##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546650_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1813301
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.944769235774977	31.0	31.0	33.0	30.0	34.0
2	31.324149162218518	31.0	31.0	34.0	30.0	34.0
3	31.465035314048798	31.0	31.0	34.0	30.0	34.0
4	35.24671910510169	37.0	35.0	37.0	33.0	37.0
5	35.036892385764965	37.0	35.0	37.0	32.0	37.0
6	35.11478734087722	37.0	35.0	37.0	32.0	37.0
7	35.68022959232913	37.0	35.0	37.0	35.0	37.0
8	35.72656001402966	37.0	35.0	37.0	35.0	37.0
9	37.49400954392018	39.0	37.0	39.0	35.0	39.0
10	36.70868873948672	39.0	37.0	39.0	32.0	39.0
11	36.28794833290226	38.0	35.0	39.0	31.0	39.0
12	35.85000945788923	37.0	35.0	39.0	31.0	39.0
13	35.6965760235063	37.0	35.0	39.0	30.0	39.0
14	36.63018329554773	38.0	35.0	40.0	31.0	41.0
15	36.81510571052462	38.0	35.0	40.0	31.0	41.0
16	36.866714902820874	38.0	35.0	40.0	31.0	41.0
17	36.77346066648615	38.0	35.0	40.0	31.0	41.0
18	36.689936750710444	38.0	35.0	40.0	31.0	41.0
19	36.66072814165988	38.0	35.0	40.0	31.0	41.0
20	36.52662961085887	38.0	35.0	40.0	31.0	41.0
21	36.428219032582014	38.0	35.0	40.0	30.0	41.0
22	36.36062297434348	38.0	35.0	40.0	30.0	41.0
23	36.17712834217816	38.0	34.0	40.0	30.0	41.0
24	36.13737818486837	38.0	34.0	40.0	30.0	41.0
25	35.984863516867854	38.0	34.0	40.0	30.0	41.0
26	35.86976348659158	38.0	34.0	40.0	30.0	41.0
27	35.694422492459886	38.0	34.0	40.0	29.0	41.0
28	35.66099119782099	38.0	34.0	40.0	29.0	41.0
29	35.54864470928985	37.0	34.0	40.0	29.0	41.0
30	35.51184221483361	38.0	34.0	40.0	28.0	41.0
31	35.43522834874078	38.0	34.0	40.0	27.0	41.0
32	35.227406260736636	38.0	34.0	40.0	27.0	41.0
33	35.02822697390009	38.0	34.0	40.0	26.0	41.0
34	34.94788399719627	38.0	34.0	40.0	25.0	41.0
35	34.726327840772164	37.0	33.0	40.0	24.0	41.0
36	34.528759428247156	37.0	33.0	40.0	24.0	41.0
37	34.624420876622246	37.0	33.0	40.0	24.0	41.0
38	34.578705355591815	37.0	33.0	40.0	24.0	41.0
39	34.48858297657146	37.0	33.0	40.0	23.0	41.0
40	34.490573820893495	37.0	33.0	40.0	23.0	41.0
41	34.51342330920239	37.0	33.0	40.0	23.0	41.0
42	34.27718729543523	37.0	33.0	40.0	23.0	41.0
43	34.33093347436526	37.0	33.0	40.0	23.0	41.0
44	34.14957417439245	37.0	33.0	40.0	23.0	41.0
45	33.978658810644234	37.0	33.0	40.0	23.0	41.0
46	34.05601000606077	37.0	33.0	40.0	23.0	41.0
47	33.89890260910902	37.0	33.0	40.0	23.0	41.0
48	33.963112577558825	37.0	33.0	40.0	23.0	41.0
49	33.87522920905023	36.0	33.0	40.0	23.0	41.0
50	33.3955041110108	36.0	32.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	6.0
14	12.0
15	41.0
16	186.0
17	489.0
18	1059.0
19	2251.0
20	3808.0
21	5852.0
22	8856.0
23	12330.0
24	18191.0
25	24462.0
26	31545.0
27	37805.0
28	43015.0
29	48993.0
30	58042.0
31	68838.0
32	83115.0
33	103434.0
34	147382.0
35	187562.0
36	149025.0
37	182134.0
38	249745.0
39	345121.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.742441547211413	25.315819050449978	29.89928313059994	14.042456271738669
2	30.748838720102178	26.13653221390161	29.962813675170313	13.151815390825902
3	27.102725912576016	25.546227570601904	33.55835572803412	13.792690788787962
4	24.180376010381067	28.834705324708914	32.56365049156207	14.421268173347942
5	21.417403950033666	33.42197461976804	31.29717570331677	13.863445726881526
6	20.89608950747835	42.01139248254978	27.61345193103627	9.479066078935599
7	89.64871248623368	3.1927959009563223	6.087957818365512	1.0705337944444966
8	90.41455334773433	2.5986308947052916	5.538186986054715	1.4486287715056685
9	84.76640116560901	5.681516747633184	7.341748556913606	2.21033352984419
10	39.27224437641627	38.09902492746654	12.967510633921231	9.661220062195962
11	28.768748266283424	26.023975059849413	30.627733619514906	14.579543054352257
12	29.295191476759786	24.62415230565692	29.925699042795433	16.15495717478786
13	21.38839607985657	32.07410132129194	30.93860313318087	15.598899465670618
14	18.193063368960807	33.890181497721564	30.846340458644207	17.070414674673426
15	16.276282867543777	26.581246025894213	39.90810130254161	17.234369804020403
16	19.11795118405604	24.906124245230107	38.61261864411921	17.363305926594645
17	19.606231949356452	23.79345734657401	31.53524980132918	25.06506090274036
18	20.501174377557835	25.715752652207218	34.46030195759005	19.322771012644896
19	24.72794092100539	26.29789538526698	29.967446110711897	19.006717583015725
20	25.942135365281327	26.901159818474706	28.89525787500255	18.261446941241417
21	21.989675183546474	29.066161657661908	30.719941146009404	18.224222012782214
22	22.05155128685199	26.091145375202462	29.47205124797262	22.38525208997293
23	19.33959116550424	29.881856349276813	29.700970770986174	21.07758171423277
24	18.893994984837047	26.74988873882494	36.51263634664074	17.843479929697274
25	18.89658694281865	27.825606449232644	33.81975744788097	19.458049160067745
26	18.477903006726407	32.29375597322232	29.65244049388381	19.57590052616747
27	18.116131850145123	32.14347755833146	30.465488079474945	19.27490251204847
28	17.22339534363021	29.579259041935124	34.7856202583024	18.41172535613227
29	18.18230950073926	26.28708636900327	34.66021361042651	20.870390519830963
30	19.176242664621043	30.197799482821665	31.87887725203924	18.74708060051806
31	23.833329381056977	28.216550920117513	29.266955679172955	18.68316401965256
32	24.3534857147269	28.02684165508098	28.975332832221458	18.644339797970662
33	23.758603783927764	28.029433613062587	29.15191686322348	19.06004573978617
34	20.176628149435754	27.83073521715369	29.945331745805028	22.04730488760553
35	19.456891051182346	27.70003435723027	32.00571774901133	20.837356842576053
36	24.116404281473404	28.232929888639557	28.75198326146624	18.898682568420796
37	19.982065856688987	31.796596373133863	29.52140874570742	18.69992902446974
38	19.89829598064524	31.509550813681784	28.5263726209824	20.06578058469057
39	19.948149810759492	31.68431495929247	29.21660551667925	19.150929713268784
40	22.875352740664677	27.96888106276895	28.71155974656166	20.444206450004714
41	18.432405871942937	27.573965932848438	29.36236179211284	24.631266403095793
42	20.19515789160211	27.948917471506384	28.153571855968757	23.702352780922748
43	20.399260795642864	27.54843238932753	29.29524662480195	22.75706019022766
44	18.99596371479418	30.19934362800219	30.11948926295193	20.6852033942517
45	18.727668489677114	32.82891257436024	27.263206715266797	21.180212220695847
46	20.253835408462244	31.06902825289348	28.243077128397324	20.434059210246946
47	19.54562425102065	28.807517339923155	29.435874132314492	22.210984276741698
48	20.671085495458282	27.47045305771077	31.615986535054024	20.24247491177692
49	20.3266308241158	27.59668692621909	31.883399391496503	20.193282858168608
50	19.432350172420353	31.375816811439467	29.2435177612542	19.94831525488598
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1447.0
1	1850.0
2	2253.0
3	8029.5
4	13806.0
5	10997.0
6	8188.0
7	8504.5
8	8821.0
9	9400.5
10	9980.0
11	10181.0
12	10382.0
13	10514.0
14	10646.0
15	10907.5
16	11169.0
17	11231.5
18	11294.0
19	11587.5
20	11881.0
21	12066.0
22	12251.0
23	13958.0
24	15665.0
25	18583.5
26	21502.0
27	25794.5
28	30087.0
29	35858.5
30	41630.0
31	46642.0
32	51654.0
33	60014.0
34	68374.0
35	77760.0
36	87146.0
37	92226.5
38	97307.0
39	105231.0
40	113155.0
41	127237.0
42	141319.0
43	154004.5
44	166690.0
45	164950.5
46	163211.0
47	154236.5
48	145262.0
49	134054.0
50	122846.0
51	113991.0
52	105136.0
53	91612.0
54	78088.0
55	67758.0
56	57428.0
57	53107.0
58	48786.0
59	45480.0
60	42174.0
61	38202.5
62	34231.0
63	29647.5
64	25064.0
65	20231.0
66	15398.0
67	12832.0
68	10266.0
69	8665.5
70	7065.0
71	5739.0
72	4413.0
73	3804.0
74	3195.0
75	2714.5
76	2234.0
77	1551.5
78	869.0
79	691.0
80	513.0
81	405.0
82	297.0
83	214.0
84	131.0
85	78.0
86	25.0
87	20.5
88	16.0
89	10.5
90	5.0
91	3.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1813301.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.953690469426085
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.0277172929572	18.690517880713823
2	6.293415402474486	3.0150104909278492
3	2.479638213889375	1.7818945875499999
4	1.562745627153836	1.4973410014116852
5	1.0299981101785713	1.2336127957655663
6	0.814519365492279	1.170644685741323
7	0.707297212005005	1.185966494037915
8	0.582212823263635	1.1156916604630231
9	0.5175324578943836	1.1157131073855018
>10	6.311153801829635	34.856670352596005
>50	1.2800450954985878	21.060292786529438
>100	0.39302484991135744	12.727145205060456
>500	2.3324915054546633E-4	0.05380452370109029
>1k	2.3324915054546633E-4	0.11699550636561065
>5k	2.3324915054546633E-4	0.3786989217507684
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6778	0.3737934297725529	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2094	0.11548000028676982	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.029338758430067596	0.0
2	0.0	0.0	0.0	0.11619692483487297	0.0
3	0.0	0.0	0.0	0.17564651428527311	0.0
4	0.0	0.0	0.0	0.2822476797839962	0.0
5	0.0	0.0	0.0	0.45419927524442993	0.0
6	0.0	0.0	0.0	0.7507302979483274	0.0
7	0.0	0.0	0.0	0.8999609000381072	0.0
8	0.0	0.0	0.0	1.3145638810103784	0.0
9	0.0	0.0	0.0	1.5086849894198482	0.0
10	0.0	0.0	0.0	1.7862450856200929	0.0
11	0.0	0.0	0.0	2.038933414805374	0.0
12	0.0	0.0	0.0	2.257485105892513	0.0
13	0.0	0.0	0.0	2.37318569834793	0.0
14	0.0	0.0	0.0	2.4277822600880934	0.0
15	0.0	0.0	0.0	2.4829854502920363	0.0
16	0.0	0.0	0.0	2.5833548870264784	0.0
17	0.0	0.0	0.0	2.7070519455953534	0.0
18	0.0	0.0	0.0	2.8846286413562887	0.0
19	0.0	0.0	0.0	2.9696117743275936	0.0
20	0.0	0.0	0.0	3.067499549164755	0.0
21	0.0	0.0	0.0	3.180001555174789	0.0
22	0.0	0.0	0.0	3.316051775187903	0.0
23	0.0	0.0	0.0	3.4659441537836244	0.0
24	0.0	0.0	0.0	3.580541785395806	0.0
25	0.0	0.0	0.0	3.6814075545097036	0.0
26	0.0	0.0	0.0	3.7841483570571017	0.0
27	0.0	0.0	0.0	3.8885987489115155	0.0
28	0.0	0.0	0.0	4.002644900102079	0.0
29	0.0	0.0	0.0	4.123970592857997	0.0
30	0.0	0.0	0.0	4.292116973409269	0.0
31	0.0	0.0	0.0	4.425078903061323	0.0
32	0.0	0.0	0.0	4.560301902442011	0.0
33	0.0	0.0	0.0	4.695966086159992	0.0
34	0.0	0.0	0.0	4.836648741714696	0.0
35	0.0	0.0	0.0	4.993489773622802	0.0
36	0.0	0.0	0.0	5.139025456887742	0.0
37	0.0	0.0	0.0	5.2992305193677165	0.0
38	0.0	0.0	0.0	5.4597113220585	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGACG	20	7.857688E-4	44.0	1
TAGTACG	35	1.4466968E-7	44.0	1
CGTTTTT	7270	0.0	41.57909	1
TATAGCG	115	0.0	40.17391	1
ATCGTAG	45	2.3523171E-8	39.11111	1
ACGGGAT	1015	0.0	38.798027	5
AGGGCGA	1855	0.0	38.781673	6
CGCATCG	40	4.1263047E-7	38.5	21
CGACAGG	190	0.0	38.210526	2
CGTTGAT	320	0.0	37.8125	25
TGCGAGG	615	0.0	37.560974	2
CGAGGGA	1270	0.0	37.41732	4
TAATCGT	330	0.0	37.333332	21
TTAATCG	325	0.0	37.23077	20
TAGCGCG	65	1.0913936E-11	37.23077	1
AGGGATC	2775	0.0	37.1027	6
AGGGATT	4745	0.0	37.04531	6
GCGATGA	435	0.0	36.91954	9
TAGGGAT	4155	0.0	36.799038	5
GGGCGAT	3665	0.0	36.796722	7
>>END_MODULE