FastQCFastQC Report
Thu 26 May 2016
SRR1546640_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546640_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1685677
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60890.3612198541001627No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACG254.4437264E-544.01
TACCGAA254.4437264E-544.029
CGTTTTT61100.041.7315861
TGCGTAG900.041.5555571
GCGCGAC557.8216544E-1140.09
TAGAACG501.3478711E-939.6000021
CGGACGG1000.039.6000022
TGGTACG452.3521352E-839.111111
TTAGCGG4350.038.4367832
TACGGGA9000.038.3777774
TCGTAAG1150.038.260871
AGGGCGA17200.038.2441866
GCGATAC750.038.133339
CGTTTGG1850.038.0540542
GGCGATA6700.037.7611968
CGCGCGG1400.037.7142832
CCGCGCG357.292232E-637.7142831
AAGGGAT44750.037.6089365
GGGCGAT34050.037.6035277
GGCCGAT3750.037.5466658
Produced by FastQC (version 0.11.5)