FastQCFastQC Report
Thu 26 May 2016
SRR1546652_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546652_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2012282
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64180.31894138097940544No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA38770.19266683297867793No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCCG207.857892E-444.0000041
CCTCGCA207.857892E-444.00000414
TAGCCGT207.857892E-444.00000444
CGCACGA207.857892E-444.00000440
CATACGT302.5286681E-644.037
CGTAAAT454.802132E-1044.016
TAATGCG254.4440363E-544.01
GTTATCG254.4440363E-544.01
TATTGCG1600.042.6250041
TATAGCG900.041.5555571
CGTACAT700.040.85714335
CGGTCTA1400.040.85714331
TATCACG650.040.6153871
CCCGTAA650.040.61538733
CGTTTTT55950.040.22521
CGGTATG557.8216544E-1140.0000046
CGGAATA501.3496901E-939.629
CTAAACG501.3496901E-939.61
TCGATTG900.039.111111
TTACGTC452.352499E-839.1111132
Produced by FastQC (version 0.11.5)