FastQCFastQC Report
Thu 26 May 2016
SRR1546654_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546654_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1927515
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73400.38080118702059385No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA28070.14562791988648596No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACC207.8578095E-444.00000439
CCGACAA207.8578095E-444.00000423
ATCGTAG551.8189894E-1244.01
CGTTTTT74900.041.503341
TAGCGCG557.8216544E-1140.01
CGAATAT1000.039.60000214
TAGTACG452.3523171E-839.111111
CGTTAGG2650.039.0188672
CGACAGG1200.038.52
AGGGATC27750.038.3711746
ATAGCGC357.292774E-637.71428723
CAATTCG357.292774E-637.7142871
AACGGGA6950.037.6690674
TAAGGGA30900.037.4498374
TTAATCG5700.037.438620
ATCGTTG5700.037.438623
ACGCGTA651.0913936E-1137.23076623
AAATGCG950.037.052631
GCGGCTA4300.036.83720824
TAGGGCG8450.036.710065
Produced by FastQC (version 0.11.5)