FastQCFastQC Report
Thu 26 May 2016
SRR1546655_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546655_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1713719
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42500.2479986508873392No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA20420.1191560576733992No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT750.044.028
TACGCGG502.7284841E-1144.02
GTGCGAT254.4437584E-544.035
ACCGTAT351.4466423E-743.9999969
CGACTAA351.4466423E-743.99999613
TAATGCG351.4466423E-743.9999961
TACACCG351.4466423E-743.9999961
TAGTACG850.041.4117621
CGTTTTT48700.041.2895281
CGAACGG700.040.857142
CATACGA4150.040.8192818
ACATACG4250.039.8588217
GCGATGT3700.039.8378379
CCAACGG950.039.368422
GCGTAAG900.039.111111
TGCGGGC3250.038.5846144
CCGTATC404.1261228E-738.537
CGCTATC404.1261228E-738.530
AGTAGCG404.1261228E-738.51
ATCGCTA404.1261228E-738.528
Produced by FastQC (version 0.11.5)