FastQCFastQC Report
Thu 26 May 2016
SRR1546648_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546648_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1277978
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46800.36620348707098244No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA12960.10141019641965666No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAG207.856812E-444.0000041
GAGTACG800.044.0000041
TTCGTAG800.044.0000041
AAGCGAT207.856812E-444.00000422
GTTACGC207.856812E-444.00000441
TCGACAT207.856812E-444.00000417
TATCGCG408.314601E-944.0000041
CGCCCAT207.856812E-444.00000432
TCCGGTA207.856812E-444.00000417
TAATCCG207.856812E-444.0000041
ACGCGAG700.044.01
GTTAGCG351.4462603E-744.01
GAACCGG700.044.02
GTGCGCC254.4431217E-544.041
TAATGCG1200.044.01
GCGTAAT302.5279405E-644.033
CGACGGT1000.041.828
CGTTTTT54400.041.7352941
AGGCGAT800.041.2500046
CGTTAGG1550.041.1612932
Produced by FastQC (version 0.11.5)