##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546651_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2336499
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.965711947661866	31.0	31.0	33.0	30.0	34.0
2	31.347454032721608	31.0	31.0	34.0	30.0	34.0
3	31.49639481977095	31.0	31.0	34.0	30.0	34.0
4	35.26475466071246	37.0	35.0	37.0	33.0	37.0
5	35.052125423550365	37.0	35.0	37.0	32.0	37.0
6	35.127789911316036	37.0	35.0	37.0	32.0	37.0
7	35.6753095122232	37.0	35.0	37.0	35.0	37.0
8	35.71389373588433	37.0	35.0	37.0	35.0	37.0
9	37.48166038162225	39.0	37.0	39.0	35.0	39.0
10	36.75251990264066	39.0	37.0	39.0	32.0	39.0
11	36.31846365010214	38.0	35.0	39.0	32.0	39.0
12	35.647154995572436	37.0	35.0	39.0	30.0	39.0
13	35.374863417446356	37.0	35.0	39.0	30.0	39.0
14	36.310257355128336	38.0	35.0	40.0	30.0	41.0
15	36.57850356452111	38.0	35.0	40.0	31.0	41.0
16	36.67862173277198	38.0	35.0	40.0	31.0	41.0
17	36.58913571116444	38.0	35.0	40.0	31.0	41.0
18	36.513835015551045	38.0	35.0	40.0	31.0	41.0
19	36.4337245596938	38.0	35.0	40.0	31.0	41.0
20	36.25966328254367	38.0	34.0	40.0	30.0	41.0
21	36.132900977060125	38.0	34.0	40.0	30.0	41.0
22	36.04252088273952	37.0	34.0	40.0	30.0	41.0
23	35.89060384789379	37.0	34.0	40.0	30.0	41.0
24	35.85193274210689	37.0	34.0	40.0	30.0	41.0
25	35.69201185192033	37.0	34.0	40.0	30.0	41.0
26	35.57710831461944	37.0	34.0	40.0	29.0	41.0
27	35.398149539118144	37.0	34.0	40.0	29.0	41.0
28	35.396494926811435	36.0	34.0	40.0	29.0	41.0
29	35.303022171205725	36.0	34.0	40.0	29.0	41.0
30	35.23187384201748	36.0	34.0	40.0	28.0	41.0
31	35.080866287552446	36.0	34.0	40.0	27.0	41.0
32	34.80350858271285	36.0	34.0	40.0	25.0	41.0
33	34.54776398363534	36.0	33.0	40.0	24.0	41.0
34	34.44434557857718	36.0	33.0	40.0	24.0	41.0
35	34.18179635428905	36.0	33.0	40.0	23.0	41.0
36	33.91725440498798	36.0	33.0	40.0	22.0	41.0
37	33.926909448709374	36.0	33.0	40.0	23.0	41.0
38	33.888612834843926	36.0	33.0	40.0	23.0	41.0
39	33.8107433386447	36.0	33.0	40.0	21.0	41.0
40	33.73149357222066	36.0	33.0	40.0	21.0	41.0
41	33.753352772673985	36.0	33.0	40.0	21.0	41.0
42	33.5079582743241	36.0	33.0	40.0	19.0	41.0
43	33.54360733730252	36.0	33.0	40.0	20.0	41.0
44	33.340861262940834	35.0	32.0	40.0	18.0	41.0
45	33.178596267321325	35.0	32.0	40.0	18.0	41.0
46	33.2577313322197	35.0	32.0	40.0	18.0	41.0
47	33.13689113498444	35.0	32.0	40.0	18.0	41.0
48	33.17801419987768	35.0	32.0	40.0	18.0	41.0
49	33.11896645365566	36.0	32.0	40.0	18.0	41.0
50	32.610959388384074	35.0	31.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	4.0
13	6.0
14	21.0
15	89.0
16	294.0
17	864.0
18	1976.0
19	3863.0
20	6501.0
21	10250.0
22	15149.0
23	20829.0
24	29130.0
25	37959.0
26	47233.0
27	53888.0
28	59022.0
29	66302.0
30	76570.0
31	91290.0
32	110366.0
33	136997.0
34	208371.0
35	282696.0
36	173482.0
37	209325.0
38	285881.0
39	408140.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.981652463793054	25.90705153308433	27.38015295534045	15.731143047782172
2	28.258518407240917	27.893955871583938	30.305341453174172	13.54218426800097
3	26.323358152517933	27.30409899597646	33.89370164506811	12.478841206437496
4	23.71034612041349	30.12139102135289	32.566373878182695	13.601888980050921
5	22.31560980766523	34.1335048720329	31.212168291105623	12.338717029196246
6	20.407070578673476	42.407679181544694	27.353317934225522	9.831932305556304
7	87.40709925405488	4.583181931599372	6.890608555792234	1.1191102585535024
8	88.06954336381055	3.784080369818262	6.260049758206616	1.886326508164566
9	82.97889277932497	6.61562448774855	8.175222844092806	2.2302598888336784
10	43.05356004860263	34.729182422076796	12.474732495070615	9.742525034249962
11	34.13667200371154	24.29994620156054	28.194362591210183	13.369019203517743
12	32.88039070421173	23.933414908373596	28.40527644137661	14.78091794603807
13	20.005401243484375	37.44730898665054	28.522417514409383	14.024872255455707
14	15.787766226306966	39.233057664480064	29.40861519735296	15.57056091186001
15	14.188407527672814	26.400867280491024	44.92631924944115	14.484405942395009
16	16.15575268810301	23.353059427802023	44.22120446017738	16.26998342391758
17	16.454575841889937	23.028813622432537	30.960295724500632	29.556314811176893
18	19.04190842795139	24.955970449805456	36.88304595893258	19.11907516331058
19	25.137096142562015	26.001594693599266	29.829201724460404	19.03210743937832
20	26.703199958570494	26.04863087893468	29.262156756754443	17.986012405740386
21	20.544412815926734	30.212467456652025	31.203736872988173	18.039382854433065
22	21.019824960335956	26.67782010606467	28.338124689974187	23.964230243625185
23	16.87353600408132	32.231599499935584	28.698236121650382	22.196628374332708
24	17.220636516429067	25.648630707738374	40.17057144043289	16.960161335399672
25	16.071481306005268	27.26523743429807	37.960384318589476	18.702896941107188
26	16.238269308054488	34.945403357758764	29.778313622218544	19.038013711968205
27	16.162557741304404	35.807248366038245	30.120107049050738	17.91008684360661
28	15.082266245352555	31.645294947697387	37.22796371836667	16.04447508858339
29	15.5031951650739	26.92340120838913	36.9860633366417	20.587340289895266
30	17.601976290167467	32.827705040746864	32.06125917451709	17.509059494568582
31	24.39680051221935	29.392693940806307	28.47033103801885	17.74017450895549
32	25.857062211453975	27.760850742927772	29.670973537758844	16.71111350785941
33	25.14458598099122	29.542490709390417	27.096566272872362	18.216357036746004
34	18.38729654923884	29.51420051966639	29.703886027770608	22.39461690332416
35	18.141287456146994	27.514028467377898	32.723104097198416	21.621579979276685
36	27.388584373457896	26.260785902326518	28.27195731733675	18.07867240687884
37	19.090699375433072	32.98139652531415	30.118651880441632	17.809252218811135
38	19.28196844937661	33.11852476718372	26.87431066737028	20.725196116069384
39	19.780834487838426	32.66892046604771	28.48047441920583	19.069770626908035
40	24.04499210143039	26.949251850739074	27.726311887999955	21.279444159830586
41	17.068400200470876	26.061812994570083	30.124472554878047	26.745314250080998
42	19.826971892562334	26.822908976207565	27.89083153898204	25.45928759224806
43	19.981818952201564	26.330633995563446	29.83955910103107	23.847987951203915
44	18.46228053168437	29.852741216666473	31.471231102602655	20.2137471490465
45	17.16414173513449	35.59984404016436	26.443623558152602	20.792390666548542
46	19.928876494276267	33.191711188406245	28.450557864565745	18.428854452751743
47	19.497247805370343	28.55490201365376	29.052526878890166	22.89532330208573
48	19.75639621502085	26.402365248176867	33.820943214612974	20.02029532218931
49	20.811949844617953	25.077905019432922	33.62226990039371	20.48787523555542
50	18.96114657014619	32.915571545290625	28.753104538028907	19.37017734653428
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3786.0
1	4133.5
2	4481.0
3	12406.5
4	20332.0
5	16365.0
6	12398.0
7	13672.5
8	14947.0
9	16424.0
10	17901.0
11	18916.0
12	19931.0
13	20115.5
14	20300.0
15	20211.5
16	20123.0
17	19987.5
18	19852.0
19	18896.0
20	17940.0
21	18553.0
22	19166.0
23	20821.5
24	22477.0
25	25288.0
26	28099.0
27	32862.5
28	37626.0
29	42613.0
30	47600.0
31	57185.0
32	66770.0
33	71127.0
34	75484.0
35	86657.0
36	97830.0
37	102234.0
38	106638.0
39	122597.5
40	138557.0
41	169858.5
42	201160.0
43	223331.0
44	245502.0
45	235110.5
46	224719.0
47	206801.0
48	188883.0
49	177169.0
50	165455.0
51	144312.0
52	123169.0
53	107130.5
54	91092.0
55	82042.5
56	72993.0
57	65413.0
58	57833.0
59	52798.5
60	47764.0
61	42323.5
62	36883.0
63	31673.0
64	26463.0
65	21417.0
66	16371.0
67	12911.5
68	9452.0
69	8219.5
70	6987.0
71	5212.5
72	3438.0
73	3039.5
74	2641.0
75	2138.0
76	1635.0
77	1252.0
78	869.0
79	762.0
80	655.0
81	410.5
82	166.0
83	121.5
84	77.0
85	58.0
86	39.0
87	24.5
88	10.0
89	6.5
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2336499.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.816739471283167
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.60035469092865	18.02070418527573
2	6.360795378015689	2.775436311846208
3	2.177239565095123	1.425008050747505
4	1.1137763888241892	0.9719587721697567
5	0.7088112906897199	0.773197563164079
6	0.5366061484504894	0.7024197923659815
7	0.41842919634290515	0.6390132534654086
8	0.3415835858972981	0.5961792080950421
9	0.2941980742896985	0.5776598465758407
>10	3.369164318532157	17.116310215607623
>50	1.0774893971889985	17.004592128729286
>100	0.9932023868325406	37.540133249621874
>500	0.0071567819250331925	0.9979364203274625
>1k	9.939974895879433E-4	0.4168875167190043
>5k	0.0	0.0
>10k+	1.987994979175887E-4	0.44256348528929673
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10204	0.43672177903778264	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3693	0.15805699039460322	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2668	0.1141879367378287	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04425424534741937	0.0
2	0.0	0.0	0.0	0.15048155381192116	0.0
3	0.0	0.0	0.0	0.21818969321193804	0.0
4	0.0	0.0	0.0	0.3946930856807557	0.0
5	0.0	0.0	0.0	0.5839078039408534	0.0
6	0.0	0.0	0.0	1.0276486315637199	0.0
7	0.0	0.0	0.0	1.2142954052195185	0.0
8	0.0	0.0	0.0	1.7310514577579532	0.0
9	0.0	0.0	0.0	1.9994444679839367	0.0
10	0.0	0.0	0.0	2.427520833520579	0.0
11	0.0	0.0	0.0	2.7551049668756544	0.0
12	0.0	0.0	0.0	3.0475938573053103	0.0
13	0.0	0.0	0.0	3.206549628311418	0.0
14	0.0	0.0	0.0	3.2859419156609952	0.0
15	0.0	0.0	0.0	3.357501971967461	0.0
16	0.0	0.0	0.0	3.4724174930098406	0.0
17	0.0	0.0	0.0	3.604238649363856	0.0
18	0.0	0.0	0.0	3.8125417558492427	0.0
19	0.0	0.0	0.0	3.9058009440620345	0.0
20	0.0	0.0	0.0	4.003853628869518	0.0
21	0.0	0.0	0.0	4.117570775763225	0.0
22	0.0	0.0	0.0	4.237664985090942	0.0
23	0.0	0.0	0.0	4.369486141444956	0.0
24	0.0	0.0	0.0	4.477810604669636	0.0
25	0.0	0.0	0.0	4.571112591959166	0.0
26	0.0	0.0	0.0	4.658208713121641	0.0
27	0.0	0.0	0.0	4.747787180734937	0.0
28	0.0	0.0	0.0	4.836381269583253	0.0
29	0.0	0.0	0.0	4.933663571009446	0.0
30	0.0	0.0	0.0	5.074986122399368	0.0
31	5.563879975981159E-4	0.0	0.0	5.1826258003962336	0.0
32	5.563879975981159E-4	0.0	0.0	5.286327963333175	0.0
33	5.563879975981159E-4	0.0	0.0	5.386991391821696	0.0
34	5.563879975981159E-4	0.0	0.0	5.494331476281394	0.0
35	5.563879975981159E-4	0.0	0.0	5.62662342247953	0.0
36	5.991870743364325E-4	0.0	0.0	5.747231220728106	0.0
37	5.991870743364325E-4	0.0	0.0	5.868095813437113	0.0
38	5.991870743364325E-4	0.0	0.0	5.994866678736006	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCGA	20	7.858151E-4	44.000004	23
CTATTCG	20	7.858151E-4	44.000004	14
CGTCGTT	20	7.858151E-4	44.000004	22
GCGTACA	20	7.858151E-4	44.000004	37
TGCGTAC	20	7.858151E-4	44.000004	36
CGGTCTA	120	0.0	44.0	31
GTCGGAC	25	4.4442568E-5	44.0	9
CGTTTTT	10610	0.0	41.532516	1
TAGTGCG	75	0.0	41.066666	1
CGACGGT	130	0.0	40.615387	28
CTATGCG	225	0.0	40.08889	1
ATCGACG	55	7.8216544E-11	40.000004	1
TCGATAG	100	0.0	39.600002	1
TACGCGC	50	1.3496901E-9	39.600002	34
TAACGGG	730	0.0	39.17808	3
CTAGACG	80	0.0	38.500004	1
CTATCGA	40	4.1269777E-7	38.500004	43
GCACGAC	40	4.1269777E-7	38.500004	34
TCGTCGG	40	4.1269777E-7	38.500004	2
CGCTTTA	40	4.1269777E-7	38.500004	12
>>END_MODULE