##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546652_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2012282
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.940940683264074	31.0	31.0	33.0	30.0	34.0
2	31.309334874535477	31.0	31.0	34.0	30.0	34.0
3	31.470392320758222	31.0	31.0	34.0	30.0	34.0
4	35.25058863519129	37.0	35.0	37.0	33.0	37.0
5	35.04075373133587	37.0	35.0	37.0	32.0	37.0
6	35.11467030962857	37.0	35.0	37.0	32.0	37.0
7	35.60236835592626	36.0	35.0	37.0	35.0	37.0
8	35.62596147060899	37.0	35.0	37.0	35.0	37.0
9	37.3933882030451	39.0	37.0	39.0	35.0	39.0
10	36.67790846412183	39.0	35.0	39.0	32.0	39.0
11	36.271005753666735	38.0	35.0	39.0	32.0	39.0
12	35.452757615483314	37.0	35.0	39.0	30.0	39.0
13	35.11846947892989	37.0	34.0	39.0	30.0	39.0
14	35.99433578395076	38.0	34.0	40.0	29.0	41.0
15	36.31641589001939	38.0	35.0	40.0	30.0	41.0
16	36.466391887419356	38.0	35.0	40.0	31.0	41.0
17	36.385383857729686	38.0	35.0	40.0	31.0	41.0
18	36.35975077051825	38.0	35.0	40.0	31.0	41.0
19	36.28122748203284	37.0	35.0	40.0	31.0	41.0
20	36.11933267802425	37.0	34.0	40.0	30.0	41.0
21	35.96859485897106	37.0	34.0	40.0	30.0	41.0
22	35.90722373901868	37.0	34.0	40.0	30.0	41.0
23	35.737305208713295	37.0	34.0	40.0	30.0	41.0
24	35.6911854302727	37.0	34.0	40.0	30.0	41.0
25	35.50691503477147	36.0	34.0	40.0	29.0	41.0
26	35.3705971628231	36.0	34.0	40.0	29.0	41.0
27	35.15794108380436	36.0	34.0	40.0	28.0	41.0
28	35.13057513807707	36.0	34.0	40.0	28.0	41.0
29	35.046310606565086	36.0	34.0	40.0	27.0	41.0
30	35.01456406209468	36.0	34.0	40.0	27.0	41.0
31	34.894358246011244	36.0	34.0	40.0	27.0	41.0
32	34.64289249717485	36.0	33.0	40.0	25.0	41.0
33	34.434532038750035	36.0	33.0	40.0	25.0	41.0
34	34.324587706891975	36.0	33.0	40.0	24.0	41.0
35	34.12993357789813	35.0	33.0	40.0	24.0	41.0
36	33.84392744158125	35.0	33.0	40.0	23.0	41.0
37	33.90085634120864	35.0	33.0	40.0	23.0	41.0
38	33.88520098077705	35.0	33.0	40.0	23.0	41.0
39	33.81946814611471	35.0	33.0	40.0	23.0	41.0
40	33.7090999174072	35.0	33.0	40.0	23.0	41.0
41	33.7232008237414	35.0	33.0	40.0	23.0	41.0
42	33.46565789486762	35.0	32.0	40.0	22.0	41.0
43	33.50334048607501	35.0	32.0	40.0	22.0	41.0
44	33.32438743675091	35.0	32.0	40.0	21.0	41.0
45	33.15275244722161	35.0	32.0	39.0	20.0	41.0
46	33.21299549466725	35.0	32.0	39.0	22.0	41.0
47	33.06465048139376	35.0	32.0	39.0	20.0	41.0
48	33.12486520278967	35.0	32.0	39.0	21.0	41.0
49	33.04478398156918	35.0	32.0	39.0	20.0	41.0
50	32.52931398283143	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	5.0
14	19.0
15	69.0
16	228.0
17	633.0
18	1492.0
19	2879.0
20	4770.0
21	7712.0
22	11536.0
23	16412.0
24	23128.0
25	30875.0
26	38645.0
27	45675.0
28	52113.0
29	60187.0
30	70972.0
31	84936.0
32	101748.0
33	127844.0
34	197681.0
35	261836.0
36	155522.0
37	180758.0
38	231466.0
39	303139.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.89896744094516	24.658074762881146	29.45079268213898	12.992165114034712
2	29.564345355173877	27.916017735088822	28.983313471968643	13.536323437768663
3	28.496850838997716	26.716931324734805	31.41130318712785	13.374914649139633
4	25.2289192071489	29.89874182644381	30.068499345519168	14.803839620888127
5	22.810073339621383	33.25264550396018	30.043403459356092	13.89387769706234
6	20.838530583685586	42.963610468115306	25.513869328453964	10.683989619745144
7	87.47645707708959	4.675438134416548	6.322374299427218	1.5257304890666417
8	87.57554855631567	3.8979129167780657	5.750734737974101	2.7758037889321674
9	82.35714477394322	6.058296004237975	8.530712892129433	3.0538463296893776
10	48.19299680661061	26.284188796600077	13.631588415540168	11.891225981249148
11	40.95295788562438	23.543022300055362	21.967199428310742	13.536820386009515
12	38.87675783016496	21.241406522545052	24.009408224095825	15.872427423194166
13	22.0447233538838	37.25163769292773	25.25948152396135	15.444157429227115
14	16.14694163144132	39.54117762818532	27.04203486390079	17.26984587647258
15	14.623745578403028	24.884732855534164	43.79932832475767	16.692193241305144
16	16.028667950118322	19.955950507930797	44.41390421422047	19.601477327730407
17	16.165279021528793	21.755648562179655	29.678394976449624	32.40067743984193
18	19.711700447551586	24.09413789916125	34.10660136104185	22.08756029224532
19	27.360131432870745	25.6610653973946	25.994666751479166	20.984136418255492
20	31.130527431045945	23.626161740750053	25.355243449973713	19.888067378230286
21	22.323362232530034	30.9026269677908	27.01430515206119	19.759705647617977
22	22.58898106726592	26.576742225990195	23.0036346794336	27.830642027310287
23	19.79434294000543	32.11652243572223	23.12111324357123	24.968021380701114
24	19.507901973977802	24.0716758386747	37.766326985979106	18.6540952013684
25	18.63908736449464	25.476796989686335	34.16310437602682	21.721011269792207
26	17.47528427924118	34.51549037361563	27.108824707471417	20.900400639671776
27	18.675314891252818	35.79925676421098	25.862776688356803	19.662651656179403
28	17.026291543630563	30.74946751995993	34.298622161307414	17.925618775102098
29	17.244004567948227	25.359865068613647	33.91358666429456	23.48254369914356
30	19.510635189302494	31.969177282309335	28.515436703205616	20.004750825182555
31	26.684828468375706	28.898335322782792	24.43847333524824	19.978362873593262
32	29.29693750677092	26.650240870812343	25.738688712615826	18.31413290980091
33	28.610801070625293	27.197182104695067	23.560266404012957	20.631750420666688
34	20.029946100993797	27.004614661364563	27.082685230002557	25.882754007639086
35	20.361758441411293	26.060015445151326	29.559773431358032	24.01845268207935
36	29.28530891793496	25.63542286816659	24.780125250834626	20.299142963063822
37	20.667729473304437	32.74128576412252	27.481287413990685	19.109697348582355
38	19.60167610702675	31.986818944859618	25.440072514687305	22.97143243342633
39	20.651379876180375	31.56888547430231	26.226145242068455	21.553589407448857
40	25.91609923460032	25.012448553433366	26.44475277321966	22.626699438746655
41	17.6752562513604	24.140602559681	28.974318708809204	29.2098224801494
42	21.042378752083454	24.91454974998534	25.66429556096014	28.378775936971063
43	20.912029228507734	24.939297772379813	27.65730648090079	26.491366518211663
44	19.735156404519845	27.84366207122063	29.877919695152073	22.54326182910745
45	18.149543652430424	33.59270718517583	24.93850265519445	23.31924650719929
46	20.90035094484769	31.840567077576605	25.934188150567365	21.324893827008342
47	20.7969360159262	26.37537879879659	27.69497515755744	25.132710027719774
48	21.669875295808442	24.044244295779617	31.397289246735795	22.888591161676146
49	20.5928393734079	24.5541628857188	32.751721677180434	22.10127606369286
50	19.806716951202663	30.929660952093197	27.78889837507864	21.474723721625498
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2465.0
1	3325.5
2	4186.0
3	8688.0
4	13190.0
5	10646.0
6	8102.0
7	8817.0
8	9532.0
9	10403.0
10	11274.0
11	11829.0
12	12384.0
13	12482.5
14	12581.0
15	12255.5
16	11930.0
17	11857.5
18	11785.0
19	11762.0
20	11739.0
21	11655.0
22	11571.0
23	11940.0
24	12309.0
25	12663.5
26	13018.0
27	16397.5
28	19777.0
29	25394.0
30	31011.0
31	35153.0
32	39295.0
33	42374.0
34	45453.0
35	51669.5
36	57886.0
37	60957.0
38	64028.0
39	76166.0
40	88304.0
41	117623.5
42	146943.0
43	163378.0
44	179813.0
45	190602.5
46	201392.0
47	191668.0
48	181944.0
49	171642.5
50	161341.0
51	142159.5
52	122978.0
53	105866.5
54	88755.0
55	81743.0
56	74731.0
57	74260.0
58	73789.0
59	73993.5
60	74198.0
61	69414.0
62	64630.0
63	55930.0
64	47230.0
65	39458.5
66	31687.0
67	27257.5
68	22828.0
69	19647.0
70	16466.0
71	14350.0
72	12234.0
73	10434.0
74	8634.0
75	6841.0
76	5048.0
77	3871.0
78	2694.0
79	2156.5
80	1619.0
81	1297.0
82	975.0
83	664.0
84	353.0
85	232.0
86	111.0
87	76.5
88	42.0
89	31.5
90	21.0
91	12.5
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2012282.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.040331189648374
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.4765801306957	17.957708102804574
2	7.050424132480633	3.1078736581472493
3	2.343662607644125	1.5496530020781434
4	1.2582172082525824	1.1092609591360678
5	0.7826505408082252	0.8624938562585345
6	0.5542227590955119	0.7329151897983474
7	0.4489727227266749	0.6926855252809858
8	0.3459091158737761	0.6099161180301185
9	0.2990356756439008	0.5931760795840657
>10	3.3615614346443654	17.301595093290935
>50	1.1552937099831393	18.420055496527922
>100	0.9136409502251112	35.00992739344861
>500	0.007771776871988446	1.0397456946773962
>1k	0.0018286533816443404	0.6896539787982787
>5k	2.2858167270554256E-4	0.3233398521387503
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6418	0.31894138097940544	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3877	0.19266683297867793	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09610978978095515	0.0
2	0.0	0.0	0.0	0.3164566397751409	0.0
3	0.0	0.0	0.0	0.4548070300285944	0.0
4	0.0	0.0	0.0	0.7585417948379004	0.0
5	0.0	0.0	0.0	1.1609207854565116	0.0
6	0.0	0.0	0.0	1.9278610055648264	0.0
7	0.0	0.0	0.0	2.2775137878289424	0.0
8	0.0	0.0	0.0	3.2622664219030932	0.0
9	0.0	0.0	0.0	3.738044667695681	0.0
10	0.0	0.0	0.0	4.514426904380201	0.0
11	0.0	0.0	0.0	5.151713328449989	0.0
12	0.0	0.0	0.0	5.675894332901651	0.0
13	0.0	0.0	0.0	5.962732857521957	0.0
14	0.0	0.0	0.0	6.087715340096468	0.0
15	0.0	0.0	0.0	6.204746650817331	0.0
16	0.0	0.0	0.0	6.428969697090169	0.0
17	0.0	0.0	0.0	6.660597272151716	0.0
18	0.0	0.0	0.0	7.005827215072241	0.0
19	0.0	0.0	0.0	7.144426079446121	0.0
20	0.0	0.0	0.0	7.284963041959328	0.0
21	0.0	0.0	0.0	7.466249760222474	0.0
22	0.0	0.0	0.0	7.648778849087752	0.0
23	0.0	0.0	0.0	7.853273050198729	0.0
24	0.0	0.0	0.0	8.01920406781952	0.0
25	0.0	0.0	0.0	8.153280703201638	0.0
26	0.0	0.0	0.0	8.284971987027664	0.0
27	0.0	0.0	0.0	8.427049489087514	0.0
28	0.0	0.0	0.0	8.552528919902876	0.0
29	0.0	0.0	0.0	8.697289942463332	0.0
30	0.0	0.0	0.0	8.885533936098419	0.0
31	0.0	0.0	0.0	9.041525988902151	0.0
32	0.0	0.0	0.0	9.192946117890036	0.0
33	0.0	0.0	0.0	9.331495287439832	0.0
34	0.0	0.0	0.0	9.47814471331553	0.0
35	0.0	0.0	0.0	9.672898728905789	0.0
36	0.0	0.0	0.0	9.832270029747322	0.0
37	0.0	0.0	0.0	9.991889804709281	0.0
38	0.0	0.0	0.0	10.148428500577952	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATCCG	20	7.857892E-4	44.000004	1
CCTCGCA	20	7.857892E-4	44.000004	14
TAGCCGT	20	7.857892E-4	44.000004	44
CGCACGA	20	7.857892E-4	44.000004	40
CATACGT	30	2.5286681E-6	44.0	37
CGTAAAT	45	4.802132E-10	44.0	16
TAATGCG	25	4.4440363E-5	44.0	1
GTTATCG	25	4.4440363E-5	44.0	1
TATTGCG	160	0.0	42.625004	1
TATAGCG	90	0.0	41.555557	1
CGTACAT	70	0.0	40.857143	35
CGGTCTA	140	0.0	40.857143	31
TATCACG	65	0.0	40.615387	1
CCCGTAA	65	0.0	40.615387	33
CGTTTTT	5595	0.0	40.2252	1
CGGTATG	55	7.8216544E-11	40.000004	6
CGGAATA	50	1.3496901E-9	39.6	29
CTAAACG	50	1.3496901E-9	39.6	1
TCGATTG	90	0.0	39.11111	1
TTACGTC	45	2.352499E-8	39.11111	32
>>END_MODULE