##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546653_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1712007
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.978260018796654	31.0	31.0	33.0	30.0	34.0
2	31.34235841325415	31.0	31.0	34.0	30.0	34.0
3	31.51352418535672	31.0	31.0	34.0	30.0	34.0
4	35.29576456171032	37.0	35.0	37.0	33.0	37.0
5	35.08835828358178	37.0	35.0	37.0	32.0	37.0
6	35.16469967704571	37.0	35.0	37.0	32.0	37.0
7	35.64153943295793	37.0	35.0	37.0	35.0	37.0
8	35.66931677265338	37.0	35.0	37.0	35.0	37.0
9	37.446164647691276	39.0	37.0	39.0	35.0	39.0
10	36.69544341816359	39.0	37.0	39.0	32.0	39.0
11	36.30231476857279	38.0	35.0	39.0	32.0	39.0
12	35.581350426721386	37.0	35.0	39.0	30.0	39.0
13	35.29222485655725	37.0	35.0	39.0	30.0	39.0
14	36.17056355493874	38.0	35.0	40.0	30.0	41.0
15	36.44369561573054	38.0	35.0	40.0	30.0	41.0
16	36.57032827552691	38.0	35.0	40.0	31.0	41.0
17	36.476675621069305	38.0	35.0	40.0	31.0	41.0
18	36.42903212428454	38.0	35.0	40.0	31.0	41.0
19	36.370304560670604	38.0	35.0	40.0	31.0	41.0
20	36.22029115535158	38.0	34.0	40.0	30.0	41.0
21	36.09166960181822	38.0	34.0	40.0	30.0	41.0
22	36.01444036151722	38.0	34.0	40.0	30.0	41.0
23	35.836692256515306	37.0	34.0	40.0	30.0	41.0
24	35.7680213924359	37.0	34.0	40.0	30.0	41.0
25	35.60301388954601	37.0	34.0	40.0	29.0	41.0
26	35.47622059956531	37.0	34.0	40.0	29.0	41.0
27	35.287023359133464	36.0	34.0	40.0	29.0	41.0
28	35.26421095240849	36.0	34.0	40.0	28.0	41.0
29	35.132424692188756	36.0	34.0	40.0	27.0	41.0
30	35.09659715176398	36.0	34.0	40.0	27.0	41.0
31	34.99952161410555	36.0	34.0	40.0	27.0	41.0
32	34.811539906086836	36.0	33.0	40.0	26.0	41.0
33	34.594582265142606	36.0	33.0	40.0	25.0	41.0
34	34.47923460593327	36.0	33.0	40.0	25.0	41.0
35	34.26449775030125	36.0	33.0	40.0	24.0	41.0
36	34.013876695597624	36.0	33.0	40.0	23.0	41.0
37	34.06932097824367	36.0	33.0	40.0	23.0	41.0
38	34.03962659031184	36.0	33.0	40.0	23.0	41.0
39	33.96153345167397	36.0	33.0	40.0	23.0	41.0
40	33.869210815142694	35.0	33.0	40.0	23.0	41.0
41	33.87647538824316	35.0	33.0	40.0	23.0	41.0
42	33.650421990097	35.0	33.0	40.0	23.0	41.0
43	33.70458298359761	35.0	33.0	40.0	23.0	41.0
44	33.51167956673074	35.0	32.0	40.0	22.0	41.0
45	33.3245284627925	35.0	32.0	39.0	22.0	41.0
46	33.39012048432045	35.0	32.0	39.0	23.0	41.0
47	33.246270605201964	35.0	32.0	39.0	22.0	41.0
48	33.28038904046537	35.0	32.0	39.0	22.0	41.0
49	33.20559612197847	35.0	32.0	39.0	22.0	41.0
50	32.69638675542799	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	5.0
14	9.0
15	58.0
16	191.0
17	526.0
18	1173.0
19	2359.0
20	3980.0
21	6324.0
22	9496.0
23	13484.0
24	18648.0
25	24764.0
26	31532.0
27	37698.0
28	43478.0
29	50177.0
30	59314.0
31	70110.0
32	84876.0
33	106175.0
34	161500.0
35	210308.0
36	137939.0
37	163231.0
38	211012.0
39	263639.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.42883527929501	24.343533642093753	29.13253275249459	13.095098326116657
2	30.88603025571741	26.660580242954612	29.148946236785246	13.304443264542726
3	28.847720832917155	26.25585058939596	30.9340440780908	13.962384499596089
4	25.617652264272284	29.39859474873642	30.023008083494986	14.960744903496307
5	22.88325923901012	33.36586824703404	29.757997484823367	13.992875029132474
6	21.393195238103583	43.11512744982935	25.86151808958725	9.630159222479815
7	88.78725379043428	4.0520862356287095	5.938059832699282	1.2226001412377403
8	89.06622461239937	3.3611427990656577	5.691507102482642	1.881125486052335
9	84.03908395234365	5.560491283037979	7.774267278112765	2.6261574865056043
10	47.80541201058173	25.915723475429715	13.893868424603403	12.38499608938515
11	39.1447581697972	24.810996684008884	21.86790124105801	14.176343905135901
12	36.764802947651496	22.06013176347994	25.33733798985635	15.837727299012212
13	22.658318569959118	34.222698855787385	26.696094116437607	16.422888457815883
14	18.618615461268558	36.126370978623335	28.181602061206522	17.07341149890158
15	17.063072756127752	24.603053609009777	41.16793914978151	17.16593448508096
16	19.45657932473407	20.377778829175348	40.687275227262504	19.478366618828076
17	19.799393343601984	21.024914033645892	29.443512789375276	29.732179833376847
18	21.99079793482153	23.74855943930136	32.997820686480836	21.262821939396275
19	28.62061895774959	24.74610208953585	26.360523058608987	20.272755894105572
20	30.330366639856027	24.173791345479312	25.83371446495254	19.66212754971212
21	24.246980298561862	28.696377993781567	27.901755074599578	19.154886633056993
22	24.185765595584595	24.525659065646344	25.43996607490507	25.84860926386399
23	21.872982995980742	29.54672498418523	25.073495610707198	23.50679640912683
24	21.020065922627655	24.2615830425927	36.31252675952844	18.405824275251213
25	20.57351401016468	25.21233850095239	33.297761048874214	20.916386440008715
26	19.619020249333094	31.11284007600436	27.618520251377475	21.649619423285067
27	19.76376264816674	32.77813700528094	27.18119727314199	20.27690307341033
28	18.318616687899056	29.82335936710539	32.943089601853266	18.91493434314229
29	19.479476427374422	25.795864152424606	32.67574256413671	22.048916856064256
30	20.73367690669489	30.31681529339541	29.481129457998712	19.468378341910984
31	27.22611531378084	27.701054960639766	25.994461471243984	19.07836825433541
32	28.559404254772325	26.2004185730549	26.173783167942656	19.066394004230123
33	28.85852686349997	26.744808870524476	24.563392556222023	19.83327170975352
34	21.819420130875635	27.375238535823744	26.727110344759105	24.07823098854152
35	21.74704893145881	25.999251171286097	29.771548831283983	22.48215106597111
36	29.17850219070366	26.503805183039553	24.161583451469532	20.156109174787254
37	21.980809657904434	32.54963326668641	26.383361750273217	19.08619532513594
38	22.039279045003905	31.304486488665056	25.277817205186658	21.378417261144374
39	22.215621781920284	30.98842469686164	25.430678729701455	21.365274791516622
40	25.81402996599897	25.597442066533603	26.70281137869179	21.88571658877563
41	19.10564618018501	25.3462164582271	27.82640491540046	27.72173244618743
42	21.51299615013256	25.923725779158612	25.526122264687	27.037155806021822
43	21.8638708837055	25.315784339666834	26.987681709245347	25.832663067382317
44	20.006285021030873	27.789197123609892	29.19806986770498	23.006447987654255
45	19.50132213244455	32.69180558257063	25.451473037201367	22.35539924778345
46	21.32064880575839	31.30571311916365	26.52255510637515	20.851082968702812
47	21.937819179477653	27.268580093422518	26.941303394203413	23.85229733289642
48	22.27823834832451	25.958538720928125	30.290530354139904	21.472692576607454
49	21.94471167466021	25.594579928703563	30.944616464769126	21.516091931867102
50	21.288113892057684	30.673180658723943	27.37699086510744	20.66171458411093
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1930.0
1	2559.5
2	3189.0
3	8040.5
4	12892.0
5	9853.5
6	6815.0
7	7345.0
8	7875.0
9	8534.0
10	9193.0
11	9465.0
12	9737.0
13	9870.0
14	10003.0
15	9634.0
16	9265.0
17	9117.0
18	8969.0
19	8738.0
20	8507.0
21	8640.5
22	8774.0
23	8999.0
24	9224.0
25	11296.0
26	13368.0
27	16096.0
28	18824.0
29	22302.0
30	25780.0
31	28432.5
32	31085.0
33	36672.5
34	42260.0
35	46643.5
36	51027.0
37	54833.5
38	58640.0
39	70078.0
40	81516.0
41	101630.0
42	121744.0
43	136285.0
44	150826.0
45	148435.5
46	146045.0
47	137436.5
48	128828.0
49	124835.5
50	120843.0
51	109526.5
52	98210.0
53	89120.0
54	80030.0
55	75970.5
56	71911.0
57	72380.5
58	72850.0
59	71883.0
60	70916.0
61	67532.5
62	64149.0
63	56181.5
64	48214.0
65	41460.5
66	34707.0
67	29283.5
68	23860.0
69	20891.5
70	17923.0
71	15499.0
72	13075.0
73	10433.5
74	7792.0
75	6538.0
76	5284.0
77	4150.5
78	3017.0
79	2358.0
80	1699.0
81	1195.0
82	691.0
83	470.5
84	250.0
85	184.0
86	118.0
87	112.0
88	106.0
89	68.0
90	30.0
91	21.5
92	13.0
93	7.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1712007.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.690065274656938
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.58786816276813	17.47956120795888
2	5.96039902383246	2.5856288777985506
3	2.2245008457916304	1.4474870564625009
4	1.2638276203298309	1.096500143234735
5	0.837530261183554	0.908304301728588
6	0.6551916972844412	0.8526690408907677
7	0.5250870729550058	0.7972421021090836
8	0.43070527688672905	0.7473620455849874
9	0.4016112958026051	0.7839877698898254
>10	4.899046144331008	25.269556690468807
>50	1.52346052725683	23.104910354462504
>100	0.6875098658093777	24.023158731026452
>500	0.0024466543263083	0.32860941883432815
>1k	5.437009614018445E-4	0.17771914379448506
>5k	2.7185048070092223E-4	0.39730311575555416
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6738	0.39357315711910057	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.033294256390306816	0.0
2	0.0	0.0	0.0	0.12996442187444326	0.0
3	0.0	0.0	0.0	0.19328191999214955	0.0
4	0.0	0.0	0.0	0.32920426143117404	0.0
5	0.0	0.0	0.0	0.5240632777786539	0.0
6	5.841097612334529E-5	0.0	0.0	0.8515152099261276	0.0
7	5.841097612334529E-5	0.0	0.0	0.9908837989564295	0.0
8	5.841097612334529E-5	0.0	0.0	1.360099579032095	0.0
9	5.841097612334529E-5	0.0	0.0	1.5232414353445984	0.0
10	5.841097612334529E-5	0.0	0.0	1.7877847462072294	0.0
11	5.841097612334529E-5	0.0	0.0	2.032643558116293	0.0
12	5.841097612334529E-5	0.0	0.0	2.2272689305592794	0.0
13	5.841097612334529E-5	0.0	0.0	2.3222451777358386	0.0
14	5.841097612334529E-5	0.0	0.0	2.3686819037538984	0.0
15	5.841097612334529E-5	0.0	0.0	2.419265809076715	0.0
16	5.841097612334529E-5	0.0	0.0	2.526157895382437	0.0
17	5.841097612334529E-5	0.0	0.0	2.6425709707962644	0.0
18	5.841097612334529E-5	0.0	0.0	2.805420772228151	0.0
19	5.841097612334529E-5	0.0	0.0	2.8784344923823326	0.0
20	5.841097612334529E-5	0.0	0.0	2.950922513751404	0.0
21	5.841097612334529E-5	0.0	0.0	3.047651090211664	0.0
22	5.841097612334529E-5	0.0	0.0	3.148643667928928	0.0
23	5.841097612334529E-5	0.0	0.0	3.2693791555758827	0.0
24	5.841097612334529E-5	0.0	0.0	3.3610843880895347	0.0
25	5.841097612334529E-5	0.0	0.0	3.441866768068121	0.0
26	5.841097612334529E-5	0.0	0.0	3.523525312688558	0.0
27	5.841097612334529E-5	0.0	0.0	3.6092142146615056	0.0
28	5.841097612334529E-5	0.0	0.0	3.6936180751597396	0.0
29	5.841097612334529E-5	0.0	0.0	3.785732184506255	0.0
30	5.841097612334529E-5	0.0	0.0	3.9102059746251037	0.0
31	5.841097612334529E-5	0.0	0.0	4.027144748824041	0.0
32	5.841097612334529E-5	0.0	0.0	4.132109272917693	0.0
33	5.841097612334529E-5	0.0	0.0	4.238300427509934	0.0
34	5.841097612334529E-5	0.0	0.0	4.349748569953277	0.0
35	5.841097612334529E-5	0.0	0.0	4.484210636989218	0.0
36	5.841097612334529E-5	0.0	0.0	4.605471823421283	0.0
37	5.841097612334529E-5	0.0	0.0	4.727609174495198	0.0
38	5.841097612334529E-5	0.0	0.0	4.8565221987994205	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTTA	80	0.0	44.000004	38
GTCGTTA	20	7.8575616E-4	44.000004	31
CCGCTAA	45	4.802132E-10	44.0	18
TCGCGAT	25	4.4437555E-5	44.0	39
ATTTACG	75	0.0	41.066666	1
CTAACGG	75	0.0	41.066666	2
CGGTCTA	130	0.0	40.615387	31
CGTTAGG	190	0.0	40.526318	2
AGGGCGA	2025	0.0	39.762966	6
CGTAAGG	415	0.0	39.759037	2
TTACGCG	50	1.3478711E-9	39.600002	1
CGTTTTT	5470	0.0	39.495426	1
TAACGGG	275	0.0	39.2	3
TAGCACG	45	2.3521352E-8	39.11111	1
CGAAAGG	255	0.0	38.823532	2
GCATATC	925	0.0	38.529728	28
TGTTACG	80	0.0	38.500004	1
TAGGGCG	820	0.0	38.365856	5
GCGATAA	155	0.0	38.322582	9
GTTACGG	115	0.0	38.26087	2
>>END_MODULE