##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546642_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3177635
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.056594605736656	31.0	31.0	33.0	30.0	34.0
2	31.45146657813122	31.0	31.0	34.0	30.0	34.0
3	31.56105688664683	31.0	31.0	34.0	30.0	34.0
4	35.34553905656251	37.0	35.0	37.0	33.0	37.0
5	35.160232688776404	37.0	35.0	37.0	32.0	37.0
6	35.24039765423027	37.0	35.0	37.0	32.0	37.0
7	35.78176631362633	37.0	35.0	37.0	35.0	37.0
8	35.8324445696249	37.0	35.0	37.0	35.0	37.0
9	37.61018933892659	39.0	37.0	39.0	35.0	39.0
10	36.87789409419269	39.0	37.0	39.0	32.0	39.0
11	36.47994750813105	38.0	35.0	39.0	32.0	39.0
12	36.22163212577908	38.0	35.0	39.0	31.0	39.0
13	36.16365504534033	38.0	35.0	39.0	32.0	39.0
14	37.184039387783685	39.0	36.0	41.0	31.0	41.0
15	37.308435676218316	39.0	36.0	41.0	32.0	41.0
16	37.30026985478194	39.0	36.0	41.0	32.0	41.0
17	37.20253584820157	39.0	35.0	41.0	32.0	41.0
18	37.09342356815682	39.0	36.0	40.0	32.0	41.0
19	37.02217277944131	38.0	35.0	40.0	31.0	41.0
20	36.86246910044734	38.0	35.0	40.0	31.0	41.0
21	36.785979824617996	38.0	35.0	40.0	31.0	41.0
22	36.71717960055198	38.0	35.0	40.0	31.0	41.0
23	36.513321385244055	38.0	35.0	40.0	30.0	41.0
24	36.4694283641765	38.0	35.0	40.0	30.0	41.0
25	36.334758082662105	38.0	35.0	40.0	30.0	41.0
26	36.25016372239102	38.0	35.0	40.0	30.0	41.0
27	36.06781143838106	38.0	35.0	40.0	30.0	41.0
28	36.030120828855424	38.0	35.0	40.0	30.0	41.0
29	35.90482607347918	38.0	34.0	40.0	30.0	41.0
30	35.83957943564947	38.0	34.0	40.0	29.0	41.0
31	35.72725533297562	38.0	34.0	40.0	29.0	41.0
32	35.476520745774764	38.0	34.0	40.0	27.0	41.0
33	35.17329932481232	38.0	34.0	40.0	25.0	41.0
34	34.998677632893646	38.0	34.0	40.0	24.0	41.0
35	34.75175374138313	38.0	34.0	40.0	24.0	41.0
36	34.5793585481026	38.0	33.0	40.0	23.0	41.0
37	34.65567473923217	38.0	33.0	40.0	23.0	41.0
38	34.60846415651892	38.0	33.0	40.0	23.0	41.0
39	34.50450287713976	38.0	33.0	40.0	23.0	41.0
40	34.53079759003158	38.0	33.0	40.0	23.0	41.0
41	34.54479730994907	38.0	33.0	40.0	23.0	41.0
42	34.3139079850266	38.0	33.0	40.0	22.0	41.0
43	34.38271292958442	38.0	33.0	40.0	22.0	41.0
44	34.19273453370195	38.0	33.0	40.0	21.0	41.0
45	34.03051986776329	37.0	33.0	40.0	20.0	41.0
46	34.11123587196138	37.0	33.0	40.0	21.0	41.0
47	33.995234191466295	37.0	33.0	40.0	20.0	41.0
48	34.036738014277915	37.0	33.0	40.0	21.0	41.0
49	33.94774100864322	37.0	33.0	40.0	20.0	41.0
50	33.502284875386884	37.0	32.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	4.0
13	4.0
14	12.0
15	63.0
16	229.0
17	650.0
18	1637.0
19	3352.0
20	5973.0
21	9491.0
22	14525.0
23	21689.0
24	31425.0
25	45193.0
26	60994.0
27	69744.0
28	73414.0
29	79088.0
30	91163.0
31	108707.0
32	132583.0
33	163676.0
34	227955.0
35	288979.0
36	266055.0
37	330306.0
38	467126.0
39	683595.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.282941558737868	24.995035616110727	29.542600078360167	16.179422746791243
2	32.62898350502811	25.842521246146898	28.488388376890363	13.040106871934631
3	26.388619208939986	25.478099278236805	34.92625805040541	13.207023462417805
4	22.927051093029878	28.124627277833987	35.02611218720841	13.922209441927723
5	21.34895921023025	32.67351347779087	32.99003818877876	12.987489123200117
6	19.957012054562593	41.24995476195347	29.08864611574331	9.704387067740631
7	88.14974029427546	2.6436327646189697	8.313321070544603	0.8933058705609676
8	89.21458883729566	1.9040890473575476	7.870413058768549	1.0109090565782413
9	83.3833967714983	5.553784496960789	9.243509717132396	1.8193090144085144
10	33.27729585053035	43.91410593098326	14.296198273244096	8.512399945242295
11	22.978819153238177	25.32119013039572	36.742388600326976	14.957602116039128
12	24.703560981673476	24.56377148413836	34.349791590286486	16.382875943901677
13	20.268186874829865	28.765827415672344	35.45145996944268	15.514525740055104
14	17.852113285509503	31.171169753606065	33.664030009739946	17.31268695114448
15	16.52449069827088	26.979467434113737	39.684041748029586	16.812000119585793
16	19.12815663221232	25.44486701587816	38.5034152758262	16.923561076083313
17	19.402291326725692	24.71105082868234	34.42937908224199	21.457278762349986
18	19.981243912532435	25.446660802766836	36.0317972328477	18.54029805185303
19	22.47463915773838	26.113760705682054	32.79973942885196	18.611860707727605
20	23.78394623674525	26.400074269071176	32.67149940128429	17.14448009289928
21	21.755676784778615	27.688579714158486	33.443740391832286	17.112003109230606
22	21.511344128573608	25.531283485988794	32.822366319605614	20.135006065831977
23	18.60172738530385	28.690771595856667	33.36336615124141	19.344134867598072
24	17.69639999559421	26.795903242505826	38.182547712371	17.325149049528974
25	18.36708747228678	28.207015594931452	35.6286987020221	17.79719823075967
26	18.927409850407614	30.354367320349883	32.29873789783911	18.41948493140339
27	17.759560176042875	30.263859757335247	33.92639494466797	18.050185121953906
28	17.27114662319618	28.803654290061637	36.235753949084774	17.68944513765741
29	18.032404602794216	26.13556937785491	36.20261609656238	19.629409922788486
30	18.788753270907453	28.970979989835204	34.134348343972796	18.105918395284544
31	21.049050630421682	28.50780533321165	32.55650822073649	17.88663581563018
32	21.981756872642706	28.244181600467016	32.26541122564423	17.508650301246053
33	21.502532543857303	28.836477443129876	31.392906989002828	18.268083024009997
34	19.29932166532657	29.11762993547088	31.496191349856105	20.08685704934645
35	18.990664440692527	29.273406165277006	32.14868919809859	19.58724019593188
36	22.397852490924855	29.225918017645196	30.064057073893007	18.312172417536942
37	19.585918458224434	31.101935873692227	31.342995655574036	17.969150012509303
38	19.873270529812263	30.779903922256647	30.55347766499299	18.793347882938097
39	19.76085988478853	31.281629262014043	30.479240063758112	18.478270789439318
40	21.723955079799914	28.97359199530468	29.92766633046275	19.374786594432653
41	18.854651336607255	28.378495327499852	31.1959995405388	21.570853795354093
42	19.697825584121524	28.95760526303367	30.014649259590858	21.329919893253944
43	19.568389698628067	28.917071973338665	30.74361907519271	20.770919252840557
44	19.43064574754495	30.198024631526277	30.744909342954745	19.626420277974027
45	18.638389871712768	32.0830428919621	29.568814542891175	19.709752693433956
46	19.823201846656396	31.399798907048798	29.84512066363821	18.931878582656598
47	19.56864145819139	29.512703630215555	30.512377916280503	20.406276995312552
48	19.679321256217282	28.78766755779062	31.92191677143536	19.61109441455674
49	19.883340912345187	28.879308038840207	31.913356946282377	19.32399410253223
50	19.465955026301007	30.675109004023433	30.825692692836025	19.03324327683954
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1851.0
1	2507.5
2	3164.0
3	21518.5
4	39873.0
5	31443.5
6	23014.0
7	23512.5
8	24011.0
9	24525.5
10	25040.0
11	25525.5
12	26011.0
13	25597.0
14	25183.0
15	24461.5
16	23740.0
17	23979.5
18	24219.0
19	23779.5
20	23340.0
21	24902.0
22	26464.0
23	30129.0
24	33794.0
25	40276.0
26	46758.0
27	55173.0
28	63588.0
29	76940.5
30	90293.0
31	98855.0
32	107417.0
33	123626.0
34	139835.0
35	153153.0
36	166471.0
37	175252.5
38	184034.0
39	199328.0
40	214622.0
41	230075.5
42	245529.0
43	258314.0
44	271099.0
45	261550.0
46	252001.0
47	246239.0
48	240477.0
49	226428.5
50	212380.0
51	190791.5
52	169203.0
53	147983.5
54	126764.0
55	111364.0
56	95964.0
57	85226.5
58	74489.0
59	68135.0
60	61781.0
61	53565.5
62	45350.0
63	36900.5
64	28451.0
65	22494.0
66	16537.0
67	13012.5
68	9488.0
69	8112.5
70	6737.0
71	5181.5
72	3626.0
73	2881.5
74	2137.0
75	1857.0
76	1577.0
77	1161.0
78	745.0
79	547.0
80	349.0
81	239.0
82	129.0
83	86.0
84	43.0
85	40.5
86	38.0
87	25.5
88	13.0
89	9.0
90	5.0
91	2.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3177635.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.923673638227406
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.17042864211115	16.91859292071761
2	6.36745579329236	2.791960454359642
3	2.618227098307289	1.7220346924215646
4	1.637345245672484	1.4358649119690723
5	1.1472882187315987	1.2576386238227413
6	0.8925305282229375	1.1740528807748638
7	0.7692436470106091	1.1805252665738277
8	0.6214863224321707	1.0900210642900063
9	0.5081038873667947	1.0025553420849835
>10	6.260894818416224	31.40569825659315
>50	1.4624294770618749	22.423542219783933
>100	0.5429591445046533	16.69834255433682
>500	0.0011688559052717067	0.16192185729163722
>1k	2.922139763179266E-4	0.11736531851959617
>5k	0.0	0.0
>10k+	1.461069881589633E-4	0.6198836364604872
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19352	0.6090063836784275	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.016238491834335914	0.0
2	0.0	0.0	0.0	0.06838419138762004	0.0
3	0.0	0.0	0.0	0.11968020241468891	0.0
4	0.0	0.0	0.0	0.18476004953369407	0.0
5	0.0	0.0	0.0	0.2987441918281993	0.0
6	0.0	0.0	0.0	0.5263033671268097	0.0
7	0.0	0.0	0.0	0.6431827443995298	0.0
8	0.0	0.0	0.0	0.9222896902885322	0.0
9	0.0	0.0	0.0	1.0859648763939218	0.0
10	0.0	0.0	0.0	1.3225559260267463	0.0
11	0.0	0.0	0.0	1.4989449700799493	0.0
12	0.0	0.0	0.0	1.658969642517155	0.0
13	0.0	0.0	0.0	1.7592643585559702	0.0
14	0.0	0.0	0.0	1.8175466974652532	0.0
15	0.0	0.0	0.0	1.8644683860795843	0.0
16	0.0	0.0	0.0	1.9424509108188952	0.0
17	0.0	0.0	0.0	2.0327696541610347	0.0
18	0.0	0.0	0.0	2.15131693854077	0.0
19	0.0	0.0	0.0	2.2159247364785446	0.0
20	0.0	0.0	0.0	2.2867006437177335	0.0
21	0.0	0.0	0.0	2.370536578304305	0.0
22	0.0	0.0	0.0	2.4649149446050287	0.0
23	3.146994541537968E-5	0.0	0.0	2.5738324256876575	0.0
24	3.146994541537968E-5	0.0	0.0	2.6632700105581666	0.0
25	3.146994541537968E-5	0.0	0.0	2.7428260325682463	0.0
26	3.146994541537968E-5	0.0	0.0	2.8204309179625726	0.0
27	3.146994541537968E-5	0.0	0.0	2.901088388062191	0.0
28	3.146994541537968E-5	0.0	0.0	2.988952475661931	0.0
29	3.146994541537968E-5	0.0	0.0	3.0860372572683774	0.0
30	3.146994541537968E-5	0.0	0.0	3.2098085525870657	0.0
31	3.146994541537968E-5	0.0	0.0	3.3188833833967717	0.0
32	3.146994541537968E-5	0.0	0.0	3.421978924577555	0.0
33	3.146994541537968E-5	0.0	0.0	3.5226198100159394	0.0
34	3.146994541537968E-5	0.0	0.0	3.634243706404291	0.0
35	3.146994541537968E-5	0.0	0.0	3.7597458487208253	0.0
36	3.146994541537968E-5	0.0	0.0	3.8791113516813605	0.0
37	3.146994541537968E-5	0.0	0.0	4.002851177054634	0.0
38	3.146994541537968E-5	0.0	0.0	4.13269617183849	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACACCG	25	4.44462E-5	44.0	38
CGTTTTT	21455	0.0	42.718246	1
TTTCGCG	65	0.0	40.615387	1
CGTAAGG	660	0.0	39.0	2
AGGGCGA	3260	0.0	37.858894	6
TAATCCG	35	7.2942657E-6	37.714287	1
AACGGGA	1505	0.0	37.714283	4
CGAGGGA	2505	0.0	37.501	4
GGACCGA	1790	0.0	37.48603	8
ATCGACG	100	0.0	37.399998	1
TAGGGAC	3870	0.0	37.23514	5
GGGCGAT	6580	0.0	37.145897	7
TCGTAAG	310	0.0	36.903225	1
TAGGGAT	8715	0.0	36.855995	5
GCGATCT	430	0.0	36.837208	9
AAGGGAT	8800	0.0	36.75	5
TCGATAG	150	0.0	36.666668	1
ACGTCGT	30	1.3016124E-4	36.666664	38
GACCGAT	1765	0.0	36.645893	9
GGCGATC	1045	0.0	36.63158	8
>>END_MODULE