FastQCFastQC Report
Thu 26 May 2016
SRR1546646_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546646_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3663052
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118190.32265444225198003No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT102900.041.306121
TACGGGA18800.039.4361724
AGGGCGA34300.037.9067046
GGGCGAT67850.037.255717
AAGGGAT92700.037.188785
ACGGGAT28050.037.176475
TTAATCG11800.037.10169620
TAGGGAT94600.037.093025
TAATCGT11850.036.9451521
GGCGATT21400.036.9065448
CGAGGGA26300.036.8897364
TAGGGAC42250.036.81425
AGGGATT108100.036.7548566
TAAGGGA65500.036.711454
ACGTAAG1200.036.6666641
ATAGGGA67900.036.6450654
TTAGGGA58950.036.6106834
CGGGATA12750.036.580396
CGTTGAT12150.036.5761325
AGGGATC66750.036.551316
Produced by FastQC (version 0.11.5)