##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546656_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1647585
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.892515408916687	31.0	31.0	33.0	28.0	34.0
2	31.262799187902292	31.0	31.0	34.0	30.0	34.0
3	31.419589277639698	31.0	31.0	34.0	30.0	34.0
4	35.20110525405366	37.0	35.0	37.0	33.0	37.0
5	34.974962748507664	35.0	35.0	37.0	32.0	37.0
6	35.04733473538543	37.0	35.0	37.0	32.0	37.0
7	35.58042953777802	36.0	35.0	37.0	35.0	37.0
8	35.60675473496056	37.0	35.0	37.0	35.0	37.0
9	37.36634468024411	39.0	37.0	39.0	35.0	39.0
10	36.6108589238188	39.0	35.0	39.0	32.0	39.0
11	36.21105678917932	38.0	35.0	39.0	31.0	39.0
12	35.43423616990929	37.0	35.0	39.0	30.0	39.0
13	35.131037245422846	37.0	34.0	39.0	30.0	39.0
14	35.98494705887708	38.0	34.0	40.0	29.0	41.0
15	36.278610208274536	38.0	35.0	40.0	30.0	41.0
16	36.39966010858317	38.0	35.0	40.0	31.0	41.0
17	36.312174485686626	38.0	35.0	40.0	31.0	41.0
18	36.24977345630119	38.0	35.0	40.0	30.0	41.0
19	36.18877994155081	37.0	35.0	40.0	30.0	41.0
20	36.012492830415425	37.0	34.0	40.0	30.0	41.0
21	35.85511157239232	37.0	34.0	40.0	30.0	41.0
22	35.80602761010813	37.0	34.0	40.0	30.0	41.0
23	35.616034984537976	37.0	34.0	40.0	29.0	41.0
24	35.579818340176686	37.0	34.0	40.0	29.0	41.0
25	35.41200545040165	36.0	34.0	40.0	29.0	41.0
26	35.28234294437009	36.0	34.0	40.0	29.0	41.0
27	35.09010946324469	36.0	34.0	40.0	27.0	41.0
28	35.05630968963665	36.0	34.0	40.0	27.0	41.0
29	34.95653881286853	36.0	34.0	40.0	27.0	41.0
30	34.90856556717863	36.0	33.0	40.0	27.0	41.0
31	34.801843303987354	36.0	33.0	40.0	26.0	41.0
32	34.57876346288659	36.0	33.0	40.0	25.0	41.0
33	34.35417353277676	36.0	33.0	40.0	24.0	41.0
34	34.247083458516556	36.0	33.0	40.0	24.0	41.0
35	34.03110127853798	36.0	33.0	40.0	23.0	41.0
36	33.76921130017571	35.0	33.0	40.0	23.0	41.0
37	33.804522376690734	35.0	33.0	40.0	23.0	41.0
38	33.73704725401117	35.0	33.0	40.0	23.0	41.0
39	33.64862632276939	35.0	32.0	40.0	22.0	41.0
40	33.548893076836706	35.0	32.0	40.0	22.0	41.0
41	33.568797360985926	35.0	32.0	40.0	22.0	41.0
42	33.29249052400938	35.0	32.0	40.0	21.0	41.0
43	33.3489385980086	35.0	32.0	40.0	21.0	41.0
44	33.13846205203373	35.0	32.0	40.0	20.0	41.0
45	32.95056582816668	35.0	31.0	39.0	20.0	41.0
46	33.02864799084721	35.0	32.0	39.0	20.0	41.0
47	32.874709347317435	35.0	31.0	39.0	20.0	41.0
48	32.940832794666136	35.0	32.0	39.0	20.0	41.0
49	32.86941735934717	35.0	31.0	39.0	20.0	41.0
50	32.330220899073495	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	5.0
14	28.0
15	92.0
16	244.0
17	712.0
18	1471.0
19	2754.0
20	4729.0
21	7393.0
22	10696.0
23	15105.0
24	20598.0
25	27141.0
26	33288.0
27	39333.0
28	44127.0
29	51042.0
30	59106.0
31	69512.0
32	82667.0
33	102997.0
34	155706.0
35	203931.0
36	126895.0
37	149891.0
38	193260.0
39	244860.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.65198153661268	25.524267336738315	28.42050637751618	12.403244749132822
2	31.457921746070767	26.576474051414646	28.260818106501333	13.704786096013255
3	28.919175641924394	26.632616830087674	30.629861281815508	13.818346246172428
4	25.142982000928633	29.880218623014898	30.205968129110182	14.770831246946289
5	23.191883878525235	32.847956251119065	29.164018851834655	14.796141018521048
6	21.396771638489064	42.91365847589047	25.91526385588604	9.77430602973443
7	87.2569852238276	4.840600029740498	6.479483607826	1.422931138605899
8	87.05135091664467	4.183031527963656	6.15628328735695	2.60933426803473
9	82.24674296015077	6.155069389439695	8.549604421016214	3.048583229393324
10	46.80838924850615	26.612223345077794	14.172865132906647	12.406522273509408
11	39.206414236594775	24.520859318335624	22.39319974386754	13.879526701202064
12	35.5733998549392	22.974838930920104	25.72644203485708	15.725319179283618
13	22.012339272328894	36.25045141828798	26.563728123283468	15.173481186099655
14	17.442256393448595	37.72964672535863	28.55421723310178	16.273879648090993
15	16.210817651289613	25.19900338980993	42.4766552256788	16.113523733221655
16	18.489668211351766	21.35076490742511	41.497039606454294	18.662527274768827
17	18.868829225806255	22.25038465390253	28.351071416649216	30.529714703641996
18	21.342024842420876	24.473274519979242	34.11981779392262	20.06488284367726
19	27.94708618978687	25.24980501764704	26.74198903243232	20.06111976013377
20	29.47234892281734	24.944448996561633	26.31682128691388	19.266380793707153
21	22.72265163861045	30.30144120030226	28.67269366982584	18.303213491261452
22	23.859709817702882	25.649966466070033	25.313898827678088	25.176424888548997
23	20.37114928820061	31.07997462953353	25.260548014214745	23.28832806805112
24	19.928562107569565	25.588482536561084	36.717923506222746	17.765031849646604
25	18.859482211843396	25.25229350837741	34.75250138839574	21.13572289138345
26	17.76466768027143	32.75849197461739	27.835346886503583	21.6414934586076
27	18.608144648075818	34.067559488584806	27.423228543595624	19.901067319743746
28	17.374156720290607	30.1867278471217	33.93955395321031	18.49956147937739
29	18.22054704309641	25.763162446854032	33.49714885726685	22.51914165278271
30	18.93000968083589	31.503685697551266	30.086581268948187	19.479723352664656
31	26.157800659753516	27.72476078624168	26.89239098438017	19.225047569624632
32	27.877833313607493	25.775240731130715	27.210614323388477	19.13631163187332
33	26.803897826212303	27.038544293617626	26.008673300618785	20.148884579551282
34	20.25789261252075	27.020699994233986	28.3369295059132	24.384477887332064
35	19.74253225175029	26.50970966596564	30.666703083604187	23.081054998679885
36	29.004573360403256	25.689782317755988	25.805648873957942	19.49999544788281
37	21.097849276365103	32.41684040580608	27.714867518216057	18.770442799612766
38	20.882382396052403	32.61470576631858	24.844181028596402	21.658730809032615
39	21.518768379173153	31.25586843774373	26.67346449500329	20.551898688079824
40	25.50672651183399	25.696822925676067	26.089640291699666	22.706810270790278
41	19.333266568947884	24.401654542861216	28.52611549631734	27.73896339187356
42	21.602345250776136	25.237241174203458	25.85772509460817	27.30268848041224
43	21.09305437959195	24.958044653234886	27.83200866723113	26.116892299942034
44	19.882798156089063	27.596087607012688	29.943584094295588	22.577530142602658
45	18.564626407742242	33.181717483468226	25.83290088220031	22.420755226589222
46	21.960566526157983	30.017267697872946	27.11168164313222	20.91048413283685
47	20.695381421899324	27.20132800432148	27.699268929979333	24.404021643799865
48	21.433006491319112	25.604627378860574	31.22370014293648	21.73866598688383
49	22.65151722065933	24.950457791252045	31.547871581739333	20.850153406349293
50	20.948114968271746	30.708157697478434	27.71432126415329	20.629406070096536
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2527.0
1	3025.5
2	3524.0
3	7443.0
4	11362.0
5	9009.5
6	6657.0
7	7456.5
8	8256.0
9	9020.0
10	9784.0
11	10165.0
12	10546.0
13	10961.5
14	11377.0
15	11107.0
16	10837.0
17	10763.5
18	10690.0
19	10389.5
20	10089.0
21	10332.5
22	10576.0
23	11268.0
24	11960.0
25	12570.5
26	13181.0
27	16003.0
28	18825.0
29	21093.5
30	23362.0
31	27775.5
32	32189.0
33	36364.5
34	40540.0
35	44119.5
36	47699.0
37	53249.0
38	58799.0
39	70181.5
40	81564.0
41	100586.5
42	119609.0
43	135360.0
44	151111.0
45	148698.5
46	146286.0
47	142712.5
48	139139.0
49	132152.5
50	125166.0
51	108859.0
52	92552.0
53	84410.5
54	76269.0
55	72139.0
56	68009.0
57	64288.5
58	60568.0
59	59302.5
60	58037.0
61	54175.5
62	50314.0
63	44613.0
64	38912.0
65	33377.5
66	27843.0
67	24175.5
68	20508.0
69	17797.5
70	15087.0
71	12271.0
72	9455.0
73	7816.5
74	6178.0
75	5103.0
76	4028.0
77	3005.5
78	1983.0
79	1535.5
80	1088.0
81	831.0
82	574.0
83	460.5
84	347.0
85	220.0
86	93.0
87	66.0
88	39.0
89	35.0
90	31.0
91	21.5
92	12.0
93	7.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1647585.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.209250430010833
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.81592644095396	19.807022523723994
2	5.832361854081336	2.823942174477947
3	2.127937229762718	1.5454729588400744
4	1.1610569919303142	1.1243327792462419
5	0.7970834229078084	0.9648396099392685
6	0.6156120629203543	0.8942103959384669
7	0.4888326187511704	0.8283989899993566
8	0.41567486554182564	0.805054153389051
9	0.3419601040566627	0.745073801656228
>10	4.527779395072679	26.055304172728672
>50	1.3024018743292762	21.831421084151977
>100	0.569063969325511	21.358167153566196
>500	0.0030417673174721843	0.5005604685549971
>1k	0.001013922439157395	0.36463531986683745
>5k	2.5348060978934873E-4	0.35156441392075255
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5729	0.34772105839759404	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2269	0.13771671871253985	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08521563379127632	0.0
2	0.0	0.0	0.0	0.27015298148502204	0.0
3	0.0	0.0	0.0	0.37703669309929383	0.0
4	0.0	0.0	0.0	0.6331084587441619	0.0
5	0.0	0.0	0.0	0.9819220252672851	0.0
6	0.0	0.0	0.0	1.5589484002342824	0.0
7	0.0	0.0	0.0	1.8229711972371683	0.0
8	0.0	0.0	0.0	2.5167745518440627	0.0
9	0.0	0.0	0.0	2.8178212353232155	0.0
10	0.0	0.0	0.0	3.30860016326927	0.0
11	0.0	0.0	0.0	3.7369847382684354	0.0
12	0.0	0.0	0.0	4.076390595932835	0.0
13	0.0	0.0	0.0	4.239235001532546	0.0
14	0.0	0.0	0.0	4.311886791880237	0.0
15	0.0	0.0	0.0	4.387451937229339	0.0
16	0.0	0.0	0.0	4.528446180318466	0.0
17	0.0	0.0	0.0	4.677209370078024	0.0
18	0.0	0.0	0.0	4.908214143731583	0.0
19	0.0	0.0	0.0	5.0059329260705825	0.0
20	0.0	0.0	0.0	5.115972772269716	0.0
21	0.0	0.0	0.0	5.2467095779580415	0.0
22	0.0	0.0	0.0	5.386247143546464	0.0
23	0.0	0.0	0.0	5.539501755599863	0.0
24	0.0	0.0	0.0	5.657189158677701	0.0
25	0.0	0.0	0.0	5.758974499039503	0.0
26	0.0	0.0	0.0	5.861184703672345	0.0
27	0.0	0.0	0.0	5.965215755181068	0.0
28	0.0	0.0	0.0	6.067972213876674	0.0
29	0.0	0.0	0.0	6.181775143619297	0.0
30	0.0	0.0	0.0	6.349596530679753	0.0
31	0.0	0.0	0.0	6.487191859600567	0.0
32	0.0	0.0	0.0	6.618110749976481	0.0
33	0.0	0.0	0.0	6.744234743579239	0.0
34	0.0	0.0	0.0	6.8710870759323495	0.0
35	0.0	0.0	0.0	7.036662751845883	0.0
36	0.0	0.0	0.0	7.17031291253562	0.0
37	0.0	0.0	0.0	7.308636580206787	0.0
38	0.0	0.0	0.0	7.456671431215992	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACGC	25	4.4436827E-5	44.0	23
CCGTATC	25	4.4436827E-5	44.0	23
TACGATA	20	7.857478E-4	44.0	18
TATTCGA	25	4.4436827E-5	44.0	10
AATGTCG	25	4.4436827E-5	44.0	19
GTTGCGT	25	4.4436827E-5	44.0	11
TATCGCG	35	1.4465877E-7	43.999996	1
CGTAAGG	330	0.0	41.333332	2
ACGCTAG	55	7.8216544E-11	40.0	1
CGTTTTT	4750	0.0	39.924213	1
ACTAACG	50	1.3478711E-9	39.600002	1
CGGTCTA	145	0.0	39.448277	31
ATGTACG	190	0.0	39.36842	1
CGGGTAT	180	0.0	39.11111	6
CCACGAA	45	2.3519533E-8	39.11111	14
GCGATCG	40	4.1259955E-7	38.5	9
ATGGGCG	690	0.0	38.26087	5
ACGTAGG	230	0.0	38.26087	2
AGGGCGA	1680	0.0	38.107143	6
AGGGATC	1340	0.0	38.08955	6
>>END_MODULE