FastQCFastQC Report
Thu 26 May 2016
SRR1546642_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546642_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3177635
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193520.6090063836784275No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG254.44462E-544.038
CGTTTTT214550.042.7182461
TTTCGCG650.040.6153871
CGTAAGG6600.039.02
AGGGCGA32600.037.8588946
TAATCCG357.2942657E-637.7142871
AACGGGA15050.037.7142834
CGAGGGA25050.037.5014
GGACCGA17900.037.486038
ATCGACG1000.037.3999981
TAGGGAC38700.037.235145
GGGCGAT65800.037.1458977
TCGTAAG3100.036.9032251
TAGGGAT87150.036.8559955
GCGATCT4300.036.8372089
AAGGGAT88000.036.755
TCGATAG1500.036.6666681
ACGTCGT301.3016124E-436.66666438
GACCGAT17650.036.6458939
GGCGATC10450.036.631588
Produced by FastQC (version 0.11.5)