FastQCFastQC Report
Thu 26 May 2016
SRR1546650_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546650_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1813301
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67780.3737934297725529No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA20940.11548000028676982No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGACG207.857688E-444.01
TAGTACG351.4466968E-744.01
CGTTTTT72700.041.579091
TATAGCG1150.040.173911
ATCGTAG452.3523171E-839.111111
ACGGGAT10150.038.7980275
AGGGCGA18550.038.7816736
CGCATCG404.1263047E-738.521
CGACAGG1900.038.2105262
CGTTGAT3200.037.812525
TGCGAGG6150.037.5609742
CGAGGGA12700.037.417324
TAATCGT3300.037.33333221
TTAATCG3250.037.2307720
TAGCGCG651.0913936E-1137.230771
AGGGATC27750.037.10276
AGGGATT47450.037.045316
GCGATGA4350.036.919549
TAGGGAT41550.036.7990385
GGGCGAT36650.036.7967227
Produced by FastQC (version 0.11.5)