##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546654_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1927515
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.94663128432204	31.0	31.0	33.0	30.0	34.0
2	31.30833793770736	31.0	31.0	34.0	30.0	34.0
3	31.46007942869446	31.0	31.0	34.0	30.0	34.0
4	35.23203917998044	37.0	35.0	37.0	33.0	37.0
5	35.02466751231508	35.0	35.0	37.0	32.0	37.0
6	35.10123345343616	37.0	35.0	37.0	32.0	37.0
7	35.675814714801184	37.0	35.0	37.0	35.0	37.0
8	35.7208436769623	37.0	35.0	37.0	35.0	37.0
9	37.47666088201648	39.0	37.0	39.0	35.0	39.0
10	36.70958565821797	39.0	37.0	39.0	32.0	39.0
11	36.30063890553381	38.0	35.0	39.0	31.0	39.0
12	35.81518794925072	37.0	35.0	39.0	31.0	39.0
13	35.647222460006795	37.0	35.0	39.0	30.0	39.0
14	36.59517254081032	38.0	35.0	40.0	31.0	41.0
15	36.797246714033356	38.0	35.0	40.0	31.0	41.0
16	36.84862685893495	38.0	35.0	40.0	31.0	41.0
17	36.749846823500725	38.0	35.0	40.0	31.0	41.0
18	36.68065462525583	38.0	35.0	40.0	31.0	41.0
19	36.64192081514281	38.0	35.0	40.0	31.0	41.0
20	36.50711096930504	38.0	35.0	40.0	31.0	41.0
21	36.39839638083232	38.0	35.0	40.0	30.0	41.0
22	36.33675120556779	38.0	35.0	40.0	30.0	41.0
23	36.14090162722469	38.0	34.0	40.0	30.0	41.0
24	36.086681037501656	38.0	34.0	40.0	30.0	41.0
25	35.93737843804069	38.0	34.0	40.0	30.0	41.0
26	35.830047496387834	38.0	34.0	40.0	30.0	41.0
27	35.647429981089644	37.0	34.0	40.0	29.0	41.0
28	35.60006381273298	37.0	34.0	40.0	29.0	41.0
29	35.48060222618242	37.0	34.0	40.0	29.0	41.0
30	35.431170704248736	37.0	34.0	40.0	28.0	41.0
31	35.36587471433426	37.0	34.0	40.0	27.0	41.0
32	35.1454826551285	37.0	34.0	40.0	27.0	41.0
33	34.93055410723133	37.0	34.0	40.0	25.0	41.0
34	34.8543710425081	37.0	34.0	40.0	25.0	41.0
35	34.63071156385294	37.0	33.0	40.0	24.0	41.0
36	34.42235468984677	37.0	33.0	40.0	24.0	41.0
37	34.49252119957562	37.0	33.0	40.0	24.0	41.0
38	34.45549009994734	37.0	33.0	40.0	24.0	41.0
39	34.37046974991115	37.0	33.0	40.0	23.0	41.0
40	34.37250293772033	37.0	33.0	40.0	23.0	41.0
41	34.39105584132938	37.0	33.0	40.0	23.0	41.0
42	34.15420269102964	37.0	33.0	40.0	23.0	41.0
43	34.19767213225319	37.0	33.0	40.0	23.0	41.0
44	33.998027512107555	37.0	33.0	40.0	23.0	41.0
45	33.80665053190248	36.0	33.0	40.0	22.0	41.0
46	33.89684593894211	37.0	33.0	40.0	23.0	41.0
47	33.74216543061922	36.0	33.0	40.0	22.0	41.0
48	33.78070002049271	36.0	33.0	40.0	22.0	41.0
49	33.70108403825651	36.0	32.0	40.0	22.0	41.0
50	33.196820777010814	35.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	3.0
14	15.0
15	52.0
16	159.0
17	489.0
18	1196.0
19	2345.0
20	4070.0
21	6170.0
22	9540.0
23	13797.0
24	19660.0
25	26716.0
26	34298.0
27	40547.0
28	45817.0
29	52766.0
30	62176.0
31	74588.0
32	90808.0
33	112622.0
34	160606.0
35	205874.0
36	158108.0
37	190752.0
38	258441.0
39	355898.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.012980443731955	24.684684684684687	29.136115672251577	14.166219199331781
2	29.47131410131698	26.67211409509135	30.259271652879484	13.597300150712185
3	27.451615162527922	26.678132206493853	32.76508872823298	13.105163902745245
4	23.317120748736066	30.08593966843319	32.47632314145415	14.12061644137659
5	22.077701081444243	33.7670005162087	30.916490922249633	13.238807480097432
6	20.721758326134946	42.51572620705935	26.781840867645645	9.980674599160059
7	88.80039844047907	3.6188045229220007	6.292194872672846	1.2886021639260914
8	89.16900776388252	2.9275517959652713	5.806491778274099	2.096948661878118
9	83.40702925787866	6.115023748193918	8.007304742116144	2.4706422518112703
10	38.889710326508485	37.71690492680991	12.725452201409587	10.667932545272022
11	30.02913077200437	25.59030669022031	29.878729867212446	14.501832670562875
12	29.533985468336173	24.095272929134147	29.63899113625575	16.731750466273933
13	20.917295066445657	32.73074398902214	30.469334868989346	15.882626075542863
14	17.436699584698435	34.933113360985516	30.050868605432385	17.579318448883665
15	16.135853676884487	26.538574278280585	40.71153791280483	16.6140341320301
16	18.423358573085032	24.287022409682933	39.62869290251956	17.660926114712467
17	18.20105161308732	24.34528395369167	31.093091363750737	26.360573069470277
18	19.59569705034721	25.76011081625824	34.66079382002215	19.9833983133724
19	23.945805869215025	27.495350230737504	29.410614184584816	19.148229715462655
20	25.77904711506785	26.515331917001944	28.923769724230418	18.78185124369979
21	21.138771942111994	30.418699724775163	30.4230057872442	18.019522545868643
22	21.50758878659829	26.776185918138122	28.416225035862237	23.300000259401354
23	18.799283014658773	31.190626272687894	28.52916838520063	21.480922327452703
24	18.3494291873215	26.830089519407114	36.67686113986143	18.143620153409962
25	17.55016173674394	27.971714876408228	34.17571328887194	20.302410097975894
26	17.91545072282187	32.94812232330228	29.802465869266904	19.333961084608937
27	18.074567513093285	32.455311631816095	29.819534478330905	19.650586376759712
28	17.038777908343125	30.015382500265886	35.148779646332194	17.797059945058795
29	17.596957740925493	26.534631377706532	34.585256145866566	21.283154735501412
30	18.605510203552242	30.607699550976257	31.90330555144837	18.883484694023135
31	23.397794569692067	28.037550939940804	29.29154896330249	19.27310552706464
32	24.23555718113737	27.704583362515983	30.066069524750784	17.993789931595863
33	23.25014331924784	28.26447524403182	29.062912610278	19.422468826442334
34	19.598031662529213	28.152465739566228	30.05953261064116	22.189969987263396
35	19.189578291219522	26.50443706015258	32.712326492919644	21.593658155708255
36	24.41236514372132	26.699454997756177	29.524543259066725	19.363636599455774
37	19.54760403939788	30.701550960692913	30.493511075140788	19.257333924768417
38	18.971317992337283	30.620047055405536	29.76921061574099	20.63942433651619
39	19.354816953434863	30.83825547401706	29.56843396808845	20.238493604459627
40	22.447140489179073	27.16663683551101	29.469135129947112	20.91708754536281
41	17.271460922483094	26.49219331626472	30.693042596296266	25.543303164955915
42	20.296755148468364	27.219139669470792	28.528701462764232	23.95540371929661
43	20.17276130146847	27.579655670643287	29.419226309522884	22.828356718365356
44	19.897795866698832	29.435776115879776	29.984617499734114	20.681810517687282
45	18.21189458966597	32.46532452406336	27.620796725317316	21.70198416095335
46	20.22204755864416	31.352337076494862	28.41586187396726	20.009753490893715
47	20.28279935564704	27.83552916579119	29.447086014894825	22.434585463666952
48	20.136963914677708	27.02962104056259	31.770336417615425	21.06307862714428
49	20.432110774754022	26.220911380715584	31.678248937102953	21.66872890742744
50	19.103820203733825	30.74549354998534	29.743529881738922	20.40715636454191
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2318.0
1	2564.5
2	2811.0
3	8839.0
4	14867.0
5	11749.0
6	8631.0
7	9226.5
8	9822.0
9	10582.0
10	11342.0
11	11675.5
12	12009.0
13	12141.0
14	12273.0
15	12133.5
16	11994.0
17	11986.0
18	11978.0
19	12303.0
20	12628.0
21	12963.5
22	13299.0
23	14632.0
24	15965.0
25	19591.5
26	23218.0
27	26483.5
28	29749.0
29	36437.5
30	43126.0
31	49212.0
32	55298.0
33	63418.0
34	71538.0
35	79736.5
36	87935.0
37	91318.0
38	94701.0
39	103927.5
40	113154.0
41	130882.0
42	148610.0
43	161648.5
44	174687.0
45	175312.0
46	175937.0
47	172582.5
48	169228.0
49	160045.5
50	150863.0
51	130147.0
52	109431.0
53	96404.0
54	83377.0
55	73550.0
56	63723.0
57	58013.5
58	52304.0
59	47589.5
60	42875.0
61	37809.5
62	32744.0
63	28165.0
64	23586.0
65	19628.0
66	15670.0
67	12684.0
68	9698.0
69	8127.5
70	6557.0
71	5149.0
72	3741.0
73	3230.5
74	2720.0
75	2217.5
76	1715.0
77	1233.5
78	752.0
79	569.5
80	387.0
81	271.0
82	155.0
83	99.5
84	44.0
85	38.0
86	32.0
87	23.0
88	14.0
89	9.0
90	4.0
91	3.5
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1927515.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.744631422093672
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.00279111749381	19.5489494751904
2	6.772153102259939	3.351488648988208
3	2.6000124647342693	1.9300905039809642
4	1.4393202777929293	1.4246179908932604
5	0.9959320549153972	1.2321985810164926
6	0.7534890206682332	1.118688485821785
7	0.6136856070548926	1.0629796908931988
8	0.507364919992492	1.0043646353371407
9	0.43434981098469877	0.9673043382965182
>10	5.143544771520057	29.294957941435012
>50	1.2621633924460431	21.58573727184984
>100	0.47179922188073625	16.3269208793701
>500	0.0021213989103255767	0.35296450632533716
>1k	0.0010606994551627883	0.4134367120491308
>5k	2.1213989103255763E-4	0.3853003385526435
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7340	0.38080118702059385	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2807	0.14562791988648596	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06656238732253705	0.0
2	0.0	0.0	0.0	0.23844172418891682	0.0
3	0.0	0.0	0.0	0.3451075607712521	0.0
4	0.0	0.0	0.0	0.5465067716723346	0.0
5	0.0	0.0	0.0	0.870706583346952	0.0
6	0.0	0.0	0.0	1.5054616954991271	0.0
7	0.0	0.0	0.0	1.810050764844891	0.0
8	0.0	0.0	0.0	2.6186566641504734	0.0
9	0.0	0.0	0.0	3.0440230037120335	0.0
10	0.0	0.0	0.0	3.6982850976516395	0.0
11	0.0	0.0	0.0	4.175791109278008	0.0
12	0.0	0.0	0.0	4.574646630506118	0.0
13	0.0	0.0	0.0	4.809819897640226	0.0
14	0.0	0.0	0.0	4.922503845625067	0.0
15	0.0	0.0	0.0	5.023929774865565	0.0
16	0.0	0.0	0.0	5.204369356399302	0.0
17	0.0	0.0	0.0	5.407013693797454	0.0
18	0.0	0.0	0.0	5.687478437262485	0.0
19	0.0	0.0	0.0	5.829370977657762	0.0
20	0.0	0.0	0.0	5.973131207798643	0.0
21	0.0	0.0	0.0	6.146774473869205	0.0
22	0.0	0.0	0.0	6.335099856551052	0.0
23	0.0	0.0	0.0	6.544903671307357	0.0
24	0.0	0.0	0.0	6.710194213793407	0.0
25	0.0	0.0	0.0	6.855355211243492	0.0
26	0.0	0.0	0.0	6.998337237323704	0.0
27	0.0	0.0	0.0	7.142979432066676	0.0
28	0.0	0.0	0.0	7.293328456587886	0.0
29	0.0	0.0	0.0	7.449591831970179	0.0
30	0.0	0.0	0.0	7.670031102222292	0.0
31	0.0	0.0	0.0	7.855347429202886	0.0
32	0.0	0.0	0.0	8.029665138792694	0.0
33	0.0	0.0	0.0	8.187951844732726	0.0
34	0.0	0.0	0.0	8.358534174831325	0.0
35	0.0	0.0	0.0	8.564706370637841	0.0
36	0.0	0.0	0.0	8.753654316568223	0.0
37	0.0	0.0	0.0	8.934249538914093	0.0
38	0.0	0.0	0.0	9.117750056419794	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGACC	20	7.8578095E-4	44.000004	39
CCGACAA	20	7.8578095E-4	44.000004	23
ATCGTAG	55	1.8189894E-12	44.0	1
CGTTTTT	7490	0.0	41.50334	1
TAGCGCG	55	7.8216544E-11	40.0	1
CGAATAT	100	0.0	39.600002	14
TAGTACG	45	2.3523171E-8	39.11111	1
CGTTAGG	265	0.0	39.018867	2
CGACAGG	120	0.0	38.5	2
AGGGATC	2775	0.0	38.371174	6
ATAGCGC	35	7.292774E-6	37.714287	23
CAATTCG	35	7.292774E-6	37.714287	1
AACGGGA	695	0.0	37.669067	4
TAAGGGA	3090	0.0	37.449837	4
TTAATCG	570	0.0	37.4386	20
ATCGTTG	570	0.0	37.4386	23
ACGCGTA	65	1.0913936E-11	37.230766	23
AAATGCG	95	0.0	37.05263	1
GCGGCTA	430	0.0	36.837208	24
TAGGGCG	845	0.0	36.71006	5
>>END_MODULE