FastQCFastQC Report
Thu 26 May 2016
SRR1546645_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546645_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4243076
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT349540.8237891567344068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT331150.043.189491
ACGCGAG950.041.684211
GTTAGCG2700.039.111111
CGTAAGG8550.038.5964932
AGGGCGA35050.038.4136966
TACGGGA21050.038.3562934
GACCGAT28250.037.847799
CGAGGGA36400.037.5329674
TAGGGCG12550.037.5139435
TAGGGAT125750.037.31695
AAGGGAT124950.037.25655
GGACCGA30200.037.2251668
TAGGGAC58150.037.152195
AGGGATT147150.036.9582066
TTAGGGA88900.036.9223824
GGGACCG34850.036.866577
GGGCGAT70200.036.8547027
AGGGATA81100.036.8113446
TAAGGGA89850.036.7523654
AGGGATC81250.036.5538446
Produced by FastQC (version 0.11.5)