##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546644_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2215880
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.95662851778977	31.0	31.0	33.0	30.0	34.0
2	31.334369640955288	31.0	31.0	34.0	30.0	34.0
3	31.48634763615358	31.0	31.0	34.0	30.0	34.0
4	35.26367086665343	37.0	35.0	37.0	33.0	37.0
5	35.048705706085165	37.0	35.0	37.0	32.0	37.0
6	35.1197081971948	37.0	35.0	37.0	32.0	37.0
7	35.65699902521797	37.0	35.0	37.0	35.0	37.0
8	35.69756981424987	37.0	35.0	37.0	35.0	37.0
9	37.45652381897937	39.0	37.0	39.0	35.0	39.0
10	36.71186706861383	39.0	37.0	39.0	32.0	39.0
11	36.29045119771829	38.0	35.0	39.0	31.0	39.0
12	35.76045363467336	37.0	35.0	39.0	30.0	39.0
13	35.568695506976916	37.0	35.0	39.0	30.0	39.0
14	36.50715607343358	38.0	35.0	40.0	30.0	41.0
15	36.72126198169576	38.0	35.0	40.0	31.0	41.0
16	36.77813554885643	38.0	35.0	40.0	31.0	41.0
17	36.686223531960216	38.0	35.0	40.0	31.0	41.0
18	36.61991172807192	38.0	35.0	40.0	31.0	41.0
19	36.587660884163405	38.0	35.0	40.0	31.0	41.0
20	36.44392837157247	38.0	35.0	40.0	31.0	41.0
21	36.31928669422532	38.0	34.0	40.0	30.0	41.0
22	36.24245807534704	38.0	34.0	40.0	30.0	41.0
23	36.05024369550698	38.0	34.0	40.0	30.0	41.0
24	36.003885589472354	38.0	34.0	40.0	30.0	41.0
25	35.8658140332509	38.0	34.0	40.0	30.0	41.0
26	35.752258696319295	37.0	34.0	40.0	29.0	41.0
27	35.55755501200426	37.0	34.0	40.0	29.0	41.0
28	35.52408478798491	37.0	34.0	40.0	29.0	41.0
29	35.424247251656226	37.0	34.0	40.0	29.0	41.0
30	35.38839603227611	37.0	34.0	40.0	28.0	41.0
31	35.295832806830695	37.0	34.0	40.0	27.0	41.0
32	35.09060824593389	37.0	34.0	40.0	27.0	41.0
33	34.87531048612741	37.0	33.0	40.0	25.0	41.0
34	34.78263398740004	37.0	34.0	40.0	25.0	41.0
35	34.582204361247	37.0	33.0	40.0	24.0	41.0
36	34.35650125458057	37.0	33.0	40.0	23.0	41.0
37	34.43340253082297	37.0	33.0	40.0	24.0	41.0
38	34.40319195985342	37.0	33.0	40.0	23.0	41.0
39	34.309835821434376	37.0	33.0	40.0	23.0	41.0
40	34.293812841850645	37.0	33.0	40.0	23.0	41.0
41	34.30697104536347	37.0	33.0	40.0	23.0	41.0
42	34.0612668556059	37.0	33.0	40.0	23.0	41.0
43	34.12570942469809	37.0	33.0	40.0	23.0	41.0
44	33.94472805386573	36.0	33.0	40.0	23.0	41.0
45	33.771375254977706	36.0	33.0	40.0	22.0	41.0
46	33.841854703323285	36.0	33.0	40.0	23.0	41.0
47	33.69727196418579	36.0	33.0	40.0	22.0	41.0
48	33.743350722963335	36.0	33.0	40.0	23.0	41.0
49	33.663537285412566	36.0	32.0	40.0	22.0	41.0
50	33.18097505280069	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	3.0
13	4.0
14	15.0
15	64.0
16	215.0
17	643.0
18	1410.0
19	2865.0
20	5060.0
21	7677.0
22	11739.0
23	16797.0
24	23307.0
25	31243.0
26	39373.0
27	46731.0
28	52589.0
29	60890.0
30	71329.0
31	85670.0
32	104565.0
33	129111.0
34	189475.0
35	244196.0
36	178986.0
37	216623.0
38	292952.0
39	402346.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.74742314565771	24.784600249110962	29.873729624347888	14.59424698088344
2	30.938092315468346	26.732088380237194	28.889470548946694	13.440348755347761
3	27.584706753073274	27.089869487517376	31.933814105456975	13.39160965395238
4	24.519784464862717	30.05735870173475	31.34082170514649	14.082035128256043
5	22.83657057241367	33.84614690326191	29.70066068559669	13.61662183872773
6	21.61109807390292	42.15007130350019	26.321551708576273	9.917278914020615
7	89.01587631099157	3.796640612307526	6.001994692853404	1.1854883838475008
8	89.40222394714516	3.1675903027239745	5.740247666841165	1.6899380832897086
9	83.54477679296713	6.130295864396989	7.721447009765872	2.603480332870011
10	41.542096142390385	34.949410617903496	12.992716212069247	10.515777027636876
11	32.16103760131415	25.083759048323916	28.684269906312615	14.070933444049317
12	31.472417278914023	23.687474050941386	28.804718667075836	16.03539000306876
13	21.088010181056735	33.323600555986786	29.923145657707096	15.665243605249382
14	17.87244796649638	35.397674964348255	29.923642074480565	16.806234994674803
15	16.327553838655522	26.01174251313248	41.264915067602935	16.395788580609057
16	18.475368702276295	23.726375074462517	40.53134646280485	17.266909760456343
17	18.895382421430764	23.577630557611425	31.161344477137753	26.365642543820062
18	19.78730797696626	25.203621134718485	35.16291495929382	19.846155929021428
19	25.409949997292276	25.839169991154755	29.624167373684497	19.126712637868476
20	27.069155369424337	25.759968951387258	28.322923624022962	18.847952055165443
21	22.462588226799284	29.463869884650794	29.984972110403092	18.08856977814683
22	22.331443941007638	25.7907016625449	28.80589201581313	23.071962380634332
23	19.545417621892884	30.368792533891725	28.615990035561495	21.469799808653896
24	18.76026680145134	26.122984999187683	37.52865678646858	17.588091412892396
25	18.500144412152284	26.928443778543965	34.77119699622723	19.80021481307652
26	18.089066194920303	32.39882123580699	30.16381753524559	19.348295034027114
27	18.230093687383793	33.02038016499088	29.848096467317724	18.901429680307597
28	17.16297813961045	30.185885517266275	34.80161380580176	17.849522537321516
29	17.36705056230482	26.941801902630107	34.70133761756052	20.98980991750456
30	18.830487210498763	31.09392205354081	31.29862627940141	18.77696445655902
31	23.95608065418705	28.241375886780872	28.878098091954442	18.924445367077638
32	25.753876563712836	27.690533783417877	28.67957651136343	17.876013141505858
33	25.40990486849468	28.155540913767897	27.476578154051662	18.95797606368576
34	20.390725129519648	28.86401790710688	28.836353954185245	21.908903009188226
35	19.84498258028413	27.78151343935592	31.05438922685344	21.319114753506508
36	26.02275393974403	27.891086159900357	27.38076971677167	18.705390183583948
37	19.9150224741412	31.944690145675757	29.53372926331751	18.606558116865536
38	20.05790024730581	32.41754968680615	27.51859306460639	20.005957001281658
39	19.891826272180804	31.966848385291623	28.260329981767967	19.880995360759606
40	23.309655757531996	27.57933642616026	28.412007870462297	20.698999945845443
41	17.965323031933135	27.09528494322797	30.231239958842536	24.70815206599635
42	20.115439464230914	27.535651750094768	28.606512988067944	23.74239579760637
43	20.226275791107824	27.23211545751575	28.77312850876401	23.768480242612416
44	19.512112569272706	29.485802480278718	30.47213748036897	20.529947470079605
45	18.724976081737278	32.56706139321624	27.746674007617738	20.96128851742874
46	20.576430131595576	31.78561113417694	27.853042583533405	19.78491615069408
47	20.740698954817045	28.17359243280322	28.844883296929435	22.240825315450294
48	20.98362727223496	26.47593732512591	31.98593786667148	20.554497535967652
49	20.70098561293933	26.416231925916566	32.25413831073885	20.628644150405258
50	19.702420708702636	30.99265303175262	29.60065527014098	19.704270989403756
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2352.0
1	2992.5
2	3633.0
3	10200.0
4	16767.0
5	13385.0
6	10003.0
7	10671.5
8	11340.0
9	12104.0
10	12868.0
11	13216.5
12	13565.0
13	13621.5
14	13678.0
15	13818.5
16	13959.0
17	13974.5
18	13990.0
19	13874.5
20	13759.0
21	15212.5
22	16666.0
23	17542.5
24	18419.0
25	23101.0
26	27783.0
27	31751.5
28	35720.0
29	42424.5
30	49129.0
31	57657.5
32	66186.0
33	74247.5
34	82309.0
35	89602.5
36	96896.0
37	104084.5
38	111273.0
39	117149.0
40	123025.0
41	147274.0
42	171523.0
43	182585.0
44	193647.0
45	192907.0
46	192167.0
47	186073.5
48	179980.0
49	166785.5
50	153591.0
51	137413.5
52	121236.0
53	109560.5
54	97885.0
55	86901.0
56	75917.0
57	70126.5
58	64336.0
59	63025.0
60	61714.0
61	55940.5
62	50167.0
63	42562.0
64	34957.0
65	28974.5
66	22992.0
67	18956.5
68	14921.0
69	12490.5
70	10060.0
71	8225.0
72	6390.0
73	5407.0
74	4424.0
75	3715.0
76	3006.0
77	2458.0
78	1910.0
79	1505.5
80	1101.0
81	720.0
82	339.0
83	235.0
84	131.0
85	126.0
86	121.0
87	71.5
88	22.0
89	21.5
90	21.0
91	11.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2215880.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.98879311514698
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.29529706789322	16.853013782772788
2	6.108846891825022	2.564346471692481
3	2.185933463387202	1.3764031567953205
4	1.2079197495672644	1.0141111089346984
5	0.8935766484904686	0.9377547703846429
6	0.6583337770560174	0.8290578868405224
7	0.5442059413574439	0.7995558140629639
8	0.44743314613041657	0.7512865389592511
9	0.3792650972939336	0.7164284996608616
>10	4.932410797466356	24.305545043424594
>50	1.5391837080419337	23.04755786350998
>100	0.8043249838878055	25.91273248231151
>500	0.002614982082332713	0.35474210486105123
>1k	4.3583034705545215E-4	0.17169956957818283
>5k	2.1791517352772607E-4	0.36576490621116897
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7997	0.36089499431377153	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01764535985703197	0.0
2	0.0	0.0	0.0	0.0761774103290792	0.0
3	0.0	0.0	0.0	0.1224795566546925	0.0
4	0.0	0.0	0.0	0.2441467949527953	0.0
5	0.0	0.0	0.0	0.4044894127840858	0.0
6	0.0	0.0	0.0	0.786549813166778	0.0
7	0.0	0.0	0.0	0.9591674639420907	0.0
8	0.0	0.0	0.0	1.4516129032258065	0.0
9	0.0	0.0	0.0	1.7119609365128075	0.0
10	0.0	0.0	0.0	2.092577215372674	0.0
11	0.0	0.0	0.0	2.369352130981822	0.0
12	0.0	0.0	0.0	2.5957633084824088	0.0
13	0.0	0.0	0.0	2.723342419264581	0.0
14	0.0	0.0	0.0	2.7819195985342167	0.0
15	0.0	0.0	0.0	2.8335469429752513	0.0
16	0.0	0.0	0.0	2.9270989403758327	0.0
17	0.0	0.0	0.0	3.0295864396989005	0.0
18	0.0	0.0	0.0	3.1806776540245862	0.0
19	0.0	0.0	0.0	3.25243244219001	0.0
20	0.0	0.0	0.0	3.3314980955647417	0.0
21	0.0	0.0	0.0	3.4281639799989168	0.0
22	0.0	0.0	0.0	3.530696608119573	0.0
23	0.0	0.0	0.0	3.6491597017889057	0.0
24	0.0	0.0	0.0	3.7383793346210084	0.0
25	0.0	0.0	0.0	3.8184378215426826	0.0
26	0.0	0.0	0.0	3.8983609220715922	0.0
27	0.0	0.0	0.0	3.9806758488726826	0.0
28	0.0	0.0	0.0	4.062674874090654	0.0
29	0.0	0.0	0.0	4.154737621170821	0.0
30	0.0	0.0	0.0	4.277623337003809	0.0
31	0.0	0.0	0.0	4.384668844883297	0.0
32	0.0	0.0	0.0	4.487968662562954	0.0
33	0.0	0.0	0.0	4.591494124230554	0.0
34	0.0	0.0	0.0	4.704270989403758	0.0
35	0.0	0.0	0.0	4.836453237539939	0.0
36	0.0	0.0	0.0	4.959654854956044	0.0
37	0.0	0.0	0.0	5.0893098904272795	0.0
38	0.0	0.0	0.0	5.218333122732278	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGAAA	20	7.8580633E-4	44.000004	26
GCGCGTT	25	4.444182E-5	44.0	18
AGACGCG	25	4.444182E-5	44.0	1
CGGATTA	25	4.444182E-5	44.0	6
AGTCGAA	30	2.5287845E-6	44.0	24
CGTTTTT	8205	0.0	40.916515	1
TATTACG	55	7.8216544E-11	40.000004	1
CGGTCTA	55	7.8216544E-11	40.000004	31
TCGGTAC	55	7.8216544E-11	40.000004	1
CGACAGG	200	0.0	39.600002	2
GCGATAA	290	0.0	39.448277	9
CATACGA	490	0.0	39.061226	18
CGCAATC	175	0.0	38.97143	20
CGAACGG	215	0.0	38.88372	2
TCCGACC	40	4.1268504E-7	38.500004	29
ACTTACG	40	4.1268504E-7	38.500004	1
CGTAATC	40	4.1268504E-7	38.500004	31
GGCGATA	1090	0.0	38.14679	8
CGTTAGG	360	0.0	37.88889	2
TAAGGGA	3175	0.0	37.76378	4
>>END_MODULE