##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546649_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1145978
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.07925544818487	31.0	31.0	33.0	30.0	34.0
2	31.458792402646473	31.0	31.0	34.0	30.0	34.0
3	31.62083565304046	31.0	31.0	34.0	30.0	34.0
4	35.408383930581564	37.0	35.0	37.0	33.0	37.0
5	35.230594304602704	37.0	35.0	37.0	33.0	37.0
6	35.292352907298394	37.0	35.0	37.0	32.0	37.0
7	35.70369937293735	37.0	35.0	37.0	35.0	37.0
8	35.73789549188553	37.0	35.0	37.0	35.0	37.0
9	37.546143119675946	39.0	37.0	39.0	35.0	39.0
10	36.84321863072415	39.0	37.0	39.0	32.0	39.0
11	36.479503969535195	38.0	35.0	39.0	32.0	39.0
12	35.849972687084744	37.0	35.0	39.0	31.0	39.0
13	35.622119272795814	37.0	35.0	39.0	30.0	39.0
14	36.510700030890646	38.0	35.0	40.0	31.0	41.0
15	36.75848052929463	38.0	35.0	40.0	31.0	41.0
16	36.84140358715438	38.0	35.0	40.0	31.0	41.0
17	36.769910940698686	38.0	35.0	40.0	31.0	41.0
18	36.7019070174122	38.0	35.0	40.0	31.0	41.0
19	36.63348772838571	38.0	35.0	40.0	31.0	41.0
20	36.48751633975521	38.0	35.0	40.0	31.0	41.0
21	36.3851662073792	38.0	35.0	40.0	31.0	41.0
22	36.30209916769781	38.0	35.0	40.0	30.0	41.0
23	36.122244929658336	38.0	34.0	40.0	30.0	41.0
24	36.05530298138358	38.0	34.0	40.0	30.0	41.0
25	35.90661251786683	37.0	34.0	40.0	30.0	41.0
26	35.77134726844669	37.0	34.0	40.0	30.0	41.0
27	35.59432641813368	37.0	34.0	40.0	29.0	41.0
28	35.54610297928931	37.0	34.0	40.0	29.0	41.0
29	35.40722073198613	37.0	34.0	40.0	29.0	41.0
30	35.38155095473037	37.0	34.0	40.0	29.0	41.0
31	35.2873353589685	37.0	34.0	40.0	28.0	41.0
32	35.11539139494825	37.0	34.0	40.0	27.0	41.0
33	34.911066355549586	37.0	34.0	40.0	26.0	41.0
34	34.75031283323066	37.0	34.0	40.0	25.0	41.0
35	34.531273724277426	37.0	33.0	40.0	24.0	41.0
36	34.300041536574	36.0	33.0	40.0	23.0	41.0
37	34.33185366560266	37.0	33.0	40.0	23.0	41.0
38	34.270647429531806	36.0	33.0	40.0	23.0	41.0
39	34.199203649633766	36.0	33.0	40.0	23.0	41.0
40	34.108863346416776	36.0	33.0	40.0	23.0	41.0
41	34.10721933579877	36.0	33.0	40.0	23.0	41.0
42	33.89751286673915	36.0	33.0	40.0	23.0	41.0
43	33.92609543987756	36.0	33.0	40.0	23.0	41.0
44	33.72538478051062	35.0	33.0	40.0	23.0	41.0
45	33.54366488710953	35.0	32.0	40.0	23.0	41.0
46	33.60189026316387	35.0	33.0	40.0	23.0	41.0
47	33.468261170807814	35.0	32.0	40.0	23.0	41.0
48	33.50118152355456	35.0	32.0	39.0	23.0	41.0
49	33.40031571286709	35.0	32.0	39.0	22.0	41.0
50	32.91947838440179	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	2.0
14	10.0
15	38.0
16	110.0
17	248.0
18	650.0
19	1203.0
20	2317.0
21	3615.0
22	5523.0
23	7949.0
24	11237.0
25	15456.0
26	20178.0
27	23930.0
28	26940.0
29	30717.0
30	36258.0
31	44272.0
32	53926.0
33	68691.0
34	104516.0
35	133461.0
36	97764.0
37	118065.0
38	150961.0
39	187937.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.67630094120481	24.005434659304107	28.36075387136577	13.957510528125322
2	31.340915794195002	25.627978896628033	29.48267767793099	13.548427631245975
3	28.989823539369862	25.765154304881943	31.837609447999878	13.407412707748318
4	24.900390757937764	28.50011082237181	31.762040807066107	14.837457612624327
5	22.18855859362047	33.262418650270774	30.46437191638932	14.084650839719437
6	21.801378385972505	41.2945100167717	27.273298440284194	9.6308131569716
7	88.55082732827331	3.8303527641891906	6.507891076443004	1.1109288310944887
8	89.1039793085033	3.0780695615448117	6.224465042086322	1.5934860878655612
9	84.29742979359115	4.968943557380682	8.253125278146701	2.4805013708814654
10	46.0048098654599	26.661070282326538	15.071668042492961	12.262451809720606
11	37.528032824364864	24.71557045597734	22.73132643035032	15.025070289307473
12	34.37884496910063	22.088992982413274	26.736551661550223	16.795610386935873
13	23.346521486450875	32.197389478681096	28.013539526936814	16.44254950793122
14	19.902476312808798	32.822532369731356	29.716539060959285	17.558452256500562
15	18.624354045191094	23.449664827771564	39.95800966510701	17.967971461930336
16	20.89804516317067	20.57465326559498	38.628926558799556	19.898375012434794
17	21.802425526493526	20.85223276537595	29.677620338261292	27.66772136986923
18	23.41153146046434	22.53926340645283	32.84103185226942	21.20817328081342
19	27.48848581735426	24.750038831460987	26.96570091223392	20.795774438950833
20	29.248990818322866	23.84208073802464	27.268760831359764	19.640167612292732
21	25.219506831719286	26.392129691843998	28.947763395108804	19.440600081327915
22	25.02770559295205	23.864506997516532	26.77337610320617	24.33441130632525
23	22.154264741556993	27.542151769056648	26.738296895751922	23.565286593634433
24	21.56044880442731	24.57952944995454	35.265598466986276	18.594423278631876
25	21.482349573901068	25.187394522407935	32.03194127635958	21.298314627331415
26	20.31251908849908	28.996106382496002	27.90376429564965	22.787610233355267
27	20.26068563270848	30.197176560108485	28.711022375647698	20.831115431535334
28	19.828042074106133	28.084221512105817	32.54320763574868	19.544528778039368
29	20.270022635687596	24.264078367996593	32.60280738373686	22.863091612578952
30	21.46638068095548	28.679695421727118	30.633921419084835	19.220002478232566
31	26.234185996589815	27.58901130737239	26.840044049711253	19.336758646326544
32	27.68098514980218	25.40607236788141	27.51911467759416	19.393827804722257
33	27.584473698447965	26.810898638542803	25.723268684041056	19.881358978968183
34	22.177650879859822	26.627910832494166	28.110574548551543	23.08386373909447
35	22.116654944510277	26.69361890018831	29.43101874556056	21.75870740974085
36	28.999684112609494	25.992994629914364	25.347607022124336	19.659714235351814
37	22.519280474843324	30.43985137585538	27.50969041290496	19.531177736396334
38	23.150008115339038	29.567234275003536	26.033833110234227	21.2489244994232
39	22.692756754492667	30.011919949597637	25.85887338151343	21.436449914396263
40	25.232334303101805	25.669340947208415	26.625642028031955	22.472682721657833
41	20.82125485829571	24.731888395763267	28.588594196398187	25.858262549542836
42	22.123112311056584	25.48556778576901	26.696062228070694	25.69525767510371
43	22.12389766644735	25.4991806125423	28.16642204300606	24.21049967800429
44	21.573014490679576	27.401573154109414	28.46668958740918	22.558722767801825
45	19.92036496337626	31.59685438987485	25.93409297560686	22.54868767114203
46	22.695287344085138	29.368626622849654	27.111253444656008	20.8248325884092
47	21.645528971760367	28.086752101698288	28.021044034004145	22.246674892537204
48	22.61727537526898	26.7761684779289	29.541579332238488	21.06497681456363
49	23.117459497477263	26.12877385080691	29.655194078769398	21.098572572946424
50	22.195365007007116	29.09418854463175	27.82557780341333	20.88486864494781
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1349.0
1	1828.0
2	2307.0
3	6401.0
4	10495.0
5	7866.0
6	5237.0
7	5587.0
8	5937.0
9	6300.5
10	6664.0
11	6793.0
12	6922.0
13	6972.5
14	7023.0
15	6583.5
16	6144.0
17	6311.0
18	6478.0
19	6360.0
20	6242.0
21	6064.5
22	5887.0
23	6715.0
24	7543.0
25	8265.0
26	8987.0
27	10103.0
28	11219.0
29	13341.5
30	15464.0
31	19370.0
32	23276.0
33	26064.5
34	28853.0
35	32464.0
36	36075.0
37	38439.5
38	40804.0
39	46644.0
40	52484.0
41	63583.0
42	74682.0
43	82083.0
44	89484.0
45	88650.0
46	87816.0
47	84698.0
48	81580.0
49	79780.0
50	77980.0
51	70931.5
52	63883.0
53	60158.0
54	56433.0
55	54721.0
56	53009.0
57	53382.0
58	53755.0
59	51544.5
60	49334.0
61	46302.0
62	43270.0
63	39638.5
64	36007.0
65	31268.5
66	26530.0
67	22556.0
68	18582.0
69	15851.0
70	13120.0
71	11485.5
72	9851.0
73	8137.5
74	6424.0
75	5251.0
76	4078.0
77	3241.5
78	2405.0
79	1857.5
80	1310.0
81	965.0
82	620.0
83	453.0
84	286.0
85	189.5
86	93.0
87	63.5
88	34.0
89	22.5
90	11.0
91	9.0
92	7.0
93	4.5
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1145978.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.767651498648746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.15416799101726	18.100057249800496
2	5.973374499619397	2.8394616675573836
3	2.7568041108198886	1.9656827806802804
4	1.7148321122123322	1.6303012808701771
5	1.2366140884398504	1.4695706346178778
6	0.9938479141969653	1.4172858520375458
7	0.8394560123738206	1.3966328565382433
8	0.737587240087881	1.4024573177807131
9	0.5871121457186238	1.2558849183057714
>10	7.632330916526922	42.091986973320374
>50	1.13896590067398	18.408938726461198
>100	0.2326839730293339	7.162245611081648
>500	0.0018525794030997238	0.3418602680677496
>1k	0.0	0.0
>5k	3.705158806199447E-4	0.5176338628805338
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5878	0.5129243318807167	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03490468403407395	0.0
2	0.0	0.0	0.0	0.1292345926361588	0.0
3	0.0	0.0	0.0	0.1818534038175253	0.0
4	0.0	0.0	0.0	0.26545012207913243	0.0
5	0.0	0.0	0.0	0.38971079724043567	0.0
6	0.0	0.0	0.0	0.5965210501423238	0.0
7	0.0	0.0	0.0	0.6853534710090421	0.0
8	0.0	0.0	0.0	0.9415538518191449	0.0
9	0.0	0.0	0.0	1.0596189455644	0.0
10	0.0	0.0	0.0	1.2629387300628807	0.0
11	0.0	0.0	0.0	1.4389455993046987	0.0
12	0.0	0.0	0.0	1.5737649413863093	0.0
13	0.0	0.0	0.0	1.6473265629881202	0.0
14	0.0	0.0	0.0	1.6889503986987533	0.0
15	0.0	0.0	0.0	1.7313595898001533	0.0
16	0.0	0.0	0.0	1.8084116798053715	0.0
17	0.0	0.0	0.0	1.8877325742728046	0.0
18	0.0	0.0	0.0	1.9958498330683485	0.0
19	0.0	0.0	0.0	2.0480323356992893	0.0
20	0.0	0.0	0.0	2.111209813800963	0.0
21	0.0	0.0	0.0	2.188523688936437	0.0
22	0.0	0.0	0.0	2.266884704592933	0.0
23	0.0	0.0	0.0	2.3591203321529735	0.0
24	0.0	0.0	0.0	2.4357361136077658	0.0
25	0.0	0.0	0.0	2.5045856028649767	0.0
26	0.0	0.0	0.0	2.5693337917481838	0.0
27	0.0	0.0	0.0	2.644204339001272	0.0
28	0.0	0.0	0.0	2.7103487152458423	0.0
29	0.0	0.0	0.0	2.790891273654468	0.0
30	0.0	0.0	0.0	2.896477942857542	0.0
31	0.0	0.0	0.0	2.9788529971779565	0.0
32	0.0	0.0	0.0	3.062711500569819	0.0
33	0.0	0.0	0.0	3.1482279764533003	0.0
34	0.0	0.0	0.0	3.234529807727548	0.0
35	0.0	0.0	0.0	3.337934934178492	0.0
36	0.0	0.0	0.0	3.427203663595636	0.0
37	0.0	0.0	0.0	3.525634872571725	0.0
38	0.0	0.0	0.0	3.6402094979135726	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATACGG	80	0.0	41.25	2
CGTTTTT	4135	0.0	41.233376	1
ACATACG	420	0.0	39.809525	17
GCGATAA	100	0.0	39.600002	9
TACGGGA	195	0.0	39.48718	4
TACGGGT	145	0.0	39.448277	4
TCGTTAG	40	4.124522E-7	38.5	1
CGAAAGG	235	0.0	38.38298	2
CATACGA	430	0.0	38.372093	18
TGGGCGA	845	0.0	38.011833	6
GGGCGAT	2570	0.0	37.92218	7
ACGAGTA	35	7.2901876E-6	37.714287	37
ATAGGGC	545	0.0	37.541283	4
TAGGACG	65	1.0913936E-11	37.230766	1
TAGGGTA	545	0.0	37.137615	5
GCGATAT	95	0.0	37.05263	9
TAAGGGA	1050	0.0	36.87619	4
GGACTAA	895	0.0	36.871506	8
GCGATGA	275	0.0	36.8	9
TTACGGG	335	0.0	36.776115	3
>>END_MODULE