FastQCFastQC Report
Thu 26 May 2016
SRR1546647_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546647_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1946116
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88100.45269655046256235No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA27520.1414098645712794No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20160.10359094730221631No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT68350.040.0087781
CGGTCTA1600.038.50000431
TATAGCG1350.037.481481
GCGATCG1300.037.230779
CGTAAGG2800.036.9285742
TAATACG601.9826984E-1036.6666641
GGGCGAT44000.036.37
GTAGGGC10400.036.173084
TAGCGGG11400.035.8947333
AGGGCGA21050.035.7434656
TAGGGAT17400.035.655175
TCACGAC1550.035.4838725
GGCGATA7100.035.3239488
AGTAGGG29500.035.2745743
TTTAGCG751.8189894E-1235.2000051
CGGGTAT2000.035.26
GCACGAC250.002353144635.225
TTAACGC250.002353144635.215
CGTCCAA250.002353144635.222
CTAGCGG2950.035.0508462
Produced by FastQC (version 0.11.5)