FastQCFastQC Report
Thu 26 May 2016
SRR1546641_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546641_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3332845
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115860.34763092793094186No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGT207.858638E-444.037
CGGATTA351.447188E-744.012
CGTCGGA207.858638E-444.016
CGTTTTT112250.041.8636971
TACGACG950.039.368421
TTACGCG900.039.111111
TACGGGA16250.038.724
GCGATAT6250.038.7199979
ACGGGAC11350.038.5726855
TCGTAAG1950.038.3589741
TAGGGAC38800.038.1030925
TACGCGG2450.037.7142872
CGGTCTA1400.037.71428731
TCGAACG357.294373E-637.7142871
GCGATGT6900.037.623199
AACGGGA14350.037.5609744
GTTACGG3250.037.230772
CGACGGT1300.037.23076628
ACGATTG1300.037.2307661
ACTACGG1600.037.1252
Produced by FastQC (version 0.11.5)