FastQCFastQC Report
Thu 26 May 2016
SRR1546651_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546651_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2336499
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102040.43672177903778264No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA36930.15805699039460322No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26680.1141879367378287No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGA207.858151E-444.00000423
CTATTCG207.858151E-444.00000414
CGTCGTT207.858151E-444.00000422
GCGTACA207.858151E-444.00000437
TGCGTAC207.858151E-444.00000436
CGGTCTA1200.044.031
GTCGGAC254.4442568E-544.09
CGTTTTT106100.041.5325161
TAGTGCG750.041.0666661
CGACGGT1300.040.61538728
CTATGCG2250.040.088891
ATCGACG557.8216544E-1140.0000041
TCGATAG1000.039.6000021
TACGCGC501.3496901E-939.60000234
TAACGGG7300.039.178083
CTAGACG800.038.5000041
CTATCGA404.1269777E-738.50000443
GCACGAC404.1269777E-738.50000434
TCGTCGG404.1269777E-738.5000042
CGCTTTA404.1269777E-738.50000412