##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546655_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1713719
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.953160348925348	31.0	31.0	33.0	30.0	34.0
2	31.327944079513617	31.0	31.0	34.0	30.0	34.0
3	31.486641625610734	31.0	31.0	34.0	30.0	34.0
4	35.250685789210486	37.0	35.0	37.0	33.0	37.0
5	35.03409193689281	37.0	35.0	37.0	32.0	37.0
6	35.10260900415996	37.0	35.0	37.0	32.0	37.0
7	35.676812242847284	37.0	35.0	37.0	35.0	37.0
8	35.72916796744391	37.0	35.0	37.0	35.0	37.0
9	37.497719287701194	39.0	37.0	39.0	35.0	39.0
10	36.705406195531474	39.0	37.0	39.0	32.0	39.0
11	36.27526158022406	38.0	35.0	39.0	31.0	39.0
12	35.73180375545816	37.0	35.0	39.0	30.0	39.0
13	35.52237560533553	37.0	35.0	39.0	30.0	39.0
14	36.43488168130248	38.0	35.0	40.0	30.0	41.0
15	36.67945094849272	38.0	35.0	40.0	31.0	41.0
16	36.77171811714756	38.0	35.0	40.0	31.0	41.0
17	36.690564205683664	38.0	35.0	40.0	31.0	41.0
18	36.618493463630855	38.0	35.0	40.0	31.0	41.0
19	36.59833438270802	38.0	35.0	40.0	31.0	41.0
20	36.465333581526494	38.0	35.0	40.0	31.0	41.0
21	36.34726463323334	38.0	34.0	40.0	30.0	41.0
22	36.25963182995579	38.0	34.0	40.0	30.0	41.0
23	36.076972945973054	38.0	34.0	40.0	30.0	41.0
24	36.05811396150711	38.0	34.0	40.0	30.0	41.0
25	35.93226135673351	38.0	34.0	40.0	30.0	41.0
26	35.81540205833045	38.0	34.0	40.0	30.0	41.0
27	35.62259973776331	37.0	34.0	40.0	29.0	41.0
28	35.617458288085736	37.0	34.0	40.0	29.0	41.0
29	35.542629217508825	37.0	34.0	40.0	29.0	41.0
30	35.52235576544346	37.0	34.0	40.0	29.0	41.0
31	35.4317335572518	37.0	34.0	40.0	29.0	41.0
32	35.24707026064367	37.0	34.0	40.0	27.0	41.0
33	35.057037355599135	37.0	34.0	40.0	26.0	41.0
34	34.99809070215129	37.0	34.0	40.0	26.0	41.0
35	34.780196169850484	37.0	33.0	40.0	25.0	41.0
36	34.56667633375133	37.0	33.0	40.0	24.0	41.0
37	34.63981201118737	37.0	33.0	40.0	24.0	41.0
38	34.634106875164484	37.0	33.0	40.0	24.0	41.0
39	34.53349353073637	37.0	33.0	40.0	24.0	41.0
40	34.524657776449935	37.0	33.0	40.0	24.0	41.0
41	34.56009882600356	37.0	33.0	40.0	24.0	41.0
42	34.31219237226173	37.0	33.0	40.0	23.0	41.0
43	34.3666061938976	37.0	33.0	40.0	23.0	41.0
44	34.18376408267633	37.0	33.0	40.0	23.0	41.0
45	34.012408685437926	36.0	33.0	40.0	23.0	41.0
46	34.09200224774307	37.0	33.0	40.0	23.0	41.0
47	33.93772257878917	36.0	33.0	40.0	23.0	41.0
48	34.00922788391796	36.0	33.0	40.0	23.0	41.0
49	33.92915758067688	36.0	33.0	40.0	23.0	41.0
50	33.41810121729409	36.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	2.0
14	17.0
15	53.0
16	139.0
17	437.0
18	917.0
19	1974.0
20	3267.0
21	5276.0
22	8036.0
23	11678.0
24	16587.0
25	22394.0
26	28795.0
27	34502.0
28	39732.0
29	46523.0
30	55546.0
31	66094.0
32	81244.0
33	100336.0
34	146722.0
35	191414.0
36	135978.0
37	164326.0
38	228004.0
39	323722.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.399207804780133	25.917259480696657	30.120515673806498	13.56301704071671
2	28.186359607380208	28.34577897543296	30.139305218650197	13.328556198536635
3	27.33470306392121	27.011896349401503	31.670478065540497	13.982922521136778
4	23.497959700510997	30.578058596537705	31.528272721490513	14.395708981460789
5	22.228790134205198	33.6822431215386	30.096707803321316	13.99225894093489
6	20.59690065874277	43.18292555547321	26.259731029416137	9.960442756367877
7	90.17032547342943	3.3338604520344353	5.298184824933376	1.1976292496027645
8	90.70279316504048	2.6314699200977523	4.7053805203770285	1.9603563944847433
9	85.2903539028277	5.3747434672778915	6.903348798723711	2.4315538311706875
10	40.78842564037628	37.14517957728192	11.767273397797421	10.299121384544375
11	31.86794334427056	24.199825058834033	29.66139723023436	14.270834366661045
12	31.44021861226957	24.34745719689167	28.624762869525284	15.58756132131347
13	20.938788681224867	34.880455897378745	29.49083251104761	14.689922910348779
14	16.970985324898656	36.93166732702386	29.39315021890987	16.704197129167618
15	15.016931013777638	26.974725728080273	41.683496535896495	16.324846722245596
16	16.688383568134565	24.275566764446214	40.867026624551634	18.16902304286759
17	17.53134557065657	24.342964044863834	30.495197870829465	27.63049251365014
18	19.489251154944306	25.252214627952423	35.06584218299499	20.192692034108276
19	24.618621839403076	26.98966399975725	28.641918540904314	19.749795619935355
20	26.144134481790772	26.70618695363709	28.206491262569884	18.943187302002254
21	22.064410793134698	29.634088202324886	30.053701919626263	18.247799084914153
22	21.96270216995902	26.20791389953662	28.216061092862947	23.61332283764141
23	18.510560949607257	31.00642520739981	28.172588388177992	22.31042545481494
24	18.337078599233596	26.137248872189666	37.16431923786806	18.36135329070869
25	17.17183505580553	28.159634105708115	34.5637178557278	20.10481298275855
26	17.58888125766243	33.5623284797566	29.20478794948297	19.644002313098007
27	17.794515903715837	33.48244373785901	29.800509885226223	18.922530473198933
28	16.59344384931252	30.181552518236654	35.80406122590693	17.420942406543897
29	16.961882315595496	26.24275041590833	35.190600092547264	21.604767175948915
30	19.250413865983862	30.678250051496192	31.798737132517058	18.272598950002887
31	23.939805767456626	28.857006312003307	28.028340702297168	19.174847218242896
32	25.504356315125175	27.218406284810985	29.098352763784497	18.178884636279342
33	24.18237762433631	28.126489815424815	27.909884876108627	19.781247684130246
34	19.315768804570645	27.95685873821788	29.789481239339704	22.937891217871776
35	19.43825096179712	26.94409060061772	31.967784683486617	21.649873754098543
36	25.242236329293195	26.03612377525137	29.325577880621033	19.396062014834403
37	19.86883497236128	31.315168939598614	30.68869517114533	18.127300916894775
38	19.610741317567232	31.32316324905075	28.012001967650473	21.054093465731547
39	20.049319637583523	31.230149166812062	29.206071707205204	19.514459488399208
40	23.12578666630877	26.85597813877304	28.42805617490382	21.590179020014368
41	18.00032560764046	26.190057996672735	30.084220341841338	25.72539605384547
42	20.48585561576898	26.643866351484697	28.723845624632744	24.146432408113583
43	20.247485147798443	26.349419012101748	29.673359518100696	23.729736321999116
44	18.593304970068022	29.3291957432928	31.122196812896398	20.95530247374278
45	17.919098755396888	32.93626317966948	27.79959841724343	21.345039647690196
46	20.669432970049346	31.376497547147466	28.45238921900265	19.50168026380054
47	19.955371913365028	28.15753341125354	29.796658612059503	22.090436063321935
48	20.52436834743619	26.321409752707414	32.272210321528796	20.882011578327603
49	20.645041573326782	25.885982474372987	32.877385382317634	20.591590569982593
50	19.30748273199982	30.753875051860895	29.693374468042894	20.245267748096392
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1467.0
1	1862.5
2	2258.0
3	5764.5
4	9271.0
5	7613.0
6	5955.0
7	6497.0
8	7039.0
9	7648.0
10	8257.0
11	8683.0
12	9109.0
13	9222.0
14	9335.0
15	9103.5
16	8872.0
17	9157.0
18	9442.0
19	9088.5
20	8735.0
21	9744.0
22	10753.0
23	12098.0
24	13443.0
25	16228.5
26	19014.0
27	22831.5
28	26649.0
29	30361.5
30	34074.0
31	41073.0
32	48072.0
33	54756.5
34	61441.0
35	68384.0
36	75327.0
37	81325.0
38	87323.0
39	97392.5
40	107462.0
41	126963.5
42	146465.0
43	157905.0
44	169345.0
45	169327.5
46	169310.0
47	163082.0
48	156854.0
49	143366.0
50	129878.0
51	114827.0
52	99776.0
53	87267.0
54	74758.0
55	64309.5
56	53861.0
57	48560.0
58	43259.0
59	38677.5
60	34096.0
61	30103.5
62	26111.0
63	21597.0
64	17083.0
65	13918.5
66	10754.0
67	8746.0
68	6738.0
69	5775.5
70	4813.0
71	3999.0
72	3185.0
73	2447.5
74	1710.0
75	1397.0
76	1084.0
77	962.5
78	841.0
79	557.0
80	273.0
81	172.0
82	71.0
83	60.0
84	49.0
85	56.5
86	64.0
87	52.0
88	40.0
89	21.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1713719.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.797144134008864
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.91041693782984	18.445264368744592
2	6.3853492788295	2.911354557109313
3	2.196799779238862	1.5024228360260157
4	1.1752594112846055	1.0717023277562223
5	0.840476176319989	0.9580228266383719
6	0.6195503821168428	0.847438761563876
7	0.4913610337689594	0.7841139816066593
8	0.41369644240754855	0.7544877940233266
9	0.3511610164242979	0.7204921475102797
>10	4.4927527839787995	24.297503790790813
>50	1.417264814179112	22.723647154683597
>100	0.7012626882325625	23.969168708570546
>500	0.0036160875249211535	0.5161700592362676
>1k	0.0010331678642631866	0.4982106857401391
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4250	0.2479986508873392	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2042	0.1191560576733992	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04393952567486268	0.0
2	0.0	0.0	0.0	0.23113474262700012	0.0
3	0.0	0.0	0.0	0.3427049592144336	0.0
4	0.0	0.0	0.0	0.5821257744122578	0.0
5	0.0	0.0	0.0	0.94688802539973	0.0
6	0.0	0.0	0.0	1.5291888576832025	0.0
7	0.0	0.0	0.0	1.7998283265809623	0.0
8	0.0	0.0	0.0	2.489031165552812	0.0
9	0.0	0.0	0.0	2.822049589226705	0.0
10	0.0	0.0	0.0	3.347806729107864	0.0
11	0.0	0.0	0.0	3.8026654311471133	0.0
12	0.0	0.0	0.0	4.177872801783723	0.0
13	0.0	0.0	0.0	4.358999345867088	0.0
14	0.0	0.0	0.0	4.436725040686367	0.0
15	0.0	0.0	0.0	4.524370681541139	0.0
16	0.0	0.0	0.0	4.695460574341534	0.0
17	0.0	0.0	0.0	4.882947554412363	0.0
18	0.0	0.0	0.0	5.153178554943955	0.0
19	0.0	0.0	0.0	5.268191576331942	0.0
20	0.0	0.0	0.0	5.396625701179715	0.0
21	0.0	0.0	0.0	5.5556949534900415	0.0
22	0.0	0.0	0.0	5.725792851686887	0.0
23	0.0	0.0	0.0	5.918239804775462	0.0
24	0.0	0.0	0.0	6.067797579416462	0.0
25	0.0	0.0	0.0	6.195298062284423	0.0
26	0.0	0.0	0.0	6.316613167036136	0.0
27	0.0	0.0	0.0	6.4417211923308315	0.0
28	0.0	0.0	0.0	6.570038611931127	0.0
29	0.0	0.0	0.0	6.708042567071964	0.0
30	0.0	0.0	0.0	6.885376190612346	0.0
31	0.0	0.0	0.0	7.041644516983239	0.0
32	0.0	0.0	0.0	7.184258329399394	0.0
33	0.0	0.0	0.0	7.325063210479664	0.0
34	0.0	0.0	0.0	7.473920753635806	0.0
35	0.0	0.0	0.0	7.65247978227469	0.0
36	0.0	0.0	0.0	7.807289293052128	0.0
37	0.0	0.0	0.0	7.977562249120188	0.0
38	0.0	0.0	0.0	8.141533121824523	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	75	0.0	44.0	28
TACGCGG	50	2.7284841E-11	44.0	2
GTGCGAT	25	4.4437584E-5	44.0	35
ACCGTAT	35	1.4466423E-7	43.999996	9
CGACTAA	35	1.4466423E-7	43.999996	13
TAATGCG	35	1.4466423E-7	43.999996	1
TACACCG	35	1.4466423E-7	43.999996	1
TAGTACG	85	0.0	41.411762	1
CGTTTTT	4870	0.0	41.289528	1
CGAACGG	70	0.0	40.85714	2
CATACGA	415	0.0	40.81928	18
ACATACG	425	0.0	39.85882	17
GCGATGT	370	0.0	39.837837	9
CCAACGG	95	0.0	39.36842	2
GCGTAAG	90	0.0	39.11111	1
TGCGGGC	325	0.0	38.584614	4
CCGTATC	40	4.1261228E-7	38.5	37
CGCTATC	40	4.1261228E-7	38.5	30
AGTAGCG	40	4.1261228E-7	38.5	1
ATCGCTA	40	4.1261228E-7	38.5	28
>>END_MODULE