FastQCFastQC Report
Thu 26 May 2016
SRR1546656_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546656_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1647585
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57290.34772105839759404No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA22690.13771671871253985No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGC254.4436827E-544.023
CCGTATC254.4436827E-544.023
TACGATA207.857478E-444.018
TATTCGA254.4436827E-544.010
AATGTCG254.4436827E-544.019
GTTGCGT254.4436827E-544.011
TATCGCG351.4465877E-743.9999961
CGTAAGG3300.041.3333322
ACGCTAG557.8216544E-1140.01
CGTTTTT47500.039.9242131
ACTAACG501.3478711E-939.6000021
CGGTCTA1450.039.44827731
ATGTACG1900.039.368421
CGGGTAT1800.039.111116
CCACGAA452.3519533E-839.1111114
GCGATCG404.1259955E-738.59
ATGGGCG6900.038.260875
ACGTAGG2300.038.260872
AGGGCGA16800.038.1071436
AGGGATC13400.038.089556
Produced by FastQC (version 0.11.5)