FastQCFastQC Report
Thu 26 May 2016
SRR1546653_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546653_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1712007
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67380.39357315711910057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTA800.044.00000438
GTCGTTA207.8575616E-444.00000431
CCGCTAA454.802132E-1044.018
TCGCGAT254.4437555E-544.039
ATTTACG750.041.0666661
CTAACGG750.041.0666662
CGGTCTA1300.040.61538731
CGTTAGG1900.040.5263182
AGGGCGA20250.039.7629666
CGTAAGG4150.039.7590372
TTACGCG501.3478711E-939.6000021
CGTTTTT54700.039.4954261
TAACGGG2750.039.23
TAGCACG452.3521352E-839.111111
CGAAAGG2550.038.8235322
GCATATC9250.038.52972828
TGTTACG800.038.5000041
TAGGGCG8200.038.3658565
GCGATAA1550.038.3225829
GTTACGG1150.038.260872
Produced by FastQC (version 0.11.5)