##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546640_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1685677
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.109601661528277	31.0	31.0	33.0	30.0	34.0
2	31.48565353860793	31.0	31.0	34.0	30.0	34.0
3	31.631736091789826	31.0	31.0	34.0	30.0	34.0
4	35.39752870805024	37.0	35.0	37.0	33.0	37.0
5	35.21244164807374	37.0	35.0	37.0	32.0	37.0
6	35.29464126282793	37.0	35.0	37.0	32.0	37.0
7	35.80704073200263	37.0	35.0	37.0	35.0	37.0
8	35.85766430935464	37.0	35.0	37.0	35.0	37.0
9	37.646881935269924	39.0	37.0	39.0	35.0	39.0
10	36.918574554911764	39.0	37.0	39.0	32.0	39.0
11	36.50324528364568	39.0	35.0	39.0	32.0	39.0
12	36.161724933068434	38.0	35.0	39.0	32.0	39.0
13	36.053956362933114	38.0	35.0	39.0	31.0	39.0
14	37.04390936104603	39.0	35.0	41.0	31.0	41.0
15	37.197085206715165	39.0	35.0	41.0	32.0	41.0
16	37.21223164342872	39.0	35.0	41.0	32.0	41.0
17	37.12456360263562	39.0	35.0	41.0	32.0	41.0
18	37.05251955149178	38.0	35.0	40.0	32.0	41.0
19	37.04220203514671	39.0	35.0	40.0	32.0	41.0
20	36.92492452587299	38.0	35.0	40.0	31.0	41.0
21	36.85527476497573	38.0	35.0	40.0	31.0	41.0
22	36.78062819864066	38.0	35.0	40.0	31.0	41.0
23	36.58812453394096	38.0	35.0	40.0	31.0	41.0
24	36.559152791430385	38.0	35.0	40.0	31.0	41.0
25	36.428704906100045	38.0	35.0	40.0	30.0	41.0
26	36.355525999346256	38.0	35.0	40.0	30.0	41.0
27	36.201205213098355	38.0	35.0	40.0	30.0	41.0
28	36.1537845031996	38.0	35.0	40.0	30.0	41.0
29	36.076870005345036	38.0	35.0	40.0	30.0	41.0
30	36.075468787911326	38.0	35.0	40.0	30.0	41.0
31	36.0191590678404	38.0	35.0	40.0	30.0	41.0
32	35.83224188263825	38.0	34.0	40.0	29.0	41.0
33	35.64960309715325	38.0	34.0	40.0	28.0	41.0
34	35.569937775742325	38.0	34.0	40.0	27.0	41.0
35	35.37772123603751	38.0	34.0	40.0	27.0	41.0
36	35.20977150426802	38.0	34.0	40.0	26.0	41.0
37	35.299774511961665	38.0	34.0	40.0	26.0	41.0
38	35.27815055909287	38.0	34.0	40.0	26.0	41.0
39	35.21842025488869	38.0	34.0	40.0	26.0	41.0
40	35.21603545637747	38.0	34.0	40.0	26.0	41.0
41	35.25561658609568	38.0	34.0	40.0	26.0	41.0
42	35.0370219205696	38.0	34.0	40.0	25.0	41.0
43	35.09993432905592	38.0	34.0	40.0	26.0	41.0
44	34.93793828829604	38.0	34.0	40.0	25.0	41.0
45	34.778555440929665	38.0	34.0	40.0	24.0	41.0
46	34.84382891858879	38.0	34.0	40.0	24.0	41.0
47	34.73040861327526	38.0	33.0	40.0	24.0	41.0
48	34.77622225372951	37.0	34.0	40.0	24.0	41.0
49	34.68595229097864	37.0	33.0	40.0	24.0	41.0
50	34.21737794369859	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	11.0
15	36.0
16	81.0
17	248.0
18	626.0
19	1255.0
20	2322.0
21	3638.0
22	5854.0
23	8566.0
24	12641.0
25	17760.0
26	23893.0
27	29036.0
28	32952.0
29	38217.0
30	45967.0
31	56973.0
32	71172.0
33	90556.0
34	130058.0
35	169941.0
36	140648.0
37	176018.0
38	252395.0
39	374811.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.44411592493698	24.522906820227124	29.50950864252167	13.523468612314222
2	30.12184422045267	26.155129363454567	30.539124636570353	13.183901779522412
3	27.332163872438198	26.052144034711276	33.274227506218566	13.34146458663196
4	23.68828666464572	29.21864627683714	32.90594817393843	14.187118884578718
5	21.722133006501245	34.00176902217922	30.957413549570884	13.31868442174865
6	20.96119244671429	42.55483108567063	26.7214893481966	9.76248711941849
7	91.1197696830413	2.7135091716859163	5.233624235247915	0.9330969100248743
8	91.76164828730535	2.2121082508689387	4.923422458750994	1.102821003074729
9	86.16745675476382	5.866900954334668	6.226934341513825	1.7387079493876942
10	37.50510922317858	42.77361558590406	11.182154113747771	8.539121077169588
11	25.549022736858838	26.210418721973426	33.17895421246182	15.061604328705917
12	27.287078129439983	24.717131455195744	31.900595428424307	16.095194986939966
13	21.336827873904667	31.692489130479917	31.62711480313251	15.3435681924829
14	18.83753530480632	33.81145972804992	30.630957176256185	16.72004779088758
15	16.88704301001912	27.173651891791845	39.507687415797925	16.43161768239111
16	19.227349011702717	25.789638228438783	38.165022124641915	16.817990635216592
17	19.916626969460935	25.183946865265412	31.195596783962763	23.703829381310893
18	20.486605678312035	25.982439103102195	35.140243356230165	18.390711862355598
19	24.261290864145383	25.855605789246695	31.06852617672306	18.814577169884856
20	25.408307760027572	26.749430644186283	30.04537642739386	17.796885168392286
21	22.174651490172792	28.677261420782273	31.70328597946107	17.444801109583867
22	22.596737097320542	25.916827482370586	30.054571546031656	21.43186387427722
23	19.096185093585543	29.898135882497062	30.19967645047064	20.80600257344675
24	18.10560386123795	26.881069149071855	37.05348058969779	17.959846399992408
25	17.985414762140078	28.590827305587013	34.25822384715458	19.165534085118324
26	18.65001420794138	31.951554182681498	30.27377130968744	19.12466029968968
27	18.048890742413878	32.6804601356013	30.83906347420057	18.431585647784242
28	17.364773915762036	29.9801800700846	35.066801053819916	17.588244960333444
29	17.939913755719513	26.808635343544463	34.7996680265555	20.45178287418052
30	18.8543831350846	30.214744580367416	32.69274006823371	18.238132216314277
31	22.96555033971514	28.98289529963332	29.9377045543126	18.113849806338937
32	23.209131998597595	28.03941680404965	31.07878911558976	17.672662081762994
33	23.539503712751614	29.145915854579496	29.36428509139058	17.95029534127831
34	20.140394630762597	28.78392479698068	30.748358078089694	20.32732249416703
35	19.817556981556965	28.493121754642196	31.387863748511723	20.30145751528911
36	23.360347207679762	28.762153128980227	29.527364969682807	18.350134693657207
37	20.068376088657555	31.290098874220863	30.60372776041911	18.037797276702477
38	19.364979174539368	31.583927407207906	29.49544900950775	19.555644408744975
39	20.08587647574239	31.579062892831782	29.630943531886594	18.704117099539236
40	22.186456836036797	28.425552463490927	29.653783020115952	19.73420768035632
41	18.665556924606552	28.67637157059152	30.002544971545557	22.65552653325637
42	19.94670390590843	28.529724259155227	29.200493332945754	22.323078501990594
43	19.835769248794403	28.512105225378292	30.503886569016487	21.148238956810825
44	19.242239171561337	30.08915705677897	30.841495731388633	19.82710804027106
45	18.500815992624922	33.090087840078496	28.760314105252665	19.648782062043914
46	20.10515656320873	32.02108114425243	29.451905673506847	18.421856619032
47	19.81826886170957	29.620443299635696	29.809981390266344	20.751306448388394
48	20.50796208289014	28.341847222213985	31.764922936007313	19.385267758888567
49	20.56965836278243	27.91721071118607	31.900417458386155	19.612713467645342
50	19.579017807088782	32.01378437268824	29.6213924731725	18.78580534705047
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1247.0
1	1585.0
2	1923.0
3	7261.5
4	12600.0
5	9878.0
6	7156.0
7	7518.0
8	7880.0
9	8220.5
10	8561.0
11	8563.0
12	8565.0
13	8519.5
14	8474.0
15	8537.0
16	8600.0
17	8464.5
18	8329.0
19	8842.0
20	9355.0
21	10825.0
22	12295.0
23	13291.5
24	14288.0
25	18388.5
26	22489.0
27	27007.5
28	31526.0
29	38209.0
30	44892.0
31	51594.0
32	58296.0
33	65466.0
34	72636.0
35	80025.0
36	87414.0
37	93653.5
38	99893.0
39	109383.5
40	118874.0
41	129713.5
42	140553.0
43	147321.5
44	154090.0
45	153147.0
46	152204.0
47	143418.5
48	134633.0
49	123064.5
50	111496.0
51	99440.0
52	87384.0
53	78334.5
54	69285.0
55	61724.5
56	54164.0
57	48285.5
58	42407.0
59	37862.5
60	33318.0
61	28929.5
62	24541.0
63	19986.0
64	15431.0
65	12276.0
66	9121.0
67	6821.0
68	4521.0
69	3714.0
70	2907.0
71	2311.0
72	1715.0
73	1300.0
74	885.0
75	818.5
76	752.0
77	657.5
78	563.0
79	418.5
80	274.0
81	181.5
82	89.0
83	53.5
84	18.0
85	15.0
86	12.0
87	12.5
88	13.0
89	8.0
90	3.0
91	3.5
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1685677.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.504749136997166
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.56235145311322	16.464541613378103
2	5.914395574980019	2.5437518627382287
3	2.4275233640999536	1.5660984290750684
4	1.5493850854121956	1.3327655031357677
5	1.1121442817710672	1.1958191891816345
6	0.9008929449694952	1.1624086068515753
7	0.7714610233634288	1.1613053043481156
8	0.6685962303221838	1.1502395365616436
9	0.559554913237903	1.0829779233799788
>10	7.584619808833222	38.45810353198948
>50	1.588719843709431	23.524979719545417
>100	0.359244125455999	9.792745865458102
>500	5.55675365971236E-4	0.09989915213941959
>1k	2.77837682985618E-4	0.10055638340349471
>5k	2.77837682985618E-4	0.36380737881395087
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6089	0.3612198541001627	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02734806252917967	0.0
2	0.0	0.0	0.0	0.10120562836177986	0.0
3	0.0	0.0	0.0	0.14907956862435687	0.0
4	0.0	0.0	0.0	0.21872517688738707	0.0
5	0.0	0.0	0.0	0.32254103247537935	0.0
6	0.0	0.0	0.0	0.5008076873564746	0.0
7	0.0	0.0	0.0	0.5870045091675333	0.0
8	0.0	0.0	0.0	0.8336709820446029	0.0
9	0.0	0.0	0.0	0.9457327827335842	0.0
10	0.0	0.0	0.0	1.1058464937232935	0.0
11	0.0	0.0	0.0	1.2509513981622813	0.0
12	0.0	0.0	0.0	1.3698353836470452	0.0
13	0.0	0.0	0.0	1.4300485798880807	0.0
14	0.0	0.0	0.0	1.4629137135999364	0.0
15	0.0	0.0	0.0	1.496372080772295	0.0
16	0.0	0.0	0.0	1.5645346053840683	0.0
17	0.0	0.0	0.0	1.6426041287862385	0.0
18	0.0	0.0	0.0	1.745411487491376	0.0
19	0.0	0.0	0.0	1.7979126487458748	0.0
20	0.0	0.0	0.0	1.8583038150250613	0.0
21	0.0	0.0	0.0	1.9381530388087398	0.0
22	0.0	0.0	0.0	2.0260109143092064	0.0
23	0.0	0.0	0.0	2.1220554115646117	0.0
24	0.0	0.0	0.0	2.2015486952719887	0.0
25	0.0	0.0	0.0	2.268228136232505	0.0
26	0.0	0.0	0.0	2.3381110378797363	0.0
27	0.0	0.0	0.0	2.4153500344372025	0.0
28	0.0	0.0	0.0	2.4946653481064285	0.0
29	0.0	0.0	0.0	2.5750484820045596	0.0
30	0.0	0.0	0.0	2.6872882527316917	0.0
31	0.0	0.0	0.0	2.7855277137909575	0.0
32	0.0	0.0	0.0	2.8813349176621617	0.0
33	0.0	0.0	0.0	2.9796930254135283	0.0
34	0.0	0.0	0.0	3.081788503966062	0.0
35	0.0	0.0	0.0	3.201918279717882	0.0
36	0.0	0.0	0.0	3.3131495535621593	0.0
37	0.0	0.0	0.0	3.4338132394284315	0.0
38	0.0	0.0	0.0	3.548722560727826	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGACG	25	4.4437264E-5	44.0	1
TACCGAA	25	4.4437264E-5	44.0	29
CGTTTTT	6110	0.0	41.731586	1
TGCGTAG	90	0.0	41.555557	1
GCGCGAC	55	7.8216544E-11	40.0	9
TAGAACG	50	1.3478711E-9	39.600002	1
CGGACGG	100	0.0	39.600002	2
TGGTACG	45	2.3521352E-8	39.11111	1
TTAGCGG	435	0.0	38.436783	2
TACGGGA	900	0.0	38.377777	4
TCGTAAG	115	0.0	38.26087	1
AGGGCGA	1720	0.0	38.244186	6
GCGATAC	75	0.0	38.13333	9
CGTTTGG	185	0.0	38.054054	2
GGCGATA	670	0.0	37.761196	8
CGCGCGG	140	0.0	37.714283	2
CCGCGCG	35	7.292232E-6	37.714283	1
AAGGGAT	4475	0.0	37.608936	5
GGGCGAT	3405	0.0	37.603527	7
GGCCGAT	375	0.0	37.546665	8
>>END_MODULE