FastQCFastQC Report
Thu 26 May 2016
SRR1546644_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546644_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2215880
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79970.36089499431377153No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA207.8580633E-444.00000426
GCGCGTT254.444182E-544.018
AGACGCG254.444182E-544.01
CGGATTA254.444182E-544.06
AGTCGAA302.5287845E-644.024
CGTTTTT82050.040.9165151
TATTACG557.8216544E-1140.0000041
CGGTCTA557.8216544E-1140.00000431
TCGGTAC557.8216544E-1140.0000041
CGACAGG2000.039.6000022
GCGATAA2900.039.4482779
CATACGA4900.039.06122618
CGCAATC1750.038.9714320
CGAACGG2150.038.883722
TCCGACC404.1268504E-738.50000429
ACTTACG404.1268504E-738.5000041
CGTAATC404.1268504E-738.50000431
GGCGATA10900.038.146798
CGTTAGG3600.037.888892
TAAGGGA31750.037.763784
Produced by FastQC (version 0.11.5)