##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546646_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3663052
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.951756895616004	31.0	31.0	33.0	30.0	34.0
2	31.32214857992734	31.0	31.0	34.0	30.0	34.0
3	31.497724029033712	31.0	31.0	34.0	30.0	34.0
4	35.2726344043164	37.0	35.0	37.0	33.0	37.0
5	35.063889892908975	37.0	35.0	37.0	32.0	37.0
6	35.13567675260957	37.0	35.0	37.0	32.0	37.0
7	35.69114443365805	37.0	35.0	37.0	35.0	37.0
8	35.735950786393424	37.0	35.0	37.0	35.0	37.0
9	37.50995017269752	39.0	37.0	39.0	35.0	39.0
10	36.70677484239918	39.0	37.0	39.0	32.0	39.0
11	36.26538471198334	38.0	35.0	39.0	31.0	39.0
12	35.92950659723094	37.0	35.0	39.0	31.0	39.0
13	35.84262549371398	37.0	35.0	39.0	30.0	39.0
14	36.80572839260813	39.0	35.0	41.0	31.0	41.0
15	36.95917366174436	39.0	35.0	41.0	31.0	41.0
16	36.98726335307279	39.0	35.0	41.0	31.0	41.0
17	36.890298581619916	38.0	35.0	40.0	31.0	41.0
18	36.83093715295333	38.0	35.0	40.0	31.0	41.0
19	36.819440455663745	38.0	35.0	40.0	31.0	41.0
20	36.71504144631307	38.0	35.0	40.0	31.0	41.0
21	36.63082587962169	38.0	35.0	40.0	31.0	41.0
22	36.569068907566695	38.0	35.0	40.0	30.0	41.0
23	36.37432419741789	38.0	35.0	40.0	30.0	41.0
24	36.34616707597927	38.0	35.0	40.0	30.0	41.0
25	36.19778015709305	38.0	34.0	40.0	30.0	41.0
26	36.09074372954574	38.0	34.0	40.0	30.0	41.0
27	35.90209857790717	38.0	34.0	40.0	29.0	41.0
28	35.858726821240865	38.0	34.0	40.0	29.0	41.0
29	35.768562936043494	38.0	34.0	40.0	29.0	41.0
30	35.743252621038415	38.0	34.0	40.0	29.0	41.0
31	35.723236798167214	38.0	34.0	40.0	29.0	41.0
32	35.583354263057146	38.0	34.0	40.0	28.0	41.0
33	35.421323803211095	38.0	34.0	40.0	27.0	41.0
34	35.36803982034653	38.0	34.0	40.0	27.0	41.0
35	35.18155161324491	38.0	34.0	40.0	26.0	41.0
36	35.00293143531678	38.0	34.0	40.0	25.0	41.0
37	35.12585106626933	38.0	34.0	40.0	26.0	41.0
38	35.09562162917698	38.0	34.0	40.0	26.0	41.0
39	35.01395366486744	38.0	34.0	40.0	25.0	41.0
40	35.023561500082444	38.0	34.0	40.0	25.0	41.0
41	35.04022492719186	38.0	34.0	40.0	25.0	41.0
42	34.799454935392674	38.0	33.0	40.0	24.0	41.0
43	34.879526143773006	38.0	33.0	40.0	25.0	41.0
44	34.6966646938127	38.0	33.0	40.0	24.0	41.0
45	34.51989051752473	37.0	33.0	40.0	24.0	41.0
46	34.5991367307917	37.0	33.0	40.0	24.0	41.0
47	34.45030182481712	37.0	33.0	40.0	23.0	41.0
48	34.50738509854624	37.0	33.0	40.0	24.0	41.0
49	34.43364112767168	37.0	33.0	40.0	24.0	41.0
50	33.950594203958886	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	8.0
14	20.0
15	79.0
16	268.0
17	735.0
18	1740.0
19	3491.0
20	5940.0
21	9640.0
22	14818.0
23	21264.0
24	30370.0
25	42071.0
26	54951.0
27	67310.0
28	78849.0
29	93196.0
30	112012.0
31	136014.0
32	165091.0
33	204348.0
34	287019.0
35	357568.0
36	307367.0
37	381054.0
38	529738.0
39	758088.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.46137373971213	24.490261126514174	30.164928043609535	12.883437090164158
2	29.31489916059068	26.080820037498786	30.90196371768678	13.702317084223756
3	27.73632479145805	26.43817232187804	32.061761612993756	13.763741273670153
4	24.119286321897697	29.64317732863197	31.80708327372912	14.430453075741212
5	22.065561722847505	34.15946593168756	30.360994056322433	13.413978289142497
6	21.367482634699154	42.60728485426906	26.28624982664729	9.738982684384496
7	91.61469725245506	2.6082621813722544	4.7799212241595255	0.9971193420131628
8	92.20278609203473	2.0508854365157796	4.416945213990956	1.3293832574585347
9	86.58421993463375	5.217234153378112	6.087492069454652	2.1110538425334937
10	38.64984717661666	40.394703651490616	11.335056122599406	9.620393049293321
11	25.82212865119032	25.60452322271155	32.68711446083758	15.886233665260555
12	27.635889416803256	23.843396162544238	30.759568796730157	17.761145623922346
13	21.344905832622633	30.013769938291894	31.373947189392887	17.267377039692583
14	18.26962871397949	31.830014971122438	30.650179140236066	19.250177174662003
15	16.99282456268707	26.750916994899338	38.125612194421485	18.13064624799211
16	19.761144531936754	25.467260634028673	36.79800341354695	17.973591420487615
17	19.526558727531032	25.56715001588839	31.13900648967036	23.767284766910215
18	19.923222493155983	26.44617111632595	33.54445964731049	20.08614674320758
19	23.090144502453143	27.290467074996478	29.991629930451435	19.627758492098938
20	25.019519242424078	26.923478017784074	29.689259120536644	18.3677436192552
21	22.241862796378538	29.056453471039994	30.86216084292552	17.83952288965595
22	22.027314927552215	26.70778356408809	29.43010364035236	21.834797868007332
23	19.83761082288758	29.405970758809868	29.602473565758824	21.153944852543727
24	19.14106051456545	26.280407703739943	35.994165520991785	18.584366260702822
25	18.802817978013962	27.818933501353516	32.93420350025061	20.444045020381914
26	19.30081800640559	31.446755328616682	29.555190589704978	19.69723607527275
27	18.423352985434004	31.500208023254928	30.06509326102933	20.011345730281743
28	17.83643803036375	29.650029538210216	33.68134004103682	18.832192390389217
29	18.70118141920999	26.19580612014244	33.3345800168821	21.768432443765473
30	19.80668033104635	29.654179083452814	31.3074179673125	19.23172261818833
31	22.023847873303463	28.495418574456494	29.367860461713345	20.112873090526698
32	23.698162079053205	27.417546898051132	29.881421284764727	19.002869738130936
33	23.05476416933202	28.511552661551075	29.103490750336057	19.330192418780843
34	19.742498877984808	27.930152233711127	30.184474585673364	22.142874302630702
35	19.266966453110683	27.663025258718687	31.51164657231183	21.558361715858798
36	22.811251382726756	28.281498597344513	28.606200512578035	20.3010495073507
37	19.588801906170048	31.122162611942173	30.359547175415475	18.929488306472308
38	18.84349444124735	30.823176957356868	29.87066522670167	20.462663374694106
39	19.447990364319153	31.318556220332116	29.53662683467229	19.69682658067644
40	22.337739131194425	27.883961243247434	29.703673330326737	20.074626295231408
41	18.556466028874283	27.264996511106038	30.708163575073467	23.470373884946213
42	19.392135301382563	27.313617169507832	29.824610734436746	23.46963679467286
43	19.68355895575602	28.372106101687883	29.684754679977242	22.259580262578854
44	19.291618027808504	28.77496688553698	30.605626128157613	21.327788958496903
45	18.73762643828152	30.97777481728351	28.72244237865037	21.562156365784595
46	19.593852339524528	31.021153944852543	28.950612767713917	20.434380947909013
47	19.26071483560703	28.70366022649965	30.0700890951043	21.96553584278902
48	19.633491416447267	27.26870926211258	31.958678173282824	21.13912114815733
49	19.836382339098655	27.820571479738753	31.472717286022693	20.8703288951399
50	19.24201458237557	29.950052579106167	30.442292383509706	20.365640455008556
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2146.0
1	3182.5
2	4219.0
3	13915.5
4	23612.0
5	18193.0
6	12774.0
7	13264.5
8	13755.0
9	14450.0
10	15145.0
11	15498.0
12	15851.0
13	15961.0
14	16071.0
15	16004.0
16	15937.0
17	16057.0
18	16177.0
19	17219.0
20	18261.0
21	20513.5
22	22766.0
23	27443.5
24	32121.0
25	37881.0
26	43641.0
27	54613.5
28	65586.0
29	79606.0
30	93626.0
31	108984.5
32	124343.0
33	139399.0
34	154455.0
35	171473.5
36	188492.0
37	198672.0
38	208852.0
39	222374.5
40	235897.0
41	264236.0
42	292575.0
43	303223.5
44	313872.0
45	310306.5
46	306741.0
47	296440.0
48	286139.0
49	269030.0
50	251921.0
51	226406.5
52	200892.0
53	180275.5
54	159659.0
55	141258.0
56	122857.0
57	114749.5
58	106642.0
59	99438.5
60	92235.0
61	83669.0
62	75103.0
63	63686.0
64	52269.0
65	41331.0
66	30393.0
67	24213.5
68	18034.0
69	14968.5
70	11903.0
71	9697.0
72	7491.0
73	6004.5
74	4518.0
75	3640.5
76	2763.0
77	2156.0
78	1549.0
79	1307.5
80	1066.0
81	749.5
82	433.0
83	285.5
84	138.0
85	107.5
86	77.0
87	48.0
88	19.0
89	16.0
90	13.0
91	16.0
92	19.0
93	11.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3663052.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.49959700314365
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.41511698676074	15.095635831930545
2	7.068251084301903	2.756560953218405
3	2.532778621934617	1.4816448727770768
4	1.427802644473897	1.113663046690551
5	0.9781387595946801	0.9536655812625534
6	0.7779390534142198	0.9101698820750594
7	0.6230888350456966	0.8504986826384533
8	0.5250339819460852	0.8190360848723568
9	0.46353976905202265	0.8134954822300249
>10	5.707238145959361	25.88694923357752
>50	1.590132199253364	21.997692627366984
>100	0.8875198271598306	26.46204317976807
>500	0.002565068327704115	0.3478737897898958
>1k	7.125189799178095E-4	0.18264832816430904
>5k	0.0	0.0
>10k+	1.425037959835619E-4	0.32842242363821217
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11819	0.32265444225198003	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.044416513879682845	0.0
2	0.0	0.0	0.0	0.14646256727996218	0.0
3	0.0	0.0	0.0	0.20643987581939868	0.0
4	0.0	0.0	0.0	0.34356596630350866	0.0
5	0.0	0.0	0.0	0.5548378783593572	0.0
6	0.0	0.0	0.0	0.8751445515924972	0.0
7	0.0	0.0	0.0	1.0379323034453238	0.0
8	0.0	0.0	0.0	1.477511102763488	0.0
9	0.0	0.0	0.0	1.6769076715263664	0.0
10	0.0	0.0	0.0	1.9846838101124418	0.0
11	0.0	0.0	0.0	2.2585810957638603	0.0
12	0.0	0.0	0.0	2.4832025316593924	0.0
13	0.0	0.0	0.0	2.5979156179055063	0.0
14	0.0	0.0	0.0	2.6546442693142223	0.0
15	0.0	0.0	0.0	2.7127925019901435	0.0
16	0.0	0.0	0.0	2.8203257829809676	0.0
17	0.0	0.0	0.0	2.946586616843004	0.0
18	0.0	0.0	0.0	3.115871682957272	0.0
19	0.0	0.0	0.0	3.2092091512760397	0.0
20	0.0	0.0	0.0	3.304785190054632	0.0
21	0.0	0.0	0.0	3.429599143009709	0.0
22	0.0	0.0	0.0	3.5724581578421493	0.0
23	0.0	0.0	0.0	3.7313693608499143	0.0
24	0.0	0.0	0.0	3.856783905879578	0.0
25	0.0	0.0	0.0	3.9676477429203842	0.0
26	0.0	0.0	0.0	4.073297348768186	0.0
27	0.0	0.0	0.0	4.181840716429906	0.0
28	0.0	0.0	0.0	4.29786418538421	0.0
29	0.0	0.0	0.0	4.42573569799173	0.0
30	0.0	0.0	0.0	4.595785154019108	0.0
31	0.0	0.0	0.0	4.74183822670276	0.0
32	0.0	0.0	0.0	4.881885378640543	0.0
33	0.0	0.0	0.0	5.022205526975866	0.0
34	0.0	0.0	0.0	5.16874999317509	0.0
35	5.459927950790761E-5	0.0	0.0	5.3352231963947006	0.0
36	5.459927950790761E-5	0.0	0.0	5.489575359563555	0.0
37	5.459927950790761E-5	0.0	0.0	5.651544122223763	0.0
38	5.459927950790761E-5	0.0	0.0	5.8228220620400695	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	10290	0.0	41.30612	1
TACGGGA	1880	0.0	39.436172	4
AGGGCGA	3430	0.0	37.906704	6
GGGCGAT	6785	0.0	37.25571	7
AAGGGAT	9270	0.0	37.18878	5
ACGGGAT	2805	0.0	37.17647	5
TTAATCG	1180	0.0	37.101696	20
TAGGGAT	9460	0.0	37.09302	5
TAATCGT	1185	0.0	36.94515	21
GGCGATT	2140	0.0	36.906544	8
CGAGGGA	2630	0.0	36.889736	4
TAGGGAC	4225	0.0	36.8142	5
AGGGATT	10810	0.0	36.754856	6
TAAGGGA	6550	0.0	36.71145	4
ACGTAAG	120	0.0	36.666664	1
ATAGGGA	6790	0.0	36.645065	4
TTAGGGA	5895	0.0	36.610683	4
CGGGATA	1275	0.0	36.58039	6
CGTTGAT	1215	0.0	36.57613	25
AGGGATC	6675	0.0	36.55131	6
>>END_MODULE