FastQCFastQC Report
Thu 26 May 2016
SRR1546649_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546649_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1145978
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58780.5129243318807167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACGG800.041.252
CGTTTTT41350.041.2333761
ACATACG4200.039.80952517
GCGATAA1000.039.6000029
TACGGGA1950.039.487184
TACGGGT1450.039.4482774
TCGTTAG404.124522E-738.51
CGAAAGG2350.038.382982
CATACGA4300.038.37209318
TGGGCGA8450.038.0118336
GGGCGAT25700.037.922187
ACGAGTA357.2901876E-637.71428737
ATAGGGC5450.037.5412834
TAGGACG651.0913936E-1137.2307661
TAGGGTA5450.037.1376155
GCGATAT950.037.052639
TAAGGGA10500.036.876194
GGACTAA8950.036.8715068
GCGATGA2750.036.89
TTACGGG3350.036.7761153
Produced by FastQC (version 0.11.5)