##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546643_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2996505
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.92995206081752	31.0	31.0	33.0	30.0	34.0
2	31.303464202462536	31.0	31.0	34.0	30.0	34.0
3	31.459586751899295	31.0	31.0	34.0	30.0	34.0
4	35.240704420650054	37.0	35.0	37.0	33.0	37.0
5	35.024183507119126	35.0	35.0	37.0	32.0	37.0
6	35.105792247968886	37.0	35.0	37.0	32.0	37.0
7	35.67301806604694	37.0	35.0	37.0	35.0	37.0
8	35.7149856249197	37.0	35.0	37.0	35.0	37.0
9	37.47619376573709	39.0	37.0	39.0	35.0	39.0
10	36.670351960033436	39.0	35.0	39.0	32.0	39.0
11	36.28827350530034	38.0	35.0	39.0	31.0	39.0
12	35.874401677954815	37.0	35.0	39.0	31.0	39.0
13	35.75038753481139	37.0	35.0	39.0	30.0	39.0
14	36.69156600773234	38.0	35.0	40.0	31.0	41.0
15	36.85863130547087	38.0	35.0	40.0	31.0	41.0
16	36.88637229038496	38.0	35.0	40.0	31.0	41.0
17	36.78798500252795	38.0	35.0	40.0	31.0	41.0
18	36.70520089237295	38.0	35.0	40.0	31.0	41.0
19	36.685021049522696	38.0	35.0	40.0	31.0	41.0
20	36.565787809464695	38.0	35.0	40.0	31.0	41.0
21	36.45635665550366	38.0	35.0	40.0	30.0	41.0
22	36.38347541552575	38.0	35.0	40.0	30.0	41.0
23	36.17595799105958	38.0	34.0	40.0	30.0	41.0
24	36.12852272897926	38.0	34.0	40.0	30.0	41.0
25	35.99114234750151	38.0	34.0	40.0	30.0	41.0
26	35.860329951059654	38.0	34.0	40.0	29.0	41.0
27	35.68462024925705	38.0	34.0	40.0	29.0	41.0
28	35.614678099986484	38.0	34.0	40.0	29.0	41.0
29	35.50595410319689	38.0	34.0	40.0	28.0	41.0
30	35.48519758852397	38.0	34.0	40.0	27.0	41.0
31	35.44204865334782	38.0	34.0	40.0	27.0	41.0
32	35.262784477249326	38.0	34.0	40.0	27.0	41.0
33	35.070449740614485	38.0	34.0	40.0	26.0	41.0
34	34.987429021476686	38.0	34.0	40.0	25.0	41.0
35	34.78606676778447	38.0	33.0	40.0	25.0	41.0
36	34.610159836209185	37.0	33.0	40.0	24.0	41.0
37	34.68211499730519	38.0	33.0	40.0	24.0	41.0
38	34.63630696428005	37.0	33.0	40.0	24.0	41.0
39	34.54046697736196	37.0	33.0	40.0	24.0	41.0
40	34.532391569511816	37.0	33.0	40.0	23.0	41.0
41	34.55139237211351	37.0	33.0	40.0	24.0	41.0
42	34.295890712680276	37.0	33.0	40.0	23.0	41.0
43	34.35081770262356	37.0	33.0	40.0	23.0	41.0
44	34.1466798820626	37.0	33.0	40.0	23.0	41.0
45	33.98251563070978	37.0	33.0	40.0	23.0	41.0
46	34.070689353096355	37.0	33.0	40.0	23.0	41.0
47	33.91891420171166	37.0	33.0	40.0	23.0	41.0
48	33.970835690245806	36.0	33.0	40.0	23.0	41.0
49	33.88462125042341	36.0	33.0	40.0	23.0	41.0
50	33.37941935688411	36.0	32.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	4.0
13	5.0
14	21.0
15	101.0
16	279.0
17	790.0
18	1836.0
19	3534.0
20	6265.0
21	9559.0
22	14413.0
23	20622.0
24	29087.0
25	39815.0
26	51516.0
27	61383.0
28	70958.0
29	81380.0
30	96195.0
31	115477.0
32	139270.0
33	171240.0
34	239649.0
35	299207.0
36	252587.0
37	309453.0
38	416651.0
39	565205.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.74080970997879	24.155240855596773	29.340882127678746	13.763067306745691
2	30.272400680125678	26.030625678915936	30.127131441462634	13.569842199495746
3	27.834894318547775	26.212871328430953	32.54317947075009	13.40905488227118
4	24.59178276024902	29.59654664350635	31.61339627332509	14.198274322919534
5	21.83850852910307	33.47993746047479	30.73507302674282	13.946480983679319
6	21.468210465191948	42.06854318614519	26.909849975221135	9.553396373441727
7	89.72789966978196	3.1553426408432492	5.9468614268956665	1.1698962624791216
8	90.10370414866654	2.581307222914696	5.659293076434046	1.6556955519847287
9	84.6066667667833	5.358208980128516	7.499303355075329	2.535820898012852
10	37.64445579099651	38.394195904895874	13.102097276660643	10.859251027446975
11	28.923162150572086	25.971089652778822	30.13454007251782	14.97120812413128
12	28.536545075012388	24.08162175601242	30.228482849185966	17.153350319789222
13	22.124975596570003	29.591907906043875	31.747986404160848	16.535130093225277
14	18.554449266729073	32.355460778473585	31.182627761341962	17.907462193455377
15	17.11664088663293	26.49466628622345	39.23140458634309	17.157288240800533
16	20.58611615865817	24.58851228347692	37.44976230642031	17.375609251444597
17	20.446586940452292	24.494769740080528	30.95870021908857	24.099943100378606
18	21.580341097378447	25.03963784475581	34.02617382584044	19.35384723202531
19	24.65755939002271	26.122933217198035	30.13540775002878	19.08409964275047
20	26.37742970560703	26.491963137054668	28.8102639575105	18.3203431998278
21	23.31556263046449	28.461123875982185	30.223276784120166	18.000036709433157
22	22.72444064001228	25.749698398634408	29.34094887210267	22.184912089250645
23	20.6422815913873	29.19905022684761	29.176991194741873	20.981676987023214
24	19.830702768725565	26.554736267751935	35.363431731300295	18.251129232222205
25	19.781478756084173	27.14402278654633	33.131064356642156	19.943434100727348
26	19.109829618171837	31.252742778670484	29.784999524445983	19.852428078711696
27	19.36669553363001	30.972916781383645	30.17248427751664	19.4879034074697
28	18.249727599319872	29.004256625635534	34.09488721026663	18.651128564777967
29	18.91546985571524	26.2011576820329	34.06818944069842	20.815183021553445
30	20.159185451050472	28.68238164127876	31.757831206689126	19.400601700981642
31	23.408637729621677	28.061258032274267	29.248074006217244	19.282030231886814
32	24.228659721909356	27.294231112579485	29.681645784005035	18.79546338150612
33	23.62672513478202	27.925032663052455	28.670801483728543	19.777440718436978
34	20.353979052262552	27.83235803043879	30.111513246265233	21.70214967103342
35	19.880660970030085	27.471704535784188	31.68841700581177	20.959217488373955
36	24.1062504484391	27.28862458097016	29.001119637711266	19.60400533287947
37	20.602001331551257	30.630618003307188	29.989070600583013	18.778310064558543
38	20.82239141933686	29.826848278244157	28.52800178875056	20.822758513668425
39	20.98584851351825	29.781962653157596	29.104673611423976	20.12751522190018
40	22.685929107410132	27.579997363595254	28.970817669251343	20.763255859743268
41	19.960754278734726	26.810334039155613	29.59167430056015	23.637237381549507
42	21.004470207792078	27.210066394015698	28.404157510166012	23.381305888026215
43	20.721540594792934	27.36901823958245	29.424512890851172	22.484928274773445
44	19.875855371507807	28.613735001276485	30.619972267691857	20.890437359523844
45	19.25376396835647	31.05090764073479	28.243336820729482	21.45199157017926
46	20.72093989497765	30.291923424122437	28.76294216095084	20.224194519949073
47	20.175137368367484	28.401988316388593	29.412332033485676	22.010542281758248
48	20.821690602885695	27.657654500826794	31.002217583484757	20.51843731280275
49	21.025561445750967	26.90928264761781	31.312879504622888	20.75227640200834
50	20.04475213623872	30.10887684152037	29.59661338793027	20.24975763431064
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2056.0
1	2813.0
2	3570.0
3	13313.0
4	23056.0
5	17842.0
6	12628.0
7	13216.5
8	13805.0
9	14456.5
10	15108.0
11	15574.0
12	16040.0
13	15925.5
14	15811.0
15	15966.5
16	16122.0
17	16256.0
18	16390.0
19	16719.0
20	17048.0
21	18444.0
22	19840.0
23	21926.5
24	24013.0
25	28524.0
26	33035.0
27	42537.0
28	52039.0
29	59368.5
30	66698.0
31	78417.0
32	90136.0
33	100480.5
34	110825.0
35	123544.0
36	136263.0
37	147972.0
38	159681.0
39	170010.0
40	180339.0
41	200887.5
42	221436.0
43	237177.0
44	252918.0
45	253640.0
46	254362.0
47	246589.0
48	238816.0
49	223376.5
50	207937.0
51	187256.0
52	166575.0
53	148863.0
54	131151.0
55	119254.5
56	107358.0
57	99856.5
58	92355.0
59	85724.0
60	79093.0
61	74040.0
62	68987.0
63	59972.5
64	50958.0
65	42541.5
66	34125.0
67	28701.0
68	23277.0
69	19839.0
70	16401.0
71	13682.0
72	10963.0
73	8859.5
74	6756.0
75	5263.5
76	3771.0
77	3103.0
78	2435.0
79	1986.0
80	1537.0
81	983.5
82	430.0
83	320.0
84	210.0
85	134.5
86	59.0
87	47.0
88	35.0
89	23.5
90	12.0
91	18.5
92	25.0
93	22.0
94	19.0
95	9.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2996505.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.72361271347071
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.82954792878498	17.12462569582902
2	6.722575649645189	2.9207725969980167
3	2.424888904610111	1.5803204231082686
4	1.3750664115860245	1.1948564072238685
5	0.9321723686896053	1.0125075759805808
6	0.7327364575681908	0.9550609815151107
7	0.5981344670678219	0.9095549059211694
8	0.483313493841456	0.8399452127524961
9	0.41393000380186906	0.8092849583769537
>10	5.2949609532817705	26.833617660691655
>50	1.5078004981475146	23.145623649777537
>100	0.6815879393137758	21.702478835003227
>500	0.0028156488529595927	0.4183419049494003
>1k	3.128498725510658E-4	0.15950750563175087
>5k	0.0	0.0
>10k+	1.564249362755329E-4	0.3935016862410897
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11580	0.38645021449989236	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3316	0.11066225486024552	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.3372211960267045E-5	0.0	0.0	0.03624222218885001	0.0
2	3.3372211960267045E-5	0.0	0.0	0.1240445118563126	0.0
3	3.3372211960267045E-5	0.0	0.0	0.1840143767489125	0.0
4	3.3372211960267045E-5	0.0	0.0	0.3029863123872645	0.0
5	3.3372211960267045E-5	0.0	0.0	0.491873032082376	0.0
6	3.3372211960267045E-5	0.0	0.0	0.8529603654924653	0.0
7	3.3372211960267045E-5	0.0	0.0	1.0430151126061862	0.0
8	3.3372211960267045E-5	0.0	0.0	1.5784388812967107	0.0
9	3.3372211960267045E-5	0.0	0.0	1.8304991982326078	0.0
10	3.3372211960267045E-5	3.3372211960267045E-5	0.0	2.211576486606897	0.0
11	3.3372211960267045E-5	3.3372211960267045E-5	0.0	2.4877982850020275	0.0
12	3.3372211960267045E-5	3.3372211960267045E-5	0.0	2.737789524796388	0.0
13	3.3372211960267045E-5	3.3372211960267045E-5	0.0	2.868308245772992	0.0
14	3.3372211960267045E-5	3.3372211960267045E-5	0.0	2.936387558171937	0.0
15	3.3372211960267045E-5	3.3372211960267045E-5	0.0	2.9995611554127226	0.0
16	3.3372211960267045E-5	3.3372211960267045E-5	0.0	3.1160635473660148	0.0
17	3.3372211960267045E-5	3.3372211960267045E-5	0.0	3.2483509955765135	0.0
18	3.3372211960267045E-5	3.3372211960267045E-5	0.0	3.434033982923439	0.0
19	3.3372211960267045E-5	3.3372211960267045E-5	0.0	3.5326822414779886	0.0
20	3.3372211960267045E-5	3.3372211960267045E-5	0.0	3.6351349321960083	3.3372211960267045E-5
21	3.3372211960267045E-5	3.3372211960267045E-5	0.0	3.766654819531421	3.3372211960267045E-5
22	3.3372211960267045E-5	3.3372211960267045E-5	0.0	3.9085534647864764	3.3372211960267045E-5
23	3.3372211960267045E-5	3.3372211960267045E-5	0.0	4.06283320067879	3.3372211960267045E-5
24	3.3372211960267045E-5	3.3372211960267045E-5	0.0	4.19021493373113	3.3372211960267045E-5
25	3.3372211960267045E-5	3.3372211960267045E-5	0.0	4.305015342874449	3.3372211960267045E-5
26	3.3372211960267045E-5	3.3372211960267045E-5	0.0	4.414476198104125	3.3372211960267045E-5
27	3.3372211960267045E-5	3.3372211960267045E-5	0.0	4.529677073790967	3.3372211960267045E-5
28	3.3372211960267045E-5	3.3372211960267045E-5	0.0	4.646813537771504	3.3372211960267045E-5
29	3.3372211960267045E-5	3.3372211960267045E-5	0.0	4.782338090542148	3.3372211960267045E-5
30	3.3372211960267045E-5	3.3372211960267045E-5	0.0	4.95997837480665	3.3372211960267045E-5
31	3.3372211960267045E-5	3.3372211960267045E-5	0.0	5.115926721296978	3.3372211960267045E-5
32	3.3372211960267045E-5	3.3372211960267045E-5	0.0	5.269138546406563	3.3372211960267045E-5
33	3.3372211960267045E-5	3.3372211960267045E-5	0.0	5.421315832945381	3.3372211960267045E-5
34	3.3372211960267045E-5	3.3372211960267045E-5	0.0	5.578799301185882	3.3372211960267045E-5
35	3.3372211960267045E-5	3.3372211960267045E-5	0.0	5.7630139112065555	3.3372211960267045E-5
36	3.3372211960267045E-5	3.3372211960267045E-5	0.0	5.9259370499965796	3.3372211960267045E-5
37	3.3372211960267045E-5	3.3372211960267045E-5	0.0	6.100006507581332	3.3372211960267045E-5
38	3.3372211960267045E-5	3.3372211960267045E-5	0.0	6.282819484699675	3.3372211960267045E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	10965	0.0	41.27132	1
GCGATAT	300	0.0	40.333332	9
AGCGTAA	40	4.127487E-7	38.500004	31
CGTTCGA	80	0.0	38.500004	14
CATACGA	465	0.0	38.322582	18
AGGGCGA	2940	0.0	38.088436	6
TCGATAG	175	0.0	37.714287	1
GGCGATA	1350	0.0	37.644444	8
ACGGGAT	1865	0.0	37.3941	5
TAGGGCG	1205	0.0	37.244816	5
GGGCGAT	5990	0.0	37.24207	7
TAAGGGA	5015	0.0	37.112663	4
TACGGGA	1360	0.0	36.88235	4
TAGGGAT	6975	0.0	36.840145	5
TAATACG	150	0.0	36.666668	1
TATCGTT	30	1.3015945E-4	36.666668	11
AGGGATT	7300	0.0	36.435616	6
ACATACG	495	0.0	36.0	17
GGCGATT	1935	0.0	35.700256	8
AGGGATC	4265	0.0	35.540443	6
>>END_MODULE