##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546647_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1946116
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.93876264313124	31.0	31.0	33.0	30.0	34.0
2	31.309291943542934	31.0	31.0	34.0	30.0	34.0
3	31.466760460321996	31.0	31.0	34.0	30.0	34.0
4	35.25716349898978	37.0	35.0	37.0	33.0	37.0
5	35.05048619917826	37.0	35.0	37.0	32.0	37.0
6	35.11228878443011	37.0	35.0	37.0	32.0	37.0
7	35.62016704040253	37.0	35.0	37.0	35.0	37.0
8	35.64330697656255	37.0	35.0	37.0	35.0	37.0
9	37.38667684762881	39.0	37.0	39.0	35.0	39.0
10	36.65578927463728	39.0	35.0	39.0	32.0	39.0
11	36.287719231536045	38.0	35.0	39.0	32.0	39.0
12	35.742354001508645	37.0	35.0	39.0	30.0	39.0
13	35.57375665171038	37.0	35.0	39.0	30.0	39.0
14	36.489958974696265	38.0	35.0	40.0	30.0	41.0
15	36.67760297947296	38.0	35.0	40.0	31.0	41.0
16	36.73722070010215	38.0	35.0	40.0	31.0	41.0
17	36.63246024389091	38.0	35.0	40.0	31.0	41.0
18	36.53498249847388	38.0	35.0	40.0	31.0	41.0
19	36.48620328901258	38.0	35.0	40.0	30.0	41.0
20	36.34910200625245	38.0	35.0	40.0	30.0	41.0
21	36.22364699740406	38.0	34.0	40.0	30.0	41.0
22	36.14851684072275	38.0	34.0	40.0	30.0	41.0
23	35.958367846520964	38.0	34.0	40.0	30.0	41.0
24	35.89255727818897	38.0	34.0	40.0	30.0	41.0
25	35.70912268333439	37.0	34.0	40.0	29.0	41.0
26	35.57742755313661	37.0	34.0	40.0	29.0	41.0
27	35.39182299513492	37.0	34.0	40.0	28.0	41.0
28	35.324926160619405	37.0	34.0	40.0	28.0	41.0
29	35.17063371350937	37.0	34.0	40.0	27.0	41.0
30	35.13615735136035	37.0	34.0	40.0	27.0	41.0
31	35.053421789862476	37.0	34.0	40.0	27.0	41.0
32	34.850269459785544	37.0	33.0	40.0	25.0	41.0
33	34.63844909553182	37.0	33.0	40.0	25.0	41.0
34	34.493654540633756	37.0	33.0	40.0	24.0	41.0
35	34.25847277346263	37.0	33.0	40.0	23.0	41.0
36	34.05110589502373	36.0	33.0	40.0	23.0	41.0
37	34.077558069508704	37.0	33.0	40.0	23.0	41.0
38	34.00039977062004	36.0	33.0	40.0	23.0	41.0
39	33.8830958688999	36.0	33.0	40.0	22.0	41.0
40	33.837598580968454	36.0	33.0	40.0	22.0	41.0
41	33.8232510292295	36.0	33.0	40.0	22.0	41.0
42	33.56550380347318	36.0	32.0	40.0	21.0	41.0
43	33.58383981222085	36.0	32.0	40.0	21.0	41.0
44	33.36930378250834	35.0	32.0	40.0	20.0	41.0
45	33.18714197920371	35.0	32.0	40.0	20.0	41.0
46	33.239862372027154	35.0	32.0	40.0	20.0	41.0
47	33.09138150038333	35.0	32.0	39.0	20.0	41.0
48	33.1178840315788	35.0	32.0	39.0	20.0	41.0
49	33.01466921807333	35.0	31.0	39.0	20.0	41.0
50	32.51042538060424	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	0.0
12	2.0
13	2.0
14	15.0
15	83.0
16	219.0
17	619.0
18	1424.0
19	2847.0
20	4804.0
21	7657.0
22	11181.0
23	16140.0
24	22428.0
25	30200.0
26	37419.0
27	44125.0
28	50025.0
29	57238.0
30	66910.0
31	79439.0
32	95007.0
33	117236.0
34	169725.0
35	210151.0
36	166594.0
37	196540.0
38	250720.0
39	307363.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.60745197100275	24.092140447948633	28.446968217721864	13.85343936332675
2	31.418116905672633	26.513578841137935	28.560784660318294	13.507519592871134
3	28.58868638868392	26.5839754670328	31.01259123299947	13.814746911283809
4	25.241198366387202	29.54638880724479	30.464473854590373	14.747938971777632
5	23.333141498245737	32.926557306964234	29.49803608829073	14.242265106499305
6	21.504370756933298	42.09846689508744	26.287384719102047	10.109777628877211
7	86.63764133278798	4.675980260169486	7.066433861085362	1.6199445459571784
8	86.29978891289112	4.223026787714606	6.745075833095253	2.7321084662990285
9	80.40753994109292	6.50767991219434	9.550972295587725	3.53380785112501
10	43.249169114276846	26.667834805325068	16.377595169044394	13.70540091135369
11	33.93107091252526	26.168943680643906	24.559224629980946	15.34076077684989
12	30.914241494340523	23.59807945672303	28.22920113703397	17.258477911902474
13	23.00921425033246	30.51164473237978	29.435758197353085	17.04338281993468
14	19.44508960411404	31.928620904406518	30.87642257707146	17.74986691440798
15	18.39068174764505	24.6727327661866	39.67096514287946	17.26562034328889
16	20.605246552620706	23.158794234259418	37.35460784454781	18.88135136857207
17	20.747427183168938	23.531999120299098	30.272142051141866	25.4484316453901
18	22.87253175041981	24.729255604496338	32.673694682125834	19.724517962958014
19	26.475297464282704	26.34113279989477	27.72450357532644	19.459066160496086
20	28.063897527177208	25.882938118796616	26.82712644056161	19.226037913464562
21	24.635838768089876	28.363108879429593	28.58652824394846	18.41452410853207
22	23.430823239724663	25.997782249362324	27.127314096384797	23.44408041452822
23	21.66355962337291	28.71766122882706	27.11739690748136	22.501382240318666
24	21.36553011228519	25.532804827667004	34.05393100925125	19.04773405079656
25	20.863144848508515	26.06894964123413	31.907399147841137	21.160506362416218
26	19.428646596605752	30.43076568919838	28.91549116291115	21.225096551284715
27	20.083797677014115	30.80412472843345	28.670490351037657	20.441587243514775
28	19.639630936696477	28.77212869119826	32.455670679445625	19.132569692659636
29	20.07680939882309	26.331421148585182	32.19366163168074	21.398107820910983
30	21.308904505178518	29.009987071685345	30.27250174193111	19.408606681205026
31	25.354141274209756	27.484178743713116	27.428734977771107	19.732945004306014
32	26.844854058031487	26.008829895031948	27.868739581813212	19.277576465123353
33	26.011912958939753	26.49728998682504	27.466553895040175	20.02424315919503
34	22.146161893741176	27.051265186658966	28.343839730005815	22.458733189594042
35	21.44959498817131	26.775639273301284	30.35882753134962	21.415938207177785
36	26.640292767748686	26.45238002256803	26.67297324517141	20.23435396451188
37	22.1111691183876	29.932182870908004	28.144211341975506	19.812436668728893
38	21.99051855079553	29.543819587321618	26.36965114104195	22.096010720840894
39	21.500773849040858	29.212441601631145	27.183785550296076	22.102998999031918
40	24.166082597337464	25.343350550532445	27.628260597004495	22.862306255125596
41	19.725853957318062	25.529773148157663	28.856039413888997	25.88833348063528
42	22.02247964663977	25.56928775057602	27.35140145808369	25.05683114470052
43	21.556782843365966	25.26272842934337	28.913795477761862	24.266693249528807
44	20.925628277040012	27.425908835855623	29.265829991634618	22.382632895469744
45	19.90014983690592	30.195322375439083	27.26014276641269	22.64438502124231
46	21.516394706173735	29.3936229906131	27.434181724008226	21.65580057920494
47	21.316046936564934	27.281467291775	28.72187474950106	22.68061102215901
48	21.953059324315713	26.075680997432837	30.41730297680097	21.55395670145048
49	22.31603871506118	25.141512633368208	30.587231182519435	21.955217469051178
50	21.049824368126053	28.478569622776856	28.77279668837829	21.698809320718805
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2694.0
1	3315.5
2	3937.0
3	10563.0
4	17189.0
5	13156.5
6	9124.0
7	10001.5
8	10879.0
9	11901.5
10	12924.0
11	13461.0
12	13998.0
13	13994.5
14	13991.0
15	13805.0
16	13619.0
17	13566.0
18	13513.0
19	13385.5
20	13258.0
21	13534.5
22	13811.0
23	14609.5
24	15408.0
25	17053.5
26	18699.0
27	21393.5
28	24088.0
29	27560.5
30	31033.0
31	35863.0
32	40693.0
33	45055.0
34	49417.0
35	54949.0
36	60481.0
37	65308.5
38	70136.0
39	78703.5
40	87271.0
41	103752.0
42	120233.0
43	136303.0
44	152373.0
45	153716.5
46	155060.0
47	153372.0
48	151684.0
49	147889.5
50	144095.0
51	128960.0
52	113825.0
53	103392.0
54	92959.0
55	88858.0
56	84757.0
57	81690.0
58	78623.0
59	76004.0
60	73385.0
61	70234.0
62	67083.0
63	60500.5
64	53918.0
65	46714.0
66	39510.0
67	33059.0
68	26608.0
69	23719.5
70	20831.0
71	17490.0
72	14149.0
73	11424.5
74	8700.0
75	7102.5
76	5505.0
77	4380.5
78	3256.0
79	2508.5
80	1761.0
81	1327.5
82	894.0
83	694.5
84	495.0
85	310.5
86	126.0
87	96.0
88	66.0
89	47.5
90	29.0
91	22.5
92	16.0
93	13.5
94	11.0
95	6.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1946116.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.20283708226151
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.97095998668118	19.90292238774118
2	6.149609326719737	3.099752039617469
3	2.3902433734608297	1.807227429848653
4	1.4932083896582315	1.505323510976899
5	1.054343773790941	1.3286227179774934
6	0.8139422120083895	1.2308191778173798
7	0.6843299792703549	1.2072939884640705
8	0.549756492368935	1.1084338649671848
9	0.4705332313358378	1.0672895134032467
>10	5.843173945954372	34.00552934045052
>50	1.2620211781711999	21.691235623806442
>100	0.31477585402402686	10.863122507530477
>500	0.001861353933597817	0.30200516806392286
>1k	0.001034085518665454	0.42121224769150156
>5k	2.068171037330908E-4	0.45921048164362444
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8810	0.45269655046256235	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2752	0.1414098645712794	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2016	0.10359094730221631	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07358245859959016	0.0
2	0.0	0.0	0.0	0.2283522667713538	0.0
3	0.0	0.0	0.0	0.31925126765311007	0.0
4	0.0	0.0	0.0	0.4771555241311412	0.0
5	0.0	0.0	0.0	0.7493900671902395	0.0
6	0.0	0.0	0.0	1.3257174803557445	0.0
7	0.0	0.0	0.0	1.620920849527983	0.0
8	0.0	0.0	0.0	2.4235451535263057	0.0
9	0.0	0.0	0.0	2.873261408877991	0.0
10	0.0	0.0	0.0	3.5368395306343507	0.0
11	0.0	0.0	0.0	4.000480957969617	0.0
12	0.0	0.0	0.0	4.393006377831537	0.0
13	0.0	0.0	0.0	4.605686403071553	0.0
14	0.0	0.0	0.0	4.7182696201048655	0.0
15	0.0	0.0	0.0	4.814872289216059	0.0
16	0.0	0.0	0.0	4.983413116176014	0.0
17	0.0	0.0	0.0	5.154369009863749	0.0
18	0.0	0.0	0.0	5.411599308571534	0.0
19	5.138439846340095E-5	0.0	0.0	5.526494823535699	0.0
20	5.138439846340095E-5	0.0	0.0	5.6551099728895915	0.0
21	5.138439846340095E-5	0.0	0.0	5.795594918288529	0.0
22	5.138439846340095E-5	0.0	0.0	5.944558289433929	0.0
23	5.138439846340095E-5	0.0	0.0	6.099636403996473	0.0
24	5.138439846340095E-5	0.0	0.0	6.227840478162658	0.0
25	5.138439846340095E-5	0.0	0.0	6.346743976206968	0.0
26	5.138439846340095E-5	0.0	0.0	6.4585564272633285	0.0
27	5.138439846340095E-5	0.0	0.0	6.58095406440315	0.0
28	5.138439846340095E-5	0.0	0.0	6.706023690263067	0.0
29	5.138439846340095E-5	0.0	0.0	6.835255452398521	0.0
30	5.138439846340095E-5	0.0	0.0	7.014946693825034	0.0
31	5.138439846340095E-5	0.0	0.0	7.15825778113946	0.0
32	5.138439846340095E-5	0.0	0.0	7.296173506615228	0.0
33	5.138439846340095E-5	0.0	0.0	7.429156329838509	0.0
34	5.138439846340095E-5	0.0	0.0	7.56635267373579	0.0
35	5.138439846340095E-5	0.0	0.0	7.7255415401754055	0.0
36	5.138439846340095E-5	0.0	0.0	7.870085853052952	0.0
37	5.138439846340095E-5	0.0	0.0	8.022903054083107	0.0
38	5.138439846340095E-5	0.0	0.0	8.188926045518356	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	6835	0.0	40.008778	1
CGGTCTA	160	0.0	38.500004	31
TATAGCG	135	0.0	37.48148	1
GCGATCG	130	0.0	37.23077	9
CGTAAGG	280	0.0	36.928574	2
TAATACG	60	1.9826984E-10	36.666664	1
GGGCGAT	4400	0.0	36.3	7
GTAGGGC	1040	0.0	36.17308	4
TAGCGGG	1140	0.0	35.894733	3
AGGGCGA	2105	0.0	35.743465	6
TAGGGAT	1740	0.0	35.65517	5
TCACGAC	155	0.0	35.48387	25
GGCGATA	710	0.0	35.323948	8
AGTAGGG	2950	0.0	35.274574	3
TTTAGCG	75	1.8189894E-12	35.200005	1
CGGGTAT	200	0.0	35.2	6
GCACGAC	25	0.0023531446	35.2	25
TTAACGC	25	0.0023531446	35.2	15
CGTCCAA	25	0.0023531446	35.2	22
CTAGCGG	295	0.0	35.050846	2
>>END_MODULE