FastQCFastQC Report
Thu 26 May 2016
SRR1546643_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546643_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2996505
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115800.38645021449989236No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA33160.11066225486024552No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT109650.041.271321
GCGATAT3000.040.3333329
AGCGTAA404.127487E-738.50000431
CGTTCGA800.038.50000414
CATACGA4650.038.32258218
AGGGCGA29400.038.0884366
TCGATAG1750.037.7142871
GGCGATA13500.037.6444448
ACGGGAT18650.037.39415
TAGGGCG12050.037.2448165
GGGCGAT59900.037.242077
TAAGGGA50150.037.1126634
TACGGGA13600.036.882354
TAGGGAT69750.036.8401455
TAATACG1500.036.6666681
TATCGTT301.3015945E-436.66666811
AGGGATT73000.036.4356166
ACATACG4950.036.017
GGCGATT19350.035.7002568
AGGGATC42650.035.5404436
Produced by FastQC (version 0.11.5)