##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546648_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1277978
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.98949042941271	31.0	31.0	33.0	30.0	34.0
2	31.363853681362276	31.0	31.0	34.0	30.0	34.0
3	31.49420256060746	31.0	31.0	34.0	30.0	34.0
4	35.26438952783225	37.0	35.0	37.0	33.0	37.0
5	35.03985436369014	37.0	35.0	37.0	32.0	37.0
6	35.132068783656685	37.0	35.0	37.0	32.0	37.0
7	35.70881188878056	37.0	35.0	37.0	35.0	37.0
8	35.76174550735615	37.0	35.0	37.0	35.0	37.0
9	37.52283998629084	39.0	37.0	39.0	35.0	39.0
10	36.76410626787003	39.0	37.0	39.0	32.0	39.0
11	36.32175358261253	38.0	35.0	39.0	32.0	39.0
12	35.691910971863365	37.0	35.0	39.0	30.0	39.0
13	35.431617758678165	37.0	35.0	39.0	30.0	39.0
14	36.36513461108094	38.0	35.0	40.0	30.0	41.0
15	36.632772238645735	38.0	35.0	40.0	31.0	41.0
16	36.724806686813075	38.0	35.0	40.0	31.0	41.0
17	36.65433599013441	38.0	35.0	40.0	31.0	41.0
18	36.57753106860994	38.0	35.0	40.0	31.0	41.0
19	36.539182990630515	38.0	35.0	40.0	31.0	41.0
20	36.39500288737365	38.0	35.0	40.0	31.0	41.0
21	36.2643112792239	38.0	34.0	40.0	30.0	41.0
22	36.19044850537333	38.0	34.0	40.0	30.0	41.0
23	36.01530621027905	38.0	34.0	40.0	30.0	41.0
24	35.99506642524363	37.0	34.0	40.0	30.0	41.0
25	35.84481814240934	37.0	34.0	40.0	30.0	41.0
26	35.747942452843475	37.0	34.0	40.0	30.0	41.0
27	35.56713339353259	37.0	34.0	40.0	29.0	41.0
28	35.568893987220434	37.0	34.0	40.0	29.0	41.0
29	35.484387837662304	37.0	34.0	40.0	29.0	41.0
30	35.43210133507775	37.0	34.0	40.0	29.0	41.0
31	35.32599622215719	37.0	34.0	40.0	28.0	41.0
32	35.072853366802875	37.0	34.0	40.0	27.0	41.0
33	34.84162090427222	37.0	33.0	40.0	25.0	41.0
34	34.737238825707486	37.0	34.0	40.0	25.0	41.0
35	34.50767305853466	37.0	33.0	40.0	24.0	41.0
36	34.27952280868685	37.0	33.0	40.0	23.0	41.0
37	34.30682766057006	37.0	33.0	40.0	23.0	41.0
38	34.29030859686161	37.0	33.0	40.0	23.0	41.0
39	34.216670396516996	37.0	33.0	40.0	23.0	41.0
40	34.15577576452803	37.0	33.0	40.0	23.0	41.0
41	34.20753017657581	37.0	33.0	40.0	23.0	41.0
42	33.98035177444369	36.0	33.0	40.0	23.0	41.0
43	34.02857091436629	37.0	33.0	40.0	23.0	41.0
44	33.84178835629408	36.0	33.0	40.0	22.0	41.0
45	33.6167970027653	36.0	32.0	40.0	21.0	41.0
46	33.738446201734305	36.0	33.0	40.0	22.0	41.0
47	33.63848908197168	36.0	32.0	40.0	22.0	41.0
48	33.71632140772376	36.0	33.0	40.0	22.0	41.0
49	33.66242298380723	36.0	33.0	40.0	22.0	41.0
50	33.15838066069995	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	3.0
14	16.0
15	34.0
16	132.0
17	360.0
18	816.0
19	1652.0
20	2774.0
21	4648.0
22	6848.0
23	9857.0
24	13653.0
25	18874.0
26	23552.0
27	27940.0
28	30815.0
29	34804.0
30	41173.0
31	48944.0
32	60048.0
33	74402.0
34	111436.0
35	147783.0
36	97817.0
37	118512.0
38	164367.0
39	236717.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.465095643273983	25.57735735669941	28.779055664495008	14.178491335531598
2	30.47423351575692	26.600066667814314	30.043396678190078	12.882303138238688
3	27.479268031218062	26.13159224963184	33.52397302613973	12.865166693010366
4	23.49156245256178	29.646910979688222	32.67317590756648	14.188350660183508
5	21.750530916807644	33.90074007533776	31.388959747350892	12.9597692605037
6	20.891752440182852	42.19462306862872	26.757189873378103	10.156434617810323
7	89.41914493050741	3.5556167633558635	6.067710085776124	0.9575282203606009
8	90.2732284906313	2.719139140110393	5.638203474551205	1.3694288947071076
9	84.69543294172513	5.855812854368385	7.411942928595015	2.0368112753114684
10	41.14030131974103	37.17520958889746	12.221650137952295	9.462838953409214
11	33.10808167276745	24.616933937829916	29.471242853945846	12.803741535456792
12	32.567618534904355	24.361843474613803	28.21097076788489	14.859567222596946
13	20.63454926454133	36.22394125720474	28.656518343821258	14.484991134432674
14	16.514525289167732	38.98541289443167	28.737896896503695	15.762164919896898
15	14.945562443171948	26.914547824766938	42.92969049545454	15.210199236606577
16	16.50051878827335	23.852132039831673	42.56450424029208	17.082844931602892
17	17.442553784180948	23.406975706937054	31.39537613323547	27.75509437564653
18	19.17810791735069	25.720161066935425	35.86110246029274	19.24062855542114
19	24.56075143703569	26.143094795059067	30.33455974985485	18.96159401805039
20	26.731915572881537	25.729472651328894	29.240644205142814	18.297967570646755
21	21.127828491570277	29.795426838333682	31.14365036017834	17.9330943099177
22	21.456316149417283	27.109778102596444	28.921624628905974	22.512281119080296
23	17.731917137853703	31.38700353214218	28.688365527419098	22.192713802585022
24	17.651712314296493	26.438639788791356	39.20490024084921	16.70474765606294
25	16.207477750008216	28.276699598897633	36.51768653294501	18.99813611814914
26	16.316712807262725	34.41835461956309	30.681279333447055	18.583653239727134
27	15.863418619099859	35.6677501490636	30.646771697165363	17.822059534671176
28	15.892918344447244	31.183791896261127	36.63412046216758	16.28916929712405
29	15.902151680232368	26.4104702897859	37.11143697309343	20.575941056888304
30	17.3927876692713	32.48060608242083	33.01285311640733	17.11375313190055
31	24.034138302850284	29.38219593764525	28.764736169167232	17.818929590337234
32	25.39730730888951	28.614107598096368	29.396593681581372	16.591991411432748
33	25.19229595501644	29.90716585105534	27.629974850897277	17.270563343030947
34	19.66504900710341	28.237027554464945	30.15239698961954	21.945526448812107
35	19.07114206973829	28.435231279411692	32.15759582715821	20.3360308236918
36	26.28245556652775	27.6496935002011	28.60158782076061	17.466263112510543
37	19.608475263267444	33.105812463125346	30.442777575200825	16.84293469840639
38	19.354871523609955	33.6065253079474	27.814250323557992	19.224352844884653
39	20.04478950341868	33.04070962097939	28.612777371754444	18.301723503847484
40	23.070350193821803	28.250721060925933	28.36770273040694	20.311226014845328
41	18.72301401119581	26.628783907078212	30.042927186539988	24.60527489518599
42	20.3228068088809	27.553525960540792	27.864798924551128	24.258868306027175
43	20.315294942479447	28.110030063115328	28.537971702173277	23.036703292231948
44	18.570976965174673	30.97408562588714	30.561950205715593	19.89298720322259
45	17.491380915790415	36.11353247082501	26.745530830734175	19.649555782650406
46	20.13829659039514	33.079051439070156	28.686252814993686	18.096399155541018
47	20.193383610672484	29.230080643015764	29.516940041221368	21.059595705090384
48	20.76185975032434	26.571114682725366	32.478571618603766	20.18845394834653
49	21.08174006125301	26.89506392128816	32.74759033410591	19.27560568335292
50	19.20471244418918	32.507680100909404	29.404340293807874	18.88326716109354
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1573.0
1	1767.0
2	1961.0
3	5852.5
4	9744.0
5	7797.0
6	5850.0
7	6364.5
8	6879.0
9	7466.5
10	8054.0
11	8490.0
12	8926.0
13	8833.0
14	8740.0
15	8608.0
16	8476.0
17	8515.5
18	8555.0
19	8738.0
20	8921.0
21	9082.5
22	9244.0
23	10166.0
24	11088.0
25	13946.5
26	16805.0
27	19615.0
28	22425.0
29	26128.0
30	29831.0
31	33184.5
32	36538.0
33	41999.0
34	47460.0
35	52747.0
36	58034.0
37	60431.0
38	62828.0
39	71645.5
40	80463.0
41	96626.5
42	112790.0
43	120857.5
44	128925.0
45	126062.0
46	123199.0
47	116514.5
48	109830.0
49	101497.5
50	93165.0
51	83084.5
52	73004.0
53	63802.0
54	54600.0
55	47407.5
56	40215.0
57	34197.5
58	28180.0
59	24415.5
60	20651.0
61	18647.0
62	16643.0
63	13298.0
64	9953.0
65	8172.0
66	6391.0
67	4784.5
68	3178.0
69	2745.5
70	2313.0
71	1622.5
72	932.0
73	789.5
74	647.0
75	506.5
76	366.0
77	284.5
78	203.0
79	202.5
80	202.0
81	134.0
82	66.0
83	58.5
84	51.0
85	46.5
86	42.0
87	34.0
88	26.0
89	14.5
90	3.0
91	2.5
92	2.0
93	4.0
94	6.0
95	3.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1277978.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.082941542967422
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.6319698368287	19.177720745316908
2	5.929457936682021	2.8559757774119467
3	2.3277988518055723	1.6818073101546087
4	1.4020994074263142	1.3506671226590876
5	0.9979543802376348	1.201683850090562
6	0.7540081592269271	1.0895240652949536
7	0.617261794501916	1.040583579958777
8	0.5221920944334838	1.006073734755305
9	0.45670920866692916	0.9899011057014508
>10	5.547092897555576	30.8697623632587
>50	1.3229602597083896	22.046944083477005
>100	0.48918368850687804	16.07882975944087
>500	3.278711048972478E-4	0.040901801381859136
>1k	9.836133146917435E-4	0.5696247010979378
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4680	0.36620348707098244	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1296	0.10141019641965666	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03325565854811272	0.0
2	0.0	0.0	0.0	0.1337268716675874	0.0
3	0.0	0.0	0.0	0.18623168786943123	0.0
4	0.0	0.0	0.0	0.2726181514861758	0.0
5	0.0	0.0	0.0	0.4155001103305378	0.0
6	0.0	0.0	0.0	0.7114363471045667	0.0
7	0.0	0.0	0.0	0.8486844061478367	0.0
8	0.0	0.0	0.0	1.2593331027607675	0.0
9	0.0	0.0	0.0	1.495800397189936	0.0
10	0.0	0.0	0.0	1.8760886337636484	0.0
11	0.0	0.0	0.0	2.2008203584099255	0.0
12	0.0	0.0	0.0	2.4714040460790403	0.0
13	0.0	0.0	0.0	2.6214848768914645	0.0
14	0.0	0.0	0.0	2.693004104921994	0.0
15	0.0	0.0	0.0	2.7606109025350984	0.0
16	0.0	0.0	0.0	2.882287488517017	0.0
17	0.0	0.0	0.0	3.0153101227094674	0.0
18	0.0	0.0	0.0	3.2011505675371565	0.0
19	0.0	0.0	0.0	3.2854243187284915	0.0
20	0.0	0.0	0.0	3.3817483556054957	0.0
21	0.0	0.0	0.0	3.493878611368897	0.0
22	0.0	0.0	0.0	3.6154769487424665	0.0
23	7.824860834850052E-5	0.0	0.0	3.7491255718017054	0.0
24	7.824860834850052E-5	0.0	0.0	3.8603168442649247	0.0
25	7.824860834850052E-5	0.0	0.0	3.9492855119571697	0.0
26	7.824860834850052E-5	0.0	0.0	4.038332428257763	0.0
27	7.824860834850052E-5	0.0	0.0	4.131291774975782	0.0
28	7.824860834850052E-5	0.0	0.0	4.224955359168937	0.0
29	7.824860834850052E-5	0.0	0.0	4.326522052805291	0.0
30	7.824860834850052E-5	0.0	0.0	4.468856271391213	0.0
31	7.824860834850052E-5	0.0	0.0	4.582707996538281	0.0
32	7.824860834850052E-5	0.0	0.0	4.69499474951838	0.0
33	7.824860834850052E-5	0.0	0.0	4.796404945938036	0.0
34	7.824860834850052E-5	0.0	0.0	4.909004693351529	0.0
35	7.824860834850052E-5	0.0	0.0	5.03936687486013	0.0
36	7.824860834850052E-5	0.0	0.0	5.15681803599123	0.0
37	7.824860834850052E-5	0.0	0.0	5.2764601581560875	0.0
38	7.824860834850052E-5	0.0	0.0	5.4003277051717635	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAG	20	7.856812E-4	44.000004	1
GAGTACG	80	0.0	44.000004	1
TTCGTAG	80	0.0	44.000004	1
AAGCGAT	20	7.856812E-4	44.000004	22
GTTACGC	20	7.856812E-4	44.000004	41
TCGACAT	20	7.856812E-4	44.000004	17
TATCGCG	40	8.314601E-9	44.000004	1
CGCCCAT	20	7.856812E-4	44.000004	32
TCCGGTA	20	7.856812E-4	44.000004	17
TAATCCG	20	7.856812E-4	44.000004	1
ACGCGAG	70	0.0	44.0	1
GTTAGCG	35	1.4462603E-7	44.0	1
GAACCGG	70	0.0	44.0	2
GTGCGCC	25	4.4431217E-5	44.0	41
TAATGCG	120	0.0	44.0	1
GCGTAAT	30	2.5279405E-6	44.0	33
CGACGGT	100	0.0	41.8	28
CGTTTTT	5440	0.0	41.735294	1
AGGCGAT	80	0.0	41.250004	6
CGTTAGG	155	0.0	41.161293	2
>>END_MODULE