644996	0.0
4	8.597968042212584E-5	0.0	0.0	0.3403075751107741	0.0
5	8.597968042212584E-5	0.0	0.0	0.5325151506944363	0.0
6	8.597968042212584E-5	0.0	0.0	1.0541108819752627	0.0
7	8.597968042212584E-5	0.0	0.0	1.3198310843198426	0.0
8	8.597968042212584E-5	0.0	0.0	2.005905944248196	0.0
9	8.597968042212584E-5	0.0	0.0	2.384431487306605	0.0
10	8.597968042212584E-5	0.0	0.0	2.893603154766434	0.0
11	8.597968042212584E-5	0.0	0.0	3.2449161289712403	0.0
12	8.597968042212584E-5	0.0	0.0	3.5619662005278294	0.0
13	8.597968042212584E-5	0.0	0.0	3.7527121215443153	0.0
14	8.597968042212584E-5	0.0	0.0	3.843162745348392	0.0
15	8.597968042212584E-5	0.0	0.0	3.9152137175421333	0.0
16	8.597968042212584E-5	0.0	0.0	4.0384655894272505	0.0
17	8.597968042212584E-5	0.0	0.0	4.174915342257164	0.0
18	8.597968042212584E-5	0.0	0.0	4.375720885883039	0.0
19	8.597968042212584E-5	0.0	0.0	4.466902336970704	0.0
20	8.597968042212584E-5	0.0	0.0	4.5672406240233245	0.0
21	8.597968042212584E-5	0.0	0.0	4.6861935118873355	0.0
22	8.597968042212584E-5	0.0	0.0	4.810692089138574	0.0
23	8.597968042212584E-5	0.0	0.0	4.9551809420879565	0.0
24	8.597968042212584E-5	0.0	0.0	5.071769388740359	0.0
25	8.597968042212584E-5	0.0	0.0	5.178814090865906	0.0
26	8.597968042212584E-5	0.0	0.0	5.2855578641099745	0.0
27	8.597968042212584E-5	0.0	0.0	5.403822914530609	0.0
28	8.597968042212584E-5	0.0	0.0	5.511641433779955	0.0
29	8.597968042212584E-5	0.0	0.0	5.636354960232248	0.0
30	8.597968042212584E-5	0.0	0.0	5.810248863885998	0.0
31	8.597968042212584E-5	0.0	0.0	5.939003435318131	0.0
32	8.597968042212584E-5	0.0	0.0	6.077000822395643	0.0
33	8.597968042212584E-5	0.0	0.0	6.209151591204451	0.0
34	8.597968042212584E-5	0.0	0.0	6.3437957707454995	0.0
35	8.597968042212584E-5	0.0	0.0	6.494647120046119	0.0
36	8.597968042212584E-5	0.0	0.0	6.633547293768064	0.0
37	8.597968042212584E-5	0.0	0.0	6.779755740325888	0.0
38	8.597968042212584E-5	0.0	0.0	6.9475020968294565	0.0
39	8.597968042212584E-5	0.0	0.0	7.157808395141976	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	13980	0.0	41.99034	1
CATACGA	220	0.0	40.909092	18
AGGGCGA	2560	0.0	39.19922	6
CGTTAGG	335	0.0	38.283585	2
TTACGGG	635	0.0	38.267715	3
GCGTAAG	165	0.0	38.18182	1
CGTAAGG	385	0.0	37.987015	2
CCTCGAA	30	1.1402811E-4	37.500004	40
TACGCTA	30	1.1402811E-4	37.500004	1
CGGTCTA	90	0.0	37.5	31
GGGCGAT	4955	0.0	37.41675	7
CATATGC	1345	0.0	37.304832	33
ACGGGTA	260	0.0	37.211536	5
TAGGGTA	1210	0.0	37.004135	5
TCGTAAG	110	0.0	36.81818	1
TATGGGC	1070	0.0	36.799065	4
ACACGAC	810	0.0	36.666668	26
GGCGATT	1290	0.0	36.45349	8
TACGGGA	545	0.0	36.330276	4
TAGGGCG	1080	0.0	36.25	5
>>END_MODULE