##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546641_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3332845
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.986982892993822	31.0	31.0	33.0	30.0	34.0
2	31.36104229269588	31.0	31.0	34.0	30.0	34.0
3	31.514148722787887	31.0	31.0	34.0	30.0	34.0
4	35.285237387277235	37.0	35.0	37.0	33.0	37.0
5	35.07938172942336	37.0	35.0	37.0	32.0	37.0
6	35.15967739273803	37.0	35.0	37.0	32.0	37.0
7	35.7230300238985	37.0	35.0	37.0	35.0	37.0
8	35.76601402105408	37.0	35.0	37.0	35.0	37.0
9	37.526625450628515	39.0	37.0	39.0	35.0	39.0
10	36.74864777689932	39.0	37.0	39.0	32.0	39.0
11	36.341375311483134	38.0	35.0	39.0	32.0	39.0
12	35.94965322419735	37.0	35.0	39.0	31.0	39.0
13	35.83870627046862	37.0	35.0	39.0	30.0	39.0
14	36.79271433264973	39.0	35.0	41.0	31.0	41.0
15	36.964602614282995	39.0	35.0	41.0	31.0	41.0
16	36.9941410416626	38.0	35.0	41.0	31.0	41.0
17	36.91036726880488	38.0	35.0	40.0	31.0	41.0
18	36.84957596287856	38.0	35.0	40.0	31.0	41.0
19	36.83339729270338	38.0	35.0	40.0	31.0	41.0
20	36.70828136321971	38.0	35.0	40.0	31.0	41.0
21	36.612067467884046	38.0	35.0	40.0	31.0	41.0
22	36.541738064626465	38.0	35.0	40.0	30.0	41.0
23	36.3462150205005	38.0	35.0	40.0	30.0	41.0
24	36.316792710132034	38.0	35.0	40.0	30.0	41.0
25	36.17082462580768	38.0	34.0	40.0	30.0	41.0
26	36.07118872914882	38.0	34.0	40.0	30.0	41.0
27	35.87950744784111	38.0	34.0	40.0	29.0	41.0
28	35.84850690626177	38.0	34.0	40.0	29.0	41.0
29	35.75305332231172	38.0	34.0	40.0	29.0	41.0
30	35.713070364808445	38.0	34.0	40.0	29.0	41.0
31	35.643256137024075	38.0	34.0	40.0	29.0	41.0
32	35.46047085898084	38.0	34.0	40.0	27.0	41.0
33	35.25424494688472	38.0	34.0	40.0	27.0	41.0
34	35.19896514839424	38.0	34.0	40.0	26.0	41.0
35	34.99541502830164	38.0	34.0	40.0	25.0	41.0
36	34.815380253207096	38.0	33.0	40.0	24.0	41.0
37	34.89822899054712	38.0	33.0	40.0	25.0	41.0
38	34.85859678442892	38.0	33.0	40.0	25.0	41.0
39	34.76819144004597	38.0	33.0	40.0	24.0	41.0
40	34.799956793670276	38.0	33.0	40.0	24.0	41.0
41	34.84040901992142	38.0	34.0	40.0	24.0	41.0
42	34.59349984772769	37.0	33.0	40.0	24.0	41.0
43	34.67752115684948	37.0	33.0	40.0	24.0	41.0
44	34.4746170313951	37.0	33.0	40.0	23.0	41.0
45	34.2884247542265	37.0	33.0	40.0	23.0	41.0
46	34.36810892795795	37.0	33.0	40.0	23.0	41.0
47	34.23107645270032	37.0	33.0	40.0	23.0	41.0
48	34.298053764876556	37.0	33.0	40.0	23.0	41.0
49	34.20859775957178	37.0	33.0	40.0	23.0	41.0
50	33.7235004928222	36.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	5.0
14	18.0
15	90.0
16	246.0
17	672.0
18	1648.0
19	3321.0
20	5664.0
21	9142.0
22	13855.0
23	20181.0
24	29074.0
25	40726.0
26	53470.0
27	64125.0
28	73513.0
29	85517.0
30	101532.0
31	123557.0
32	151503.0
33	186689.0
34	265569.0
35	338559.0
36	277918.0
37	340973.0
38	471634.0
39	673641.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.758272586933984	24.272295891348083	30.30741003557021	13.66202148614772
2	29.841801823967213	26.59586629441213	30.150217006791497	13.412114874829161
3	27.617305935319525	26.223541748866207	32.70650150247011	13.452650813344155
4	24.00999746462857	29.43071159924929	32.14802968634905	14.41126124977309
5	21.99370207735433	33.55565590358987	30.711929297642104	13.738712721413687
6	21.214007852150342	42.53093078135947	26.433272474417503	9.82178889207269
7	90.46793355226541	2.963474148962823	5.525039418274777	1.043552880496993
8	90.89909671766914	2.4520192208158496	5.17971882880842	1.4691652327065916
9	85.0027228989047	5.745931779005624	6.959579578408237	2.291765743681449
10	37.31700694151694	40.856475473656886	12.214459418304783	9.612058166521395
11	27.275975930473816	25.64844749755839	32.26933745793759	14.806239114030205
12	28.040907993020976	24.22062832204918	31.099856128922887	16.638607556006953
13	20.937997416621535	31.724187593482444	31.711405720938117	15.626409268957902
14	17.71999597941098	33.787769908291565	31.178137597157985	17.314096515139468
15	16.70863781543996	26.84883335408637	39.777997476630325	16.664531353843337
16	18.8938579501897	24.880394977864256	38.796403673138116	17.429343398807926
17	18.742785818122353	24.730553026018313	32.17122308418183	24.3554380716775
18	20.152332316684394	25.53134034136001	34.74848065241558	19.567846689540016
19	23.865676321581113	26.64573359997239	30.482275653383223	19.006314425063273
20	25.603650934861953	26.754769573742553	29.659225076473707	17.982354414921787
21	22.14750460942528	28.687052653213698	31.299175329185726	17.8662674081753
22	22.26899240738768	26.611438575751343	29.01197025364216	22.10759876321881
23	18.94216502717648	30.007306070339308	29.89442953392672	21.156099368557495
24	18.259685043858926	27.196314260039095	36.402472962288975	18.141527733813003
25	18.245492964719332	28.328770164829148	33.99795069977752	19.427786170674004
26	18.1865043228833	32.370572288840314	30.393942712607398	19.048980675668986
27	18.351168446177365	32.265916956834175	30.650870352506644	18.732044244481816
28	17.430393552655463	30.207525402471465	34.591167606054285	17.770913438818788
29	18.032851812790575	27.01025700265089	34.59803861265676	20.358852571901785
30	19.209534196759826	30.545194871048608	31.35537356222687	18.8898973699647
31	22.960623731376646	28.88475161611176	29.329386755159632	18.82523789735196
32	24.467114432264328	27.769008159695396	29.8562639426676	17.907613465372677
33	23.2915422109339	28.958832468956704	29.093342174628585	18.656283145480813
34	19.91199710757626	28.596709417929727	30.35238662464051	21.1389068498535
35	19.342363656275644	27.827306700431613	31.973014046557818	20.85731559673492
36	23.616699846527517	28.381307861601723	29.127517181267056	18.874475110603704
37	19.69251495344068	31.867608604660585	29.576833006035386	18.863043435863354
38	19.348814601339097	31.80396928149974	29.360501313442423	19.486714803718744
39	19.74205221064886	31.345502116060004	29.32182564745735	19.590620025833786
40	21.841249743087364	28.44116663091143	29.56978197305905	20.147801652942157
41	18.659043549880057	27.56185781216948	29.906341278997374	23.872757358953088
42	19.637216852268857	28.279472942786114	28.91553612604246	23.16777407890256
43	19.59521069836731	28.719547413696105	29.486039704816758	22.199202183119827
44	19.535351929057608	29.876756944892424	30.507239310558997	20.080651815490967
45	18.29887078456994	33.090797801877976	28.495534595818288	20.114796817733797
46	20.061058945135464	31.68128130771158	28.73779008624763	19.51986966090532
47	19.397781775030044	28.939059572227332	29.951827942793617	21.71133070994901
48	20.12433821554858	27.823976212515134	32.177343980893205	19.874341591043088
49	19.835245863519006	27.9938310962556	31.827432718893316	20.343490321332077
50	19.476873361947526	30.94491342981747	29.841351758032552	19.736861450202454
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2625.0
1	3356.5
2	4088.0
3	13757.0
4	23426.0
5	18395.5
6	13365.0
7	14016.0
8	14667.0
9	15612.0
10	16557.0
11	17038.0
12	17519.0
13	17667.5
14	17816.0
15	17856.5
16	17897.0
17	17857.5
18	17818.0
19	18772.5
20	19727.0
21	21114.0
22	22501.0
23	24075.0
24	25649.0
25	34089.5
26	42530.0
27	51663.5
28	60797.0
29	75907.0
30	91017.0
31	102395.0
32	113773.0
33	126163.0
34	138553.0
35	153512.5
36	168472.0
37	175260.0
38	182048.0
39	201011.0
40	219974.0
41	249712.0
42	279450.0
43	292441.0
44	305432.0
45	301868.0
46	298304.0
47	286157.5
48	274011.0
49	252277.0
50	230543.0
51	201196.0
52	171849.0
53	153748.5
54	135648.0
55	119388.5
56	103129.0
57	92701.0
58	82273.0
59	75564.0
60	68855.0
61	59722.0
62	50589.0
63	43615.5
64	36642.0
65	30100.5
66	23559.0
67	19232.5
68	14906.0
69	12555.5
70	10205.0
71	8568.0
72	6931.0
73	5489.0
74	4047.0
75	3306.0
76	2565.0
77	2156.5
78	1748.0
79	1253.5
80	759.0
81	595.5
82	432.0
83	258.5
84	85.0
85	58.0
86	31.0
87	28.0
88	25.0
89	15.0
90	5.0
91	3.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3332845.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.110098464177366
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.45886928138647	15.979256851008799
2	7.127469301425496	2.866682189041364
3	2.3811284396266714	1.4365418213023613
4	1.332900106553433	1.0721900954280816
5	0.8783634839869938	0.8831988075158161
6	0.6680299877986182	0.8060489298992047
7	0.5057630134731507	0.7119660800338884
8	0.4301538040010028	0.6920348282560493
9	0.3680386380356587	0.6661163924566946
>10	4.435303988499289	20.87519410729585
>50	1.4092437613566953	20.347755371444478
>100	0.9995831958751422	32.52811275473969
>500	0.004395204160640107	0.5769906928171218
>1k	6.062350566400147E-4	0.2047858256319392
>5k	0.0	0.0
>10k+	1.5155876416000368E-4	0.3531252531286869
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11586	0.34763092793094186	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03798556488525569	0.0
2	0.0	0.0	0.0	0.14765163096393621	0.0
3	0.0	0.0	0.0	0.21360129258936433	0.0
4	0.0	0.0	0.0	0.34406040484931044	0.0
5	0.0	0.0	0.0	0.5569415919432197	0.0
6	0.0	0.0	0.0	0.9078129946037095	0.0
7	0.0	0.0	0.0	1.087419306928465	0.0
8	0.0	0.0	0.0	1.5905330130864173	0.0
9	0.0	0.0	0.0	1.8224969958098862	0.0
10	0.0	0.0	0.0	2.1857602138713323	0.0
11	0.0	0.0	0.0	2.478363080191248	0.0
12	0.0	0.0	0.0	2.734540610199394	0.0
13	3.000439564396184E-5	0.0	0.0	2.8669500081761976	0.0
14	3.000439564396184E-5	0.0	0.0	2.934639924748976	0.0
15	3.000439564396184E-5	0.0	0.0	3.0003195468136084	0.0
16	3.000439564396184E-5	0.0	0.0	3.126308004122604	0.0
17	3.000439564396184E-5	0.0	0.0	3.271799318600175	0.0
18	3.000439564396184E-5	0.0	0.0	3.4784395914001403	0.0
19	3.000439564396184E-5	0.0	0.0	3.5808145893373378	0.0
20	3.000439564396184E-5	0.0	0.0	3.6897905543162075	0.0
21	3.000439564396184E-5	0.0	0.0	3.8232201017449055	0.0
22	3.000439564396184E-5	0.0	0.0	3.979512998654303	0.0
23	3.000439564396184E-5	0.0	0.0	4.142676902166167	0.0
24	3.000439564396184E-5	0.0	0.0	4.276916568277253	0.0
25	3.000439564396184E-5	0.0	0.0	4.3944437860146515	0.0
26	3.000439564396184E-5	0.0	0.0	4.507740383966251	0.0
27	3.000439564396184E-5	0.0	0.0	4.628118019289826	0.0
28	3.000439564396184E-5	0.0	0.0	4.75515663044636	0.0
29	3.000439564396184E-5	0.0	0.0	4.889576322931309	0.0
30	3.000439564396184E-5	0.0	0.0	5.067562397891291	0.0
31	6.000879128792368E-5	0.0	0.0	5.226075620078341	0.0
32	6.000879128792368E-5	0.0	0.0	5.375407497198339	0.0
33	6.000879128792368E-5	0.0	0.0	5.527829827069666	0.0
34	6.000879128792368E-5	0.0	0.0	5.687483216291187	0.0
35	6.000879128792368E-5	0.0	0.0	5.867089528615942	0.0
36	6.000879128792368E-5	0.0	0.0	6.036794390378191	0.0
37	6.000879128792368E-5	0.0	0.0	6.2085095466485845	0.0
38	6.000879128792368E-5	0.0	0.0	6.387155718312733	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGT	20	7.858638E-4	44.0	37
CGGATTA	35	1.447188E-7	44.0	12
CGTCGGA	20	7.858638E-4	44.0	16
CGTTTTT	11225	0.0	41.863697	1
TACGACG	95	0.0	39.36842	1
TTACGCG	90	0.0	39.11111	1
TACGGGA	1625	0.0	38.72	4
GCGATAT	625	0.0	38.719997	9
ACGGGAC	1135	0.0	38.572685	5
TCGTAAG	195	0.0	38.358974	1
TAGGGAC	3880	0.0	38.103092	5
TACGCGG	245	0.0	37.714287	2
CGGTCTA	140	0.0	37.714287	31
TCGAACG	35	7.294373E-6	37.714287	1
GCGATGT	690	0.0	37.62319	9
AACGGGA	1435	0.0	37.560974	4
GTTACGG	325	0.0	37.23077	2
CGACGGT	130	0.0	37.230766	28
ACGATTG	130	0.0	37.230766	1
ACTACGG	160	0.0	37.125	2
>>END_MODULE